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EC number: 273-920-5 | CAS number: 69226-44-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 July 1992 to 11 December 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Study generally followed guideline, and met validity criteria. However, due to the insolubility of the test substance in the test medium, a constant factor between the test concentrations could not be achieved. It was not possible to determine an absolute EC50 (result is ≥ 100 mg/L) given that no inhibition was observed over the concentrations tested.
- Reason / purpose for cross-reference:
- other: read-across target
- Qualifier:
- according to guideline
- Guideline:
- other: Directive 87/302/EEC, part C, p. 118 (Biodegradation: Activated sludge respiration inhibition test)
- Deviations:
- yes
- Remarks:
- Instead of a centrifuged sludge a settled sludge was used. Due to the poor solubility of the test substance at test concentrations, no stock solution was prepared. The test substance was given directly into the medium. Therefore, a constant factor between
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- No samples were taken for analysis of the test material concentration. Only oxygen concentration was measured.
- Vehicle:
- no
- Details on test solutions:
- 20.1, 8.6, 3.9, 1.3, 0.6 and 0.3 mg of the test material were weighed and added to the test medium. The volume was adjusted to 200 mL with water and aerated for 3 hours. This produced test solutions of nominal concentrations of 100.5, 43.0, 19.5, 6.5, 3.0 and 1.5 mg/L. The sludge concentration in the test bottles was 1.60 g/L (dry weight) .
A reference compound (3,5-dichlorophenol) was also tested at 3 concentrations (32.0, 10.0, and 3.2 mg/L). - Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge collected from the sewage treatment plant of CH-4153 Reinach On 28/09/92. The pH after collection was 7.0. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. The pH of the sludge before use was 7.8.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none.
- Hardness:
- Not stated
- Test temperature:
- Temperature was maintained at 20 ± 2°C
- pH:
- pH of the sluge before use was 7.8. After 3 hours aeration the pH was 8.3 in all blank, reference and test concentration samples except blank sample 1 which was 7.8.
- Dissolved oxygen:
- Not stated
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Test material: 100.5, 43.0, 19.5, 6.5, 3.0 and 1.5 mg/L
20.1, 8.6, 3.9, 1.3, 0.6 and 0.3 mg of the test material were weighed and added to the test medium. The volume was adjusted to 200 mL with water and aerated for 3 hours.
Reference substance: 32, 10 and 3.2 mg/L. - Details on test conditions:
- DESIGN AND PROCEDURE
Vessels: 250 mL BOD flasks with gas inlet
Water: Dechlorinated drinking water
Temperature: 20 ± 2 °C
Nutrient solution: 16 g Peptone, 11 g Meat extract, 3.0 g Urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O, 2.8 g K2HPO4 are dissolved and made up to one litre with dechlorinated drinking water.
Deviations from guideline: Instead of a centrifuged sludge a settled sludge was used. Due to the poor solubility of the test material at test concentrations, no stock solution was prepared: The test material was given directly into the medium. Therefore, a constant factor between the test concentrations could not been achieved.
MEASUREMENTS
Oxygen comsumption per hour in mg/litre with a ORION - Electrode Type 97-08, ORION Microprocessor Ionalizer 901 and plotted on a recorder. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Compared to control oxygen consumption rates of 53.1 and 56.5 mg/L/hour, consumptions rates for the test concentrations of 100.5, 43.0, 19.5, 6.5, 3.0, and 1.5 mg/L were 50.5, 50.4, 52.6, 55.4, 54.8, and 58.0 mg oxygen/L/h respectively.
- Results with reference substance (positive control):
- The calculated EC50 for the reference substance, based on nominal concentrations, was 12.3 mg/L.
- Reported statistics and error estimates:
- The inhibitions were calculated on the basis of the measured time depend oxygen consumption of a Blank and Test solution. The Results ( ECxx Values ) were calculated with linear regression.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Study met the validity (quality) criteria of the guideline, though it deviated from the method in some minor ways (used settling instead of centrifugation for sludge, and test concentrations were not spaced by an equal factor). Because no significant inhibition of oxygen consumption was found over the test concentrations compared to the controls, resulting EC50 was ≥ 100 mg/L (the highest test concentration). Study provides no direct discussion of whether solubility prevented testing higher concentrations, though this is likely given that elsewhere in the report the poor solubility of the test compound is noted.
- Executive summary:
The determination of the inhibitory concentration of the test material on the respiration of aerobic waste water bacteria was performed in accordance with the 87/302/EEC directive.
The test was performed using activated sludge from a sewage treatment plant for a duration of 3 hours in an activated sludge respiration inhibition test.
Under the conditions of this study the LC50 of the test material was greater than 100 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted on read-across material
- Justification for type of information:
- Read-across to DOTE (Dioctyltin bis(2-ethylhexyl thioglycolate)) (CAS 15571-58-1), see attached justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Referenceopen allclose all
Table 1. Rates of inhibition (%) in control and test concentrations.
Sample | Conc (mg/l) | Consumption rate (mg/l/h) | Inhibition (%) |
Blank 1 | 0.0 | 53.1 | 3 |
Blank 2 | 0.0 | 56.5 | -3 |
Reference 1 | 32.0 | 11.1 | 80 |
Reference 2 | 10.0 | 31.4 | 43 |
Reference 3 | 3.2 | 50.0 | 9 |
Test conc. 1 | 100.5 | 50.5 | 8 |
Test conc. 2 | 43.0 | 50.4 | 8 |
Test conc. 3 | 19.5 | 52.6 | 4 |
Test conc. 4 | 6.5 | 55.4 | -1 |
Test conc. 5 | 3.0 | 54.8 | 0 |
Test conc. 6 | 1.5 | 58.0 | 6 |
Description of key information
Read-across to structurally similar substance DOTE (CAS No 15571 -58 -1)
Under the conditions of the study the LC50 of DOTE (CAS No 15571-58-1) was greater than 100 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
Read-across to structurally similar substance (DOTE) (CAS No 15571-58-1).
The determination of the inhibitory concentration of the test material on the respiration of aerobic waste water bacteria was performed in accordance with the 87/302/EEC directive.
The test was performed using activated sludge from a sewage treatment plant for a duration of 3 hours in an activated sludge respiration inhibition test.
Under the conditions of this study the LC50 of the test material was greater than 100 mg/L.
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