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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2019
Justification for type of information:
Please refer to read-across justification attached in IUCLID Section 13.
Reason / purpose for cross-reference:
read-across source
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Description of key information

In a 96-hour acute toxicity test on Zebrafish (Danio rerio) the source test item had no toxic effect on fish up to the limit concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L. The 96-h NOEC was determined to be 100 mg/L.

Key value for chemical safety assessment

Additional information

No data on the substance itself is available. Therefore, read-across was applied to the source substance Phosphoric acid, 2-ethylhexyl ester, sodium salt (CAS 68186-64-1). This approach is considered appropriate since both substances are UVCB substances consisting of a mono-, bis- and tris- substituted phosphate with C-chains (4 - 6 C), only the cation is different. In the target substance the cation is ammonium, while the source substance has sodium.

The acute toxicity of the source test item to fish (Zebrafish) was determined according to the OECD Guideline 203, EU Council Regulation No. 440/2008 method C.1 and EPA OPPTS 850.1075. A static test was performed as a limit test with a test concentration of 100 mg/L. The source item solution was prepared by mechanical dispersion – 0.5008 g test item were dissolved in 5008 mL ISO medium. Ten test organisms were exposed to the test concentration and the control, respectively, for 96 hours. The measured concentration of the source item at the start of the test was 91 mg/L and at the end of the test was 86 mg/L, respectively. The measured test item concentration remained within ± 20 % of the nominal during the test period of 96 hours. Therefore, the biological results are based on the nominal test item concentration. In conclusion, the source test item had no toxic effect on fish up to the limit concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L. The 96-h NOEC was determined to be 100 mg/L.

Based on the structural similarities between the source and the target substance, the target substance is also considered to show no toxicity to fish up to the limit concentration.