N-(2-aminoethyl)-N'-[3-(trimethoxysilyl)propyl]ethylenediamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-09-07 to 1994-10-05

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from a primarily communal sewage treatment plant (Marl- East)

- Preparation of inoculum for exposure: washed

- Concentration of sludge: 27.6 g/ml

Duration of test (contact time):

28 d

Initial conc.:

10.7 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS

- Test temperature: 21.9-22.2°C

- Continuous darkness: yes

TEST SYSTEM

- Culturing apparatus: 2L Erlenmeyer flasks with loose closure of aluminium foil

- Number of culture flasks/concentration: two test substance flasks, two control flasks, two innoculum blank flasks.

SAMPLING

- Sampling frequency: 0 hours, 3 hours, 7, 14, 21, 27, 28 days

- Sampling method: samples were centrifuged at 3000RPM for 15 min, then duplicate determination of oxygen-enriched and de-gassed samples

CONTROL AND BLANK SYSTEM

- Inoculum blank: yes

Reference substance:

benzoic acid, sodium salt

Remarks:

10.8 mg/L DOC

Parameter:

% degradation (DOC removal)

Value:

39

Sampling time:

28 d

Details on results:

Biodegradation of reference substance was 98% after 7 days.

Table 1: Dissolved organic carbon levels (mg/l) and calculated % DOC reduction

Type of suspension	0		3 hours		7		14		21		27		28
	DOC (mg/l)	DOC reduction (%)	DOC (mg/l)	DOC reduction %	DOC (mg/l)	DOC reduction (%)	DOC (mg/l)	DOC reduction (%)	DOC (mg/l)	DOC reduction (%)	DOC (mg/l)	DOC reduction (%)	DOC (mg/l)	DOC reduction (%)
Test sample - median value flask 1	8.49	0	8.27	0	4.77	47.59	4.76	45.81	4.48	48.98	4.71	48.10	5.21	41.75
Test sample - median value flask 2	8.88	0	8.37	0	4.97	45.74	4.54	49.25	4.49	49.50	4.47	51.75	5.74	35.84
Test sample - Mean value		0		0		47		48		49		50		39
Blank mean value	0.51	-	0.39		0.64	-	0.49	-	0.46	-	0.62	-	0.62	-
Reference substance – median value flask 1	11.64	0	11.89	0	0.82	98.43	0.64	98.70	0.51	99.57	0.72	99.13	0.74	98.96
Reference substance – median value flask 2	11.31	0	11.44	0	0.83	98.28	0.51	99.82	0.52	99.46	0.76	98.73	0.82	98.19
Median value		0		0		98		100		100		99		99

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

A biodegradation result of 39% in 28 days (DOC) was determined in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to the attached justification for grouping of substances in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Parameter:

% degradation (DOC removal)

Value:

39

Sampling time:

28 d

Interpretation of results:

not readily biodegradable

Conclusions:

One study is available for the source substance CAS 1760-24-3. A biodegradation result of 39% in 28 days (DOC) was determined in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP. As explained in the attached justification the target and the source substances are not readily biodegradable.

Description of key information

Not readily biodegradable (39% within 28 d, EU Method C.4-A) based on read across from CAS 1760-24-3.

Additional information

Experimental data on the biodegradability of N-(2-aminoethyl)-N’-[3-(trimethoxysilyl)propyl]ethylenediamine (CAS 35141-30-1) are not available. Therefore, in accordance with the Regulation (EC) No. 1907/2006 Annex XI, 1.5 “Grouping of substances and read across” and the read across assessment framework (RAAF, ECHA 2017) a read across from the analogue substance N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (CAS 1760-24-3) has been performed. Details on the read-across justification can be found in the attached justification in the respective target entry and in the overall justification for grouping of substances attached in IUCLID Section 13.

The biodegradation study (1994) with the source substance was performed according to EU Method C.4-A and GLP. Domestic, non-adapted activated sludge was exposed to an initial test item concentration of 10.7 mg/L DOC. Measured DOC removal revealed 39% biodegradation of the test item after 28 d. Based on the rapid hydrolysis of the test substance (half-life < 10 min), the observed biodegradation is attributed to the hydrolysis products. On this basis, the test item was not readily biodegradable under the testing conditions.

Based on these results and the attached read across justification (see attachment in respective target entry in IUCLID) the target substance N-(2-aminoethyl)-N'-[3-(trimethoxysilyl)propyl]ethylenediamine (CAS 35141-30-1) is not considered readily biodegradable.