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EC number: 252-390-9 | CAS number: 35141-30-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-09-07 to 1994-10-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from a primarily communal sewage treatment plant (Marl- East)
- Preparation of inoculum for exposure: washed
- Concentration of sludge: 27.6 g/ml - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10.7 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 21.9-22.2°C
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2L Erlenmeyer flasks with loose closure of aluminium foil
- Number of culture flasks/concentration: two test substance flasks, two control flasks, two innoculum blank flasks.
SAMPLING
- Sampling frequency: 0 hours, 3 hours, 7, 14, 21, 27, 28 days
- Sampling method: samples were centrifuged at 3000RPM for 15 min, then duplicate determination of oxygen-enriched and de-gassed samples
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 10.8 mg/L DOC
- Parameter:
- % degradation (DOC removal)
- Value:
- 39
- Sampling time:
- 28 d
- Details on results:
- Biodegradation of reference substance was 98% after 7 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- A biodegradation result of 39% in 28 days (DOC) was determined in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the attached justification for grouping of substances in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Parameter:
- % degradation (DOC removal)
- Value:
- 39
- Sampling time:
- 28 d
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- One study is available for the source substance CAS 1760-24-3. A biodegradation result of 39% in 28 days (DOC) was determined in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP. As explained in the attached justification the target and the source substances are not readily biodegradable.
Referenceopen allclose all
Table 1: Dissolved organic carbon levels (mg/l) and calculated % DOC reduction
Type of suspension |
0 |
3 hours |
7 |
14 |
21 |
27 |
28 |
|||||||
DOC (mg/l) |
DOC reduction (%) |
DOC (mg/l) |
DOC reduction % |
DOC (mg/l) |
DOC reduction (%) |
DOC (mg/l) |
DOC reduction (%) |
DOC (mg/l) |
DOC reduction (%) |
DOC (mg/l) |
DOC reduction (%) |
DOC (mg/l) |
DOC reduction (%) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Test sample - median value flask 1 |
8.49 |
0 |
8.27 |
0 |
4.77 |
47.59 |
4.76 |
45.81 |
4.48 |
48.98 |
4.71 |
48.10 |
5.21 |
41.75 |
Test sample - median value flask 2 |
8.88 |
0 |
8.37 |
0 |
4.97 |
45.74 |
4.54 |
49.25 |
4.49 |
49.50 |
4.47 |
51.75 |
5.74 |
35.84 |
Test sample - Mean value |
|
0 |
|
0 |
|
47 |
|
48 |
|
49 |
|
50 |
|
39 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Blank mean value |
0.51 |
- |
0.39 |
|
0.64 |
- |
0.49 |
- |
0.46 |
- |
0.62 |
- |
0.62 |
- |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Reference substance – median value flask 1 |
11.64 |
0 |
11.89 |
0 |
0.82 |
98.43 |
0.64 |
98.70 |
0.51 |
99.57 |
0.72 |
99.13 |
0.74 |
98.96 |
Reference substance – median value flask 2 |
11.31 |
0 |
11.44 |
0 |
0.83 |
98.28 |
0.51 |
99.82 |
0.52 |
99.46 |
0.76 |
98.73 |
0.82 |
98.19 |
Median value |
|
0 |
|
0 |
|
98 |
|
100 |
|
100 |
|
99 |
|
99 |
Description of key information
Not readily biodegradable (39% within 28 d, EU Method C.4-A) based on read across from CAS 1760-24-3.
Key value for chemical safety assessment
Additional information
Experimental data on the biodegradability of N-(2-aminoethyl)-N’-[3-(trimethoxysilyl)propyl]ethylenediamine (CAS 35141-30-1) are not available. Therefore, in accordance with the Regulation (EC) No. 1907/2006 Annex XI, 1.5 “Grouping of substances and read across” and the read across assessment framework (RAAF, ECHA 2017) a read across from the analogue substance N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (CAS 1760-24-3) has been performed. Details on the read-across justification can be found in the attached justification in the respective target entry and in the overall justification for grouping of substances attached in IUCLID Section 13.
The biodegradation study (1994) with the source substance was performed according to EU Method C.4-A and GLP. Domestic, non-adapted activated sludge was exposed to an initial test item concentration of 10.7 mg/L DOC. Measured DOC removal revealed 39% biodegradation of the test item after 28 d. Based on the rapid hydrolysis of the test substance (half-life < 10 min), the observed biodegradation is attributed to the hydrolysis products. On this basis, the test item was not readily biodegradable under the testing conditions.
Based on these results and the attached read across justification (see attachment in respective target entry in IUCLID) the target substance N-(2-aminoethyl)-N'-[3-(trimethoxysilyl)propyl]ethylenediamine (CAS 35141-30-1) is not considered readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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