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EC number: 219-547-3 | CAS number: 2459-10-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006 - 2007
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- other:
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- Trimethyl benzene-1,2,4-tricarboxylate
- EC Number:
- 219-547-3
- EC Name:
- Trimethyl benzene-1,2,4-tricarboxylate
- Cas Number:
- 2459-10-1
- Molecular formula:
- C12H12O6
- IUPAC Name:
- 1,2,4-trimethyl benzene-1,2,4-tricarboxylate
- Test material form:
- solid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9
- Test concentrations with justification for top dose:
- 5 - 2522 micrograms/mL
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
Any other information on results incl. tables
The highest concentrations chosen for the chromosome aberration assay were 1000 micrograms/mL for the 4 -hour exposure assay without a metabolic activation system, 2522 micrograms/mL
for the 4 hour exposure period with a metabolic activation system and 750 micrograms/mL for the 20 hour exposure period without a metabolic activation system.
A visible precipitate was observed at the beginning and end of the treatment periods at concentrations of 1000 micrograms/mL and greater.
Substantial toxicity (greater than a 50% reduction in cell growth relative to the vehicle control) was observed at the highest concentration in each test condition. The selection of doses for analysis was based on these dose concentration levels as well as precipitation. Cytogenetic evaluations were conducted at 500, 750 and 1000 microgramsg/mL for the 4 hour exposure period both with and without metabolic activation and at 100, 250 and 500 micrograms/mL for the 20 hour exposure period without metabolic activation.
The percentage of cells with structural aberrations following 4 hours exposure with metabolic activation was significantly increased above that of the vehicle control at 500, 750 and 1000 micrograms/mL. The percentage of cells with structural aberrations following 20 hours exposure without metabolic activation was also significantly increased above that of the vehicle control at 500 micrograms/mL. The observed changes were outside of the historical control range for structural aberrations, were accompanied by a concentration-related increase, and were therefore considered biologically significant. The percentage of cells with numerical aberrations following 4 hours exposure without metabolic activation showed a significant trend as compared to the vehicle control at 750 and 1000 micrograms/mL. The percentage of cells with numerical aberrations following 4 hours exposure with metabolic activation was also significantly increased above that of the vehicle control at 500, 750 and 1000 micrograms/mL. The observed changes were outside of the historical control range for numerical aberrations, and were therefore considered biologically significant.
Applicant's summary and conclusion
- Conclusions:
- A cytogenetic assay was positive for the induction of chromosome aberrations in Chinese hamster ovary cells.
- Executive summary:
A cytogenetic assay in Chinese hamster ovary cells resulted in positive findings for the induction of chromosome aberrations.
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