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EC number: 410-610-2 | CAS number: 111850-24-9 MORTRACE SB CONC.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Although the study was conducted according to OECD TG 301B and EC Method C5 and in accordance with the Principles of Good laboratory Practices (GLP), additional biodegradation studies were not performed.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.5 (Degradation: Biochemical Oxygen Demand)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- The source of activated sludge obtained was from a municipal sewage treatment plant. The activated sludge was aerated for 4 hours and then left to settle for 1 hour. The supernatant was decanted to provide a sufficiently large volume for a 1% incolulum for each test flask.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Refer to attachments for more details
- Reference substance:
- other: sodium acetate
- Preliminary study:
- not applicable
- Test performance:
- The results were considered to be acceptable since the control substance degraded by at least 60% within 28 days and the total CO2 released in the blank at the end of the test was less than 50 mg CO2 per 3 litres medium.
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 21
- Sampling time:
- 28 d
- Remarks on result:
- other: low concentration - 10 mg/l
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 13
- Sampling time:
- 28 d
- Remarks on result:
- other: high concentration - 20 mg/l
- Details on results:
- The low concentration was 31.4 mg/l in 3 litre test medium and the high concentration was 63.4 mg/l in 3 litre test medium. Hence, the theoretical CO2 production following complete degradation would be 77.8 mg per 3 litre for the low and 157.2 mg per 3 litre for the high concentration.
The relative degradation values calculated from the measurements performed during the test period revealed significant biodegradation of Mortrace SB Conc. i.e. 21% at the low concentration (10 mg/l) and 13% at the high concentration (20 mg/l). As biodegradation was < 60% during the 28 days exposure period, Mortrace SB Conc. will not be considered 'readily biodegradable' under the conditions of the modified Strum test. - Results with reference substance:
- The positive control contained 59.2 mg sodium acetate resulting in a theoretcoal CO2 production following complete degradation of 63.5 mg per 3 litre.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- As biodegradation was < 60% during the 28 days exposure period, Mortrace SB Conc. will not be considered 'readily biodegradable' under the conditions of the modified Strum test.
- Executive summary:
Mortrace SB Conc. was tested for its ready biodegradability in the modified Strum test at nominal concentrations of 10 and 20 mg/l. The relative degradation values calculated from the measurements performed during the test period revealed significant biodegradation of Mortrace SB Conc. i.e. 21% at the low concentration (10 mg/l) and 13% at the high concentration (20 mg/l). As biodegradation was < 60% during the 28 days exposure period, Mortrace SB Conc. will not be considered 'readily biodegradable' under the conditions of the modified Strum test.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was conducted in compliance with Good Laboratory Practice Standards and following the requirements of the OECD Guidelines for the Testing of Chemicals No. 302C Inherent Biodegradability: Modified MITI Test II (Adopted: 17th July 1992) and The Guidelines for the Testing of Chemicals, SEPA, P.R. China No. 302C Inherent Biodegradability: Modified MITI Test II (May 2004). There were no deviations from the related regulations that affected the quality or integrity of the study. The original report was written in Chinese and was translated to English. The full report is not available at this time. Information entered is from a condensed report for this study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Fresh samples were collected from ten sites, mainly in areas where a variety of chemicals were used and discharged. From sites such as sewage treatment works, industrial waste-water treatment works, rivers, lakes, seas.
- Laboratory culture: Collect 1 litre samples of sludge, surface soil, water, etc. and mix thoroughly together. After removing floating matter and allowing to stand, adjusted the supernatant to pH 7±1 with sodium hydroxide or phosphoric acid. Used an appropriate volume of the filtered supernatant to fill a fill-and-draw activated sludge vessel and aerated the liquid for about 23.5 hrs. Thirty minutes after stopping aeration, discarded about one third of the whole volume of supernatant and added an equal volume of a solution (pH 7) containing 0.1 % each of glucose, peptone and potassium orthophosphate, to the settled material and re-commence aeration. This procedure was repeated once per day.
- Storage conditions: Temperature was kept at 25 ± 2°C and at pH 7 ± 1, sludge was sufficiently aerated to keep the mixture aerobic at all times.
- Storage length: One month.
- Concentration of sludge: 100 ppm (W/V) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 30 other: ppm
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
The following stock solutions were prepared using analytical grade reagents:
Solution (a): 2 I .75 g of dipotassium hydrogen phosphate, 8.5 g of potassium acid phosphate, 44.6 g of dibasic sodium phosphate dodecahydrate and 1.7 g of ammonium chloride were dissolved in water and the volume was made up to 1000 ml (The pH of the solution was 7.2).
Solution (b): 22.5 g of magnesium sulphate heptahydrate were dissolved in water and the volume was made up to 1000 ml.
Solution (c): 27.5 g of calcium chloride was dissolved in water and the volume was made up to 1000 ml.
Solution (d): 0.25 g of ferric chloride hexahydrate was dissolved in water and the volume was made up to 1000 ml.
Preparation of Mineral Medium:
Took 3 ml of each solution (a), (b), (c) and (d), and made up to 1000 ml. (distilled water was used throughout).
- Solubilising agent (type and concentration if used): Distilled water
- Test temperature: 20°C
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Normal laboratory equipment and especially the following were necessary: BOD-meter equipped with 6 bottles, pH-electrode and measuring equipment, bio-chemical incubator.
- Number of culture flasks/concentration: 6 bottles
- Method used to create aerobic conditions: Stirrers
SAMPLING
- Sampling frequency: Daily
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes - Reference substance:
- aniline
- Test performance:
- Performance of the Test
Test vessel 1 contained 155.8 ml of deionized water, to which 4.71 mg of test substance was added; test vessels 1, 2 contained 155.8 ml of mineral medium, to which 4.71 mg of test substance was added; test vessel 3 contained 155.8 ml of mineral medium, to which 15.7 mg of aniline was added; test vessel 4 was for the control blank test, containing only 155.8 ml of mineral medium. Test vessels 1 and 2 (test suspensions), 3 (activity control) and 4 (inoculum blank) were with a small volume of the inoculum to give a concentration of 100 mg/l suspended solids. The equipment was assembled and checked that it was air-tight. The stirrers were started and the measurement of oxygen uptake under conditions of darkness was initiated. The temperature and stirrer was checked on a daily basis. The oxygen uptakes were read for the six bottles directly.
According to the criterion of OECD Guidelines for the Testing of Chemicals and The Guidelines for the Testing of Chemjcals, SEPA, P.R. China, the test was considered valid. - Parameter:
- % degradation (O2 consumption)
- Value:
- 55.9
- Sampling time:
- 28 d
- Details on results:
- Treatment of Results:
Divided the oxygen uptake (mg) by the test substance (mg) after a given time, corrected for that taken up by the blank inoculum control after the same time, by the weight of the test substance used (mg). This yielded the BOD expressed as mg oxygen/mg test substance.
The percentage biodegradation was then obtained from:
% biodegradation = %ThOD = BOD (mg O2/mg substance)/ThOD (mg O2/mg substance) x 100
Results:
According to the calculation, the theoretic oxygen demanded of the test substance was 2.028 mg O2/mg test substance. The test results showed that, after 28 days test period finished, the percentage biodegradation of Marker 1 in activated sludge was 55.9%. - Parameter:
- BOD5
- Value:
- 0.7 mg O2/g test mat.
- Results with reference substance:
- The percentage degradation of aniline calculated from the oxygen consumption exceeded 40% after 7 days and 65% after 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Based on the results and the grading criteria with regard to ecology toxicity in the Guidelines for the Hazard Evaluation of New Chemical Substances, State Environmental Protection Agency of P.R.C. (HJ/T 154-2004) test substance Marker 1 had inherent and primary degradability, owing to its percentage biodegradation which was 55.9%, more than 20%.
- Executive summary:
The test assessed the inherent biodegradability of Marker 1 on micro-organisms in an aerobic aqueous medium by measuring its Biochemical Oxygen Demand (BOD). The test results showed that, after 28 days test period finished, the percentage biodegradation of Marker 1 in activated sludge was 55.9%.
Based on the result and the grading criteria with regard to ecology toxicity in the Guidelines for the Hazard Evaluation of New Chemical Substances, State Environmental Protection Agency of P.R.C. (HJ/T 154-2004) the test substance, Marker 1, was concluded that it had inherent and primary degradability, owing to its percentage biodegradation which was 55.9%, more than 20%.
Referenceopen allclose all
None
Description of key information
As biodegradation was < 60% during the 28 days exposure period, Mortrace SB Conc. is not be considered 'readily biodegradable' under the conditions of the modified Strum test. However, Mortrace SB Conc. is considered inherently biodegradable based on the results of the modified Miti Test.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, fulfilling specific criteria
Additional information
Mortrace SB Conc. was assessed for biodegradability in two, valid biodegradability tests. In one test, the ready biodegradability of the test material was assessed via the modified Strum test (OECD 301B) at nominal concentrations of 10 and 20 mg/l. The relative degradation values calculated from the measurements performed during the test period revealed significant biodegradation of Mortrace SB Conc. i.e. 21% at the low concentration (10 mg/L) and 13% at the high concentration (20 mg/L). As biodegradation was < 60% during the 28 days exposure period, Mortrace SB Conc. is not considered 'readily biodegradable' under the conditions of the modified Strum test. In a second biodegradation test, Mortrace SB Conc. was evaluated for inherent biodegradation in the Modified Miti Test (OECD 302C). Based on the observation of 55.9% biodegradability, the test substance is considered inherently biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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