2-Chloro-1-fluoro-4-(trifluoromethyl)benzene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Study period:

2002-07-03 to 2002-07-05

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-1-fluoro-4-(trifluoromethyl)benzene

EC Number:

616-586-1

Cas Number:

78068-85-6

Molecular formula:

C7H3ClF4

IUPAC Name:

2-chloro-1-fluoro-4-(trifluoromethyl)benzene

impurity 1

Reference substance name:

1-Chloro-2-fluoro-4-(trifluoromethyl)benzene

Cas Number:

32137-20-5

Molecular formula:

C7H3ClF4

IUPAC Name:

1-Chloro-2-fluoro-4-(trifluoromethyl)benzene

Test material form:

liquid

Specific details on test material used for the study:

Purity: < 89.5 % [a/a]

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS (Clone 5)
Origin: Institut für Wasser-, Boden- und Lufthygiene des Bundesgesundheitsamtes, Corrensplatz, 14195 Berlin, FRG
Breeder: Dr.U.Noack-Laboratorieum, 31157 Sarstedt, FRG
Culture: In 2-3 L glass vessels with approx. 1.8 L culture medium, at 21°C in an incubator, 16 h illumination.
Culture medium: B.P.ELENDT M4, according to Selenium Deficiency in Crustacea, Protoplasma 154, 25-33, (1990).
Feeding: 5-7 times per week ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris.

Age of test organisms: 2-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

18-22°C

pH:

Test: 7.45-7.74
Control: 7.79

Dissolved oxygen:

Test: 7.24-7.88 mg/L
Control: 8.03 mg/L

Nominal and measured concentrations:

Nominal: 6.25, 12.5, 25, 50 and 100 mg/L
Measured: 1.23, 2.31, 4.31, 7.73 and 21.23 mg/L

Details on test conditions:

20 test organisms were exposed to each test concentration and control.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

10.9 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

10.1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

18.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks on result:

other: confidence interval

Remarks:

16.1 - 21.9

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

17.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks on result:

other: confidence interval

Remarks:

14.9 - 20.1

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 21.23 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 21.23 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50(48h) of 3-chloro-4-fluorobenzotrifluoride on Daphnia Magna (immobilisation) was determined at 17.3 mg/L.