Hydroxyoctadecanoic acid, monoester with glycerol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 February to 09 February

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test substance, Glyceryl Monohydroxystearate (Synonyms: GMHS, Naturechem GMHS), an off-white solid, was received from EAG Laboratories (formerly Wildlife International), Easton, Maryland on 29 November 2017 and was stored at room temperature.

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

The biological response results were reported based upon the nominal GMHS loading rate concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna neonates (<24-h old) were obtained from an in house daphnid culture. All daphnids were cultured in a temperature-controlled area at approximately 20°C.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

144 mg CaCO3/L

Test temperature:

20.1°C to 20.3°C

pH:

8.5 to 8.6

Dissolved oxygen:

8.4 to 9.2 mg/L (97 to 106% saturation)

Conductivity:

339 µS/cm

Nominal and measured concentrations:

The substance was tested at five nominal concentration levels of 6.3 mg/l, 13 mg/l, 25 mg/l, 50mg/l, and 100 mg/l.

Details on test conditions:

16-hr light:8-hr dark

Reference substance (positive control):

no

Key result

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Remarks:

None of the control daphnids showed immobilization or other signs of disease or stress and the oxygen concentration at the end of the test was equal to or greater than 3 mg/L (8.4 to 8.7 mg/L) in the control and test vessels.

Conclusions:

The test substance did not induce mortality of Daphnia magna at nominal concentrations of 6.3 mg/L, 13 mg/L, 25 mg/L, 50 mg/L, and 100 mg/L after 48 h of exposure. The 48-h NOEC, 48-h LOEC and 48-hr EC50 were each reported as ≥ 100 mg/L.

Description of key information

The key study was a static acute invertebrate toxicity test on the effects of the substance on the freshwater invertebrate Daphnia magna following OECD Guideline 202. The test substance did not induce acute immobilisation of Daphnia magna at nominal concentrations of 6.3 mg/L, 13 mg/L, 25 mg/L, 50 mg/L and 100 mg/Lafter 48 h of exposure. The 48-h NOEC was > 100 mg/L, the 48-h LOEC was >100 mg/L and the 48-hr EC50 was > 100mg/L. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L