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EC number: 812-958-4 | CAS number: 1078712-76-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 9, 2016 to February 22, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reference substance 001
- Cas Number:
- 1078712-76-1
- Molecular formula:
- C23-27H49-59N (Substance is a UVCB)
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were collected from each test chamber of each treatment and control group at the beginning and end of the first renewal and last renewal periods of the test to determine concentration of test substance. Newly prepared test solutions in each test chamber were sampled at 0 and 72 hours (± 1 hour), and
the 24-hour old solutions in the test chambers were sampled at 24 and 96 hours (±1 hour). Ten milliliter aliquots of samples were collected from mid-depth, placed in glass vials containing 10.0 mL of 0.2% formic acid in methanol, and processed immediately for analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was administered to the test organisms in water. This route of administration was selected because it represents the most likely route of exposure to aquatic organisms. Since the test substanceis poorly soluble in water, individual test solutions were prepared in the form of water accommodated fractions (WAF) for each loading rate of 6.3, 13, 25, 50 and 100 mg/L in 13.2 L Pyrex™ aspirators with tubulation and spigot approximately 2-3 cm from the bottom. Dilution water was pre-measured and emptied into labeled WAF bottles. For each loading rate, appropriate amounts of the test material were weighedand then rinsed with a portion of the dilution water into the appropriate WAF bottles. Each test solution was then stirred on a stir plate with a Teflon-lined stir bar for 23 hours, with a vortex depth of approximately 30% of the test solution height. The negative control was prepared in exactly same manner as the test solutions, but with dilution water only and no test substance. Due to the large amounts of oil droplets on the surface of the water in the 100 mg/L test solution, two batches of test solution were prepared. A small volume (approximately 5.5 L) was used from each batch in an effort to avoid allowing the large oil droplets into the test chamber when decanting.
At the beginning of the stirring process, the solutions in the WAF bottles appeared as follows. The negative control test solution appeared clear and colorless, while the 6.3, 13 and 25 mg/L solutions appeared clear and colorless with oil droplets on the water surface. The 50 and 100 mg/L WAF solutions appeared
translucent and colorless with increasing oil on the surface. The solutions were allowed to settle for approximately 1 hour to allow phase separation before decanting the aqueous phase. After the settling period and prior to decanting into the test chambers, the solutions in the WAF bottles appeared as follows. The
negative control appeared clear and colorless. The 6.3 and 13 mg/L WAF solutions appeared clear and colorless with a few oil droplets on surface. The 25, 50 and 100 mg/L WAF solutions appeared clear and colorless with many oil droplets on the surface, increasing in size (and stuck to the inside of the WAF bottle) with increasing concentration. Care was taken to avoid decanting the oil droplets on the water surface of the WAF solutions, that included the solutions being filtered through glass wool (placed in the spigot tubing) while decanting into their appropriate test chamber.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 144 mg/L as CaCO3
- Test temperature:
- 13 +/- 1 C
- pH:
- 8.0-8.2
- Dissolved oxygen:
- > 7.9 mg/L
- Conductivity:
- 346 usS/cm
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Results of analyses to measure concentrations of Amines, (2-ethylhexyl)(hydrogenated tallow
alkyl)methyl in the test solution samples collected during the test are presented in Table 1. Measured
concentrations from day 0, 24, 72 and 96 hour sample analysis ranged from approximately 0.022 to 18% of
nominal. When measured concentrations of the samples collected during the test were averaged, the mean
measured test concentrations for this study were 0.51, 0.17, 0.17, 0.65 and 0.23 mg/L, representing 8.1, 1.3,
0.68, 1.3 and 0.23% of nominal loading rates, respectively. Since the test substance is poorly soluble in
water, the individual test solutions prepared as water accommodated fractions (WAF) for each loading rate
were all above the solubility limit of the test substance. The resulting measured concentrations were variable,
but essentially equivocal despite differences in loading rate, and incorporating a lengthy mixing procedure.
Therefore, the results of the study were interpreted based on the nominal loading rates.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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