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EC number: 230-989-6 | CAS number: 7394-38-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3 October 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study according to test guideline OECD 209 (not performed according to GLP). The test report is with detailed documentation. Further details on the test item are lacking in the report.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Final test concentrations were prepared with test medium by dilution of stock solutions of the test substance.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: ARA Werdhölzli, CH-8048 Zürich (03 October 2001; 9.30 a.m.)
- Preparation of inoculum for exposure: The activated sludge was used immediately after sampling from the treatment plant without adaptation.
lf the sludge was used on the following day, 50 ml of the synthetic sewage concentrate were added per liter of activated sludge and the sludge aerated overnight. Prior to the test, the sludge was washed twice with tap water. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 22 +/- 0.5 °C
- pH:
- 7.5 +/- 0.5
- Nominal and measured concentrations:
- Nominal: 0, 125, 250, 500, 1000, 2000 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 ml flask
- Material, size, headspace, fill volume: total volume of test suspension of 500 ml
- Aeration: aerated by shaking (120 r.p.m.)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per reference compound concentration (replicates): 1
- Sludge concentration (weight of dry solids per volume): 1.6 g/l dry matter in the final mixture
- Nutrients provided for bacteria:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic sewage feed prepared with tap water: 16 ml of 100-fold sewage concentrate per 500 ml of final test mixture
OTHER TEST CONDITIONS
- Adjustment of pH: The pH-value was checked and adjusted to pH 7.5 +/- 0.5 with NaOH or H2SO4, if necessary
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): oxygen consumption
TEST CONCENTRATIONS
- Test concentrations: 0, 125, 250, 500, 1000 and 2000 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol, 3, 10 and 30 mg/l
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- At the applied test concentrations, the test substance showed no significant toxic effects (<15 %) to the activated sludge. The respiration rates were in the same range as determined for the two blank controls.
- Results with reference substance (positive control):
- The EC50 value of the reference substance 3,5-dichlorophenol was estimated to be 3 mg/l which was low but in the range of 5 mg/l thus confirming suitability of activated sludge and test conditions.
- Validity criteria fulfilled:
- yes
- Remarks:
- The EC50 value of the reference substance 3,5-dichlorophenol was estimated to be 3 mg/l which was low but in the range of 5 mg/l. The variation between the two controls was + 3.1 %. Therefore, the test was considered valid.
- Conclusions:
- Based on these data the EC20, EC50, EC80 values of the test material were estimated to be >2000 mg/l.
- Executive summary:
The inhibitory effect of the test material on the oxygen consumption of activated sludge microorganisms of a municipal sewage treatment plant was investigated under aerobic static conditions. The inhibitory effect may include the effect on respiration and nitrification. The exposure time of the test substance on the activated sludge was 3 hours.
The nominal concentrations of the test material were 125, 250, 500, 1000 and 2000 mg/l, respectively.
These test concentrations were prepared by corresponding dilution of stock solutions of the test substance.
No chemical analysis of test concentrations was conducted. For the calculation of the effective concentration leading to 20, 50, and 80 % respiration inhibition (EC20, EC50, EC80) the nominal content of the test substance was used, assuming the test compound to be stable in water over 3 h.
At the applied test concentrations, the test substance showed no significant toxic effects (<15 %) to the activated sludge. The respiration rates were in the same range as determined for the two blank controls.
Based on these data the EC20, EC50, EC80 values of the test material were estimated to be >2000 mg/l.
Reference
Nominal concentration (mg/l) | Code | O2 consumption rates (mgO2/L h) | Respiration inhibition (%) |
0 | B1 | 85.1 | - |
0 | B2 | 90.4 | - |
125 | T1 | 86.0 | 2.0 |
250 | T2 | 95.0 | -8.3 |
500 | T3 | 83.7 | 4.6 |
1000 | T5 | 88.0 | -0.3 |
2000 | T5 | 77.7 | 11.5 |
Reference 3 mg/l | R1 | 44.3 | 49.5 |
Reference 10 mg/l | R2 | 23.4 | 73.3 |
Reference 30 mg/l | R3 | 8.5 | 90.4 |
Description of key information
The inhibitory effect of p-Nitrobenzoic acid, compound with 2,2',2''-nitrilotriethanol (1:1) (CAS no. 7394-38-9) on the oxygen consumption of activated sludge microorganisms of a municipal sewage treatment plant was investigated under aerobic static conditions. The inhibitory effect may include the effect on respiration and nitrification. The exposure time of the test substance on the activated sludge was 3 hours.
At the applied test concentrations, the test substance showed no significant toxic effects (<15 %) to the activated sludge.
Based on these data the EC20, EC50, EC80 values of p-Nitrobenzoic acid, compound with 2,2',2''-nitrilotriethanol (1:1) were estimated to be >2000 mg/l.
Key value for chemical safety assessment
Additional information
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