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EC number: 208-051-2 | CAS number: 506-68-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cyanogen bromide
- EC Number:
- 208-051-2
- EC Name:
- Cyanogen bromide
- Cas Number:
- 506-68-3
- Molecular formula:
- CBrN
- IUPAC Name:
- cyanogen bromide
- Test material form:
- solid: particulate/powder
Constituent 1
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Justification for species other than prescribed by test guideline: not relevant
- Source: Culture maintained at BRF, India.
- Age of parental stock (mean and range, SD): less than 24 h
- Feeding during test: NO
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- Range Finding: Control : 21.4 –21.5 ºC, Treatment : 21.5 – 21.9 ºC
Main Study: Control : 21.4 –21.5 ºC, Treatment : 21.4 –21.6 ºC - pH:
- Range Finding: Control: 7.0 – 7.1, Treatment: 6.0 – 6.8
Main Study: Control: 7.3 – 7.4, Treatment : 6.7 – 7.3 - Dissolved oxygen:
- Range Finding: Control : 3.7 – 3.8 mg/l, Treatment : 3.1 – 3.5 mg/l
Main Study: Control : 3.8 – 3.9 mg/l, Treatment : 3.5 – 3.8 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): not specifiedd
- Material, size, headspace, fill volume: glass
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10, 50 and 100mg/l
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.038 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 0.027 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based up on the result, Cyanogen Bromide exhibited EC50 value (95% confidence limit) of 0.0378 mg/l (Lower limit 0.0274 and Upper limit 0.0482 mg/l) in Daphnia magna for a period of 48 hour observation.
- Executive summary:
Acute immobilization effect of Cyanogen Bromide Sponsored by Libor Bachura vyzkum a vyroba was tested inDaphnia magnaat Bioscience Research Foundation, India. The study was conducted as per the OECD guideline No. 202.
Solubility and Stability Analysis
The test substance 100 mg wasdissolved in 1000 ml of exposure medium and found that it was soluble. Hence exposure medium was selected as vehicle and used as control.The test substance was stable in the exposure medium for 48 hours. Hence the study was conducted as static test.
Range finding study
A range finding study was conducted using control and five different test concentrations 0.1, 1, 10, 50 and 100mg/l. Each concentration consists of four replicates and each replicate contains 5 daphnids. On the day of exposure, physiochemical parameters of the treatment and control medium were within the range. The minimum and maximum range of pH (6.0 – 7.1), temperature (21.4º C – 21.9º C) and dissolved oxygen (3.1 – 3.8 mg/l) of the control and treatment medium was recorded. At the end of 24 hour observation 0, 30, 100, 100, 100 and 100% immobility was observed in the daphnids exposed to control, 0.1, 1, 10, 50 and 100 mg/l concentrations. Lethargy was observed in daphnids exposed to 0.1 mg/l concentration. No sublethal effect was observed in daphnids exposed to control. At the end of 48 hour observation 0 and 40% immobility was observed in the daphnids exposed to control and 0.1 mg/l concentration. Lethargy was observed in daphnids exposed to 0.1 mg/l concentration. No sublethal effect was observed in daphnids exposed to control.
Main study
Based on the range finding study results, the main study was conducted with 0.003125, 0.00625, 0.0125, 0.025 and 0.05 mg/l concentrations along with control againstDaphnia magna. Each concentration consists of four replicates and each replicate contains 5 daphnids. On day ‘0’ the physiochemical parameter of the treatment and control medium was within the range. The minimum and maximum range of pH (6.7 – 7.4), temperature (21.4 º C – 21.6 º C) and dissolved oxygen (3.5 – 3.9 mg/l) of the control and treatment medium was recorded. At the end of 24 hour observation 0, 0, 0, 5, 10 and 20% immobility was observed in the daphnids exposed to control, 0.003125, 0.00625, 0.0125, 0.025 and 0.05 mg/l concentration. Lethargy was observed in daphnids exposed to 0.0125, 0.025 and 0.05 mg/l concentration. No sublethal effect was observed in daphnids exposed to control, 0.003125 and 0.00625 mg/l concentration. At the end of 48 hour observation 0, 0, 10, 20, 30 and 35% immobility was observed in the daphnids exposed to control, 0.003125, 0.00625, 0.0125, 0.025 and 0.05 mg/l concentration. Lethargy was observed in daphnids exposed to 0.0125, 0.025 and 0.05 mg/l concentration. No sublethal effect was observed in daphnids exposed to control, 0.003125 and 0.00625 mg/l concentration.
Effective Concentration
Based on the immobility result, Cyanogen Bromide exhibited EC50value (95% confidence limit) of 0.0378 mg/l (Lower limit0.0274and Upper limit 0.0482 mg/l) for 48 hour exposure period.
LOEC
The Lowest Observed Effect Concentration (LOEC) was0.0274 mg/l estimated for 48 hour observation period.
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