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EC number: 227-774-4 | CAS number: 5977-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
6 albino rabbits were tested for skin irritation with Acetoacet Amide according to a company guideline similar to OECD 404. Following this guideline 0.5 g of the test item were tested at the intact and abraded skin under occlusive coverage.
The exposure period was 4h. After removal of the patch the remnant test item was removed with appropriate solvent. The observation period was 48 hours after initial application.
None of the animals showed any signs of skin irritation or corrosion at any time.
Acetessigamidlösung ca. 30 %ig was tested for eye irritating proprties according to OECD 405. Following this protocol the left eyes of three rabbits were treated with 0.1 ml of 30 % aequous acetoacetamid solution.
The untreated right eyes served as control. 24 hours after instillation the treated eyes were washed with physiological saline (37°C). The examination of the eyes was performed 1, 24, 48 and 72 hours after application.
No signs of irritation were observed at any time.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1974
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Study conducted prior to GLP and OECD guideline implementation.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- DOT (Transport-Classification) Guideline
- Deviations:
- not specified
- GLP compliance:
- no
- Remarks:
- Study conducted prior to GLP and OECD guideline implementation.
- Specific details on test material used for the study:
- not available
- Species:
- rabbit
- Strain:
- not specified
- Details on test animals or test system and environmental conditions:
- albino rabbits
- Type of coverage:
- occlusive
- Preparation of test site:
- abraded
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- 0.5 g
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 48 hours after initial application
- Number of animals:
- 6
- Details on study design:
- The intact and abraded skin of 6 albino rabbits was employed for this study. The hair was clipped from the backs with the aid of angora clippers. Four areas of the back, placed approximately ten centimeters apart, were designated for the patches. Areas 2 and 3 were abraded by making four epidermal incisions (two perpendicular to two others in the area of the patch). The patches consisted of 1.5 inch x 1.5 inch 12 ply gauze squares. The patches were secured to the area by thin bands of adhesive tape. The material to be tested (0.5 grams) was introduced beneath the patch. The entire trunks of the animlas were then wrapped in claear plastic trunk bands. The trunk bands help to hold the patches in position and retard evaporation of volatile substances during the four hours exposure period. Upon removal of the patches the resulting reactions were evaluated on basis of weighted scores.
Following this initial reading, all test sites were washed with appropriate solvent to prevent further exposure. Readings were again made at 24 and 48 hours after the iniitial application. Each test substance is evaluated on a total of site (6 abraded and 6 intact) - Irritation parameter:
- erythema score
- Basis:
- animal: 1-6
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks:
- no signs of irritation (erythema) at any time
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- edema score
- Basis:
- animal: 1-6
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks:
- no signs of irritation (erythema) at any time
- Remarks on result:
- no indication of irritation
- Other effects:
- No other effects reported.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Based on the findings, Acetoacet Amide is not irritating to the rabbit skin.
- Executive summary:
6 albino rabbits were tested for skin irritation with Acetoacet Amide according to a company guideline similar to OECD 404. Following this guideline 0.5 g of the test item were tested at the intact and abraded skin under occlusive coverage.
The exposure period was 4h. After removal of the patch the remnant test item was removed with appropriate solvent. The observation period was 48 hours after initial application.
None of the animals showed any signs of skin irritation or corrosion at any time.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 Mai 1989 - 2 June 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, breeding colony and Madörin AG, Füllingsdorf, Schweiz
- Age at study initiation: about 3 - 5 months
- Weight at study initiation: 2,6 - 3,3 kg
- Housing: in air conditioned rooms in single cages
- Diet: Altromin 2123 Haltungsdiät - Kaninchen, ad libitum. 15 grams hay per day
- Water : deionized and chlorinated water from automatic suspensors, ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +- 3
- Humidity (%): 50 +- 20
- Photoperiod 12 hrs dark / 12 hrs light - Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 0,1 ml of the 30% aequous acetoacetamide-solution
- Duration of treatment / exposure:
- 24 hours
- Observation period (in vivo):
- 72 hours
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- REMOVAL OF TEST SUBSTANCE:
Washing with physiological saline (37 °C) 24 hours after application of the test item and before each investigation
SCORING SYSTEM:
Cornea: 0 - 4
Iris: 0 - 2
Conjunctivae: 0 - 3
Chemosis: 0 - 4 - Irritation parameter:
- cornea opacity score
- Basis:
- other: animal # 100, 182 and 543
- Remarks:
- each
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- other: animal # 100, 182 and 543
- Remarks:
- each
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Basis:
- other: animal # 100, 182 and 543
- Remarks:
- each
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- other: animal # 100, 182 and 543
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Other effects:
- No other effects reported
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Based on the findings, Acetessigamidlösung (ca. 30 %ig) is not irritating to the rabbit eye.
- Executive summary:
Acetessigamidlösung ca. 30 %ig was tested for eye irritating proprties according to OECD 405. Following this protocol the left eyes of three rabbits were treated with 0.1 ml of 30 % aequous acetoacetamid solution.
The untreated right eyes served as control. 24 hours after instillation the treated eyes were washed with physiological saline (37°C). The examination of the eyes was performed 1, 24, 48 and 72 hours after application.
No signs of irritation were observed at any time.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for classification or non-classification
Based on the irritation/corrosion data available, the substance is not classified as irritating and does not require labelling according to Regulation 1272/2008/EEC (CLP).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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