Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 613-953-8 | CAS number: 66603-10-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Principles of method if other than guideline:
- The acute oral toxicity of K-HDO in Sprague-Dawley rats has been determined. For this purpose, the product was administered once by gavage as 0.562 - 2.61 % w/v aqueous solutions in doses of 56.2, 68.1, 82.5, 100, 121, 147, 178, 215 and 261 mg/kg.
- GLP compliance:
- no
- Remarks:
- GLP was not compulsory at the time the study was performed, however the conditions of good laboratory practice have always been observed.
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- EC Number:
- 613-953-8
- Cas Number:
- 66603-10-9
- Molecular formula:
- C6H11KN2O2
- IUPAC Name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- Test material form:
- solid: particulate/powder
- Details on test material:
- Test item: (N-Cyclohexyl-diazeniumdioxy)-potassium
IUPAC name: Cyclohexylhydroxydiazene 1-oxide, potassium salt
Chemical name: Cyclohexylhydroxydiazene 1-oxide, potassium salt; synonyma: (N-Cyclohexyl-diazeniumdioxy)-potassium, K-HDO, K-NCH, Xyligen K powder, Xyligen K
Molecular formula: C6 H11 K N2 O2
Molecular mass: 182.27
Constituent 1
- Specific details on test material used for the study:
- Chemical name: N-Cyclohexyldiazeniumdioxy potassium
Test substance number: XXV/210
Degree of purity: analytical grade
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: WIGA, Sulzfeld, FRG
- Sex: male / female
- Weight at study initiation: male: 203 g; female: 163 g
- Fasting period before study: Food was withdrawn about 16 h before administration.
- Diet / water (e.g. ad libitum): The animals received Altromin R 1324 supplied by ALTROMIN GmbH, Lage/Lippe, FRG and water ad libitum.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: aqueous solution
- Details on oral exposure:
- The product was administered once by gavage in various doses as 0.562 - 2.61 % w/v aqueous solutions.
- Doses:
- Test doses: 56.2, 68.1, 82.5, 100, 121, 147, 178, 215, 261 mg/kg
Test concentrations: 2.61, 2.15, 1.78, 1.47, 1.21, 1, 0.825, 0.681, 0.562 % w/v - No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 1, 24 and 48 hours and 7 and 14 days
- Examinations performed: signs of toxicity and necropsy of the animals which died and those which were sacrificed with carbon dioxide at the end of the observation period. - Statistics:
- Determination of the median Lethal dose (LD 50), calculated by FINNEY'S Probit analysis for a Log-normal distribution.
Results and discussion
- Preliminary study:
- no preliminary study performed
Effect levelsopen allclose all
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 150 mg/kg bw
- Based on:
- test mat.
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 122 mg/kg bw
- Based on:
- test mat.
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 136 mg/kg bw
- Based on:
- test mat.
- Mortality:
- see table below
- Clinical signs:
- other: see table below
- Gross pathology:
- Results of necropsy are given in the table below.
- Other findings:
- All findings are summarized in the tables below.
Any other information on results incl. tables
Mortality:
Dose (mg/kg) |
Cone. (%) |
Number of animals |
died within |
||||
1 h |
24 h |
48 h |
7 days |
14 days |
|||
261 |
2.61 |
10 m 10 f |
1/10 0/10 |
6/10 3/10 |
6/10 4/10 |
8/10 7/10 |
8/10 9/10 |
215 |
2.15 |
10 m 10 f |
3/10 0/10 |
7/10 7/10 |
8/10 7/10 |
8/10 8/10 |
8/10 9/10 |
178 |
1.78 |
10 m 10 f |
1/10 1/10 |
6/10 1/10 |
8/10 2/10 |
8/10 2/10 |
8/10 3/10 |
147 |
1.47 |
10 m 10 f |
2/10 2/10 |
4/10 3/10 |
4/10 3/10 |
5/10 4/10 |
5/10 5/10 |
121 |
1.21 |
10 m 10 f |
1/10 1/10 |
3/10 1/10 |
3/10 2/10 |
8/10 2/10 |
8/10 2/10 |
100 |
1 |
10 m 10 f |
0/10 1/10 |
1/10 1/10 |
1/10 2/10 |
2/10 2/10 |
2/10 3/10 |
82.5 |
0.825 |
10 m 10 f |
2/10 1/10 |
2/10 2/10 |
2/10 2/10 |
4/10 2/10 |
4/10 2/10 |
68.1 |
0.681 |
10 m 10 f |
0/10 1/10 |
0/10 1/10 |
1/10 1/10 |
1/10 1/10 |
1/10 1/10 |
56.2 |
0.562 |
10 m 10 f |
0/10 0/10 |
0/10 0/10 |
0/10 0/10 |
1/10 0/10 |
1/10 0/10 |
Applicant's summary and conclusion
- Conclusions:
- The determined LD50 value of male and female rats for acute oral toxicity amounts to 136 mg K-HDO/kg bw corresponding to 452 mg (30% w/w K-HDO)/kg bw
- Executive summary:
The median lethal dose (LD50), calculated by FINNEY's probit analysis for a log-normal distribution, determined in this study was:
for male and female animals after 14d: 136 (117 - 161) mg/kg
for male animals after 14d: 122 (92 - 161) mg/kg
for female animals after 14d: 150 (120 - 202) mg/kg
Clinical signs (summary):
Immediately after administration strong neurological effects were observed; At the low dose group clonic spasms, twitching, dyspnoea and poor general condition. With increasing doses also tremor, tonic spasms, saltatory spasms, salivation, staggering, spastic gate, lateral position, apathy and agitation were observed.
The determined LD50value of male and female rats for acute oral toxicity amounts to 136 mg K-HDO/kg bw corresponding to 452 mg (30% w/w K-HDO)/kg bw
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.