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EC number: 202-124-2 | CAS number: 92-09-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 Oct - 30 Nov 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-(2-(4-amino-N-ethyl-m-toluidino)ethyl)methanesulphonamide sesquisulphate
- EC Number:
- 247-161-5
- EC Name:
- N-(2-(4-amino-N-ethyl-m-toluidino)ethyl)methanesulphonamide sesquisulphate
- Cas Number:
- 25646-71-3
- Molecular formula:
- C12H21N3O2S.3/2H2O4S
- IUPAC Name:
- Methanesulfonamide, N-[2-[(4-amino-3-methylphenyl)ethylamino]ethyl]-, sulfate (2:3)
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: supernatant of activated sludge, non-adapted, domestic
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage. The supernatant liquid was used as inoculum.
- Storage conditions: Mineral components, Milli- RO water and inoculum was aerated with synthetic air overnight to purge the system of CO2.
- Storage length: Over night
- Pretreatment: The freshly obtained sludge was kept under continuous aeration until further treatment. Before use, the sludge was allowed to settle (41 min) and the supernatant liquid was then used as inoculum at the amount of 10 mL/L of mineral medium.
- Concentration of sludge: 4.3 g/L in the concentrated sludge. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 36.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline; tap-water purified by reverse osmosis (Milli- RO) and subsequently passed over activated carbon
- Test temperature: 22 - 23 °C
- pH: 7.5 - 7.9
- pH adjusted: yes
- Aeration of dilution water: aerated and stirred continuously at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
- Suspended solids concentration: 4.3 g/L in the concentrated sludge
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2 litre brown coloured glass bottles
- Number of culture flasks/concentration: 2 bottles (with test item)
- Method used to create aerobic conditions: A mixture of oxygen (ca. 20%) and nitrogen (ca. 80%) was passed through a bottle, containing 0.5 - 1 litre 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. The synthetic air was passed through the scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
- Measuring equipment: The CO2 produced in each test bottle reacted with the barium hydroxide in the gas scrubbing bottle and precipitated out as barium carbonate. The amount of CO2 produced was determined by titrating the remaining Ba(OH)2 with 0.05 M standardized HCl (1:20 dilution from 1 M HCl (Titrisol® ampoule), Merck, Darmstadt, Germany).
- Test performed in closed vessels: no
- Details of trap for CO2: emitted CO2 was trapped in 0.25 M NaOH. Two scrubbers containing 100 mL each were connected in series to the test vessels. The initial IC value of the 0.25 M NaOH was separately determined in each flask
SAMPLING
- Sampling frequency: Titrations were made every second or third day during the first 10 days, and thereafter at least every fifth day until day 28, for the inoculum blank and test item. Positive and toxicity control: at laest 14 days
- Sampling method: The CO2 produced in each test bottle reacted with the barium hydroxide in the gas scrubbing bottle and precipitated out as barium carbonate. The amount of CO2 produced was determined by titrating the remaining Ba(OH)2 with 0.05 M standardized HCl (1:20 dilution from 1 M HCl (Titrisol® ampoule), Merck, Darmstadt, Germany).
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 bottles
- Toxicity control: 1 bottle
- Other: positive control: 1 bottle
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 11
- Sampling time:
- 28 d
- Remarks on result:
- other: Vessel A
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 20
- Sampling time:
- 28 d
- Remarks on result:
- other: Vessel B
BOD5 / COD results
- Results with reference substance:
- The positive control item was biodegraded by at least 60% (73%) within 14 days.
Any other information on results incl. tables
Biological Results:
Table 1: CO2 Production and Percentage Biodegradation of the Positive Control Item
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 |
Produced CO2 |
Cumulative CO2 |
Biodegradation 1) |
|
|
Blank (mean) |
Positive Control |
(mL HCl) |
(mg) |
(mg) |
(%) |
2 |
47.08 |
45.21 |
1.87 |
2.1 |
2.1 |
2 |
5 |
46.19 |
22.01 |
24.18 |
26.6 |
28.6 |
33 |
8 |
45.69 |
32.09 |
13.60 |
15.0 |
43.6 |
51 |
12 |
45.65 |
35.41 |
10.24 |
11.3 |
54.9 |
64 |
152) |
46.23 |
39.14 |
7.09 |
7.8 |
62.7 |
73 |
1): Calculated as the ratio between CO2 produced (cumulative) and the ThCO2 of sodium acetate: 85.8 mg CO2/2L.
2): CO2 measured on day 15 is actually part of CO2 production of day 14, since microbial activity was ended on day 14 by addition of HCl.
Table 2: CO2 Production and Percentage Biodegradation of the Toxicity Control
Day |
HCl (0.05 N) titrated (mL)
|
Produced CO2 |
Produced CO2 |
Cumulative CO2 |
Biodegradation 1) |
|
|
Blank (mean) |
Toxicity control |
(mL HCl) |
(mg) |
(mg) |
(%) |
2 |
47.08 |
47.10 |
0.00 |
0.0 |
0.0 |
0 |
5 |
46.19 |
24.46 |
21.73 |
23.9 |
23.9 |
14 |
8 |
45.69 |
26.14 |
19.55 |
21.5 |
45.4 |
26 |
12 |
45.65 |
33.67 |
11.98 |
13.2 |
58.6 |
34 |
152) |
46.23 |
35.95 |
10.28 |
11.3 |
69.9 |
40 |
1): Calculated as the ratio between CO2 produced (cumulative) and the sum of the ThCO2 of the test item and positive control: 173.8 mg CO2/2L (ThCO2 test item: 88.0 mg CO2/2L + ThCO2 sodium acetate: 85.8 mg CO2/2L).
2): CO2 measured on day 15 is actually part of CO2 production of day 14, since microbial activity was ended on day 14 by addition of HCl.
Table 3: Comparison of Biodegradation of the Test Item in Bottles A and B
Day |
Biodegradation (%) |
|||
Bottle A |
Bottle B |
Mean A and B |
Δ A-B 1) |
|
2 |
0 |
1 |
1 |
1 |
5 |
1 |
2 |
2 |
1 |
8 |
3 |
4 |
4 |
1 |
12 |
6 |
6 |
6 |
0 |
15 |
7 |
10 |
9 |
3 |
19 |
8 |
13 |
11 |
5 |
23 |
9 |
16 |
13 |
7 |
29 2) |
11 |
19 |
15 |
8 |
29 2) |
11 |
20 |
16 |
9 |
29 2) |
11 |
20 |
16 |
9 |
1): Absolute difference in biodegradation between bottles A and B
2): Biodegradation is ended on day 28 by addition of HCl. Therefore, differences observed on day 29 are actually differences of day 28.
Table 3: Validity criteria for OECD 301 B.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
9% |
Yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
73% |
Yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
40% |
Yes |
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC. |
< 5% |
Yes |
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
38.6 CO2 /2 L 19.3 mg CO2/L |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In conclusion, the test item was not readily biodegradable under the conditions of the modified Sturm test presently performed.
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