Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 226-901-0 | CAS number: 5538-94-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 August 1986 - 18 Spetember 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Remarks:
- Read-across
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian cell transformation assay
Test material
- Reference substance name:
- Reference substance 001
- Cas Number:
- 7173-51-5
- Test material form:
- liquid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- CHO-K1B4
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S9 from Aroclor 1254 induced rats.
- Test concentrations with justification for top dose:
- The concentration of the test substance employed was based on the results from a toxicity test. The levels selected were 2, 4, 8 and 16 µg/ml (with S9 mix) and 1, 2, 4 and 8 µg/ml (without S9 mix).
- Vehicle / solvent:
- Distilled water.
Controls
- Untreated negative controls:
- yes
- Remarks:
- Vehicle control - distilled water
- Negative solvent / vehicle controls:
- no
- True negative controls:
- yes
- Remarks:
- Untreated control cultuure
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- cyclophosphamide
- methylmethanesulfonate
- Details on test system and experimental conditions:
- Mitotic inhibitor was colcemid (N-deacetyl-N-methylcolchicine)
- Evaluation criteria:
- All structural chromosome aberrations were assigned artifical lesion or break scores and the aberrations yield quantified as follows:
i) Chromatid and chromosome (isochromatid) gaps, breaks and acentric fragments were counted as one lesion each, indicating that a single event had generated the damage.
ii) Chromatid and chromosome complexes, such as exchanges, rings and dicentrics were designated 2 lesions each, indicating that 2 separate breaks has occurred. A cell containing a rearrangement and an associated fragment was, however, scored as having 2 and not 3 lesions, due to the likely shared origin of this damage.
iii) Metaphase with chromosomes showing multiple and extensive aberrations were awarded an arbitary value of 10 lesions per cell.
Results and discussion
Test results
- Key result
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The substance was found to be negative for clastogencity in a chromosome aberration test using Chinese hamster ovary cells in the presence and absence of a metabolic activation system.
- Executive summary:
The substance was tested for clastogenic potential using Chinese hamster ovary cells in vitro in the presence and absence of a metabolic activation system (liver S9 from Aroclor 1254 induced rats). There was no evidence of chromosomal aberrations and no indication of chromosomal ploidy changes following exposure to the substance at concentrations of 2, 4, 8 and 16 µg/ml (with S9 mix) and 1, 2, 4 and 8 µg/ml (without S9 mix). It was concluded that the substance was negative for clastogencity in a chromosome aberration test using Chinese hamster ovary cells in the presence and absence of a metabolic activation system.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.