9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-04-24 to 1989-04-26

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The strain of Daphnia magna used was obtained from Institut National de Recherche Chimique Appliquee, France, and bred since the beginning of 1978 in the Labor für Umweltanalytik und Ökologie of the BASF Ludwigshafen.
- Age of parental stock: 2 -24 h

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.89 mmol/L

Test temperature:

18.85 to 20.85

pH:

8.1

Conductivity:

600 - 700 µS/cm

Nominal and measured concentrations:

0, 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039 and 0.019 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel, test volume: Reagent tubes with fiat bottom, 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The synthetic medium M4 is used for the culture and test. The medium is prepared an the basis of an ultrapure, deionized water (conductivity < 0.05 µS/cm)

EFFECT PARAMETERS MEASURED: At the beginning and after 3, 6, 24, 48 h Daphnia magna's inability to swim is recorded. Those animals which are unable to swim after gentle agitation of the test vessels are regarded as unable to swim.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI: 0.12 - 0.2 mg/L

Table 1: Animals able to swim

Conc. [mg/L]	Animals able to swim after
	0 h	3 h	6 h	24 h	48 h
0.019	20	20	20	20	20
0.039	20	20	20	20	19
0.078	20	20	20	20	18
0.156	20	20	20	19	9
0.312	20	20	20	17	4
0.625	20	20	20	4	0
1.25	20	20	20	1	0
2.5	20	20	20	0	0
5	20	20	19	0	0
10	20	20	2	0	0
0	20	20	20	20	20

 

Conclusions:

Under the conditions of this acute daphnia test an EC50 of 0.16 mg/L (95% CI: 0.12 - 0.2 mg/L) was determined for the test item.

Executive summary:

The acute toxicity of the test substance to Daphnia magna was determined in a 48-hour static test conducted equivalent to OECD guideline 202. The daphnids were exposed to the following nominal test item concentrations: 0, 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039 and 0.019 mg/L. Under the conditions of this test, an EC50 of 0.16 mg/L (95% CI: 0.12 - 0.2 mg/L) was determined.

Description of key information

In an acute toxicity test with Daphnia magna conducted equivalent to OECD guideline 202 an 48h-EC50 of 0.16 mg/L was determined for the test item.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.16 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna was determined in a 48-hour static test conducted equivalent to OECD guideline 202. The daphnids were exposed to the following nominal test item concentrations: 0, 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039 and 0.019 mg/L. Under the conditions of this test, an EC50 of 0.16 mg/L (95% CI: 0.12 - 0.2 mg/L) was determined.