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EC number: 250-701-2 | CAS number: 31565-19-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 July 1985 - 26 July 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- No guideline followed. Analytical verification not fully reliable.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- - Effect concentration based on % water soluble fraction (WSF)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Isooctyl acetate
- EC Number:
- 250-701-2
- EC Name:
- Isooctyl acetate
- Cas Number:
- 31565-19-2
- Molecular formula:
- C10H20O2
- IUPAC Name:
- Acetic acid, isooctyl ester
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from all test concentrations on Day 0, before adding test organisms. Day 1, and on day of termination. Samples were taken from the control, high and low test concentration, as well as the stock solution on Day -1. All BW2 used for test material preparation and all stock solutions were sampled during the study. All analyses were done by the Total Carbon method.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The water soluble fraction of MRD-83-303 was prepared by combining the test material with BW2 at a ratio of approximately 6.7 ml test material:1 liter blend water. The mixture was then stirred for approximately 72 hours prior to use as the 100% stock solution. The water soluble fraction was drawn out of the mixing vessel by way of a sampling tube.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISMS
- Source: Cultured in the Environmental Toxicology Laboratory
- Number: 120
- Age: < 24 hours old
- Cultures of Daphnia were fed Selenastrum capricornutum and a trout chow mixture. The algae were cultured in the Aquatic Laboratory using modified ASM-1 media.
ENVIRONMENTAL CONDITIONS
- Test organisms were not fed during the study
- Daphnids are kept in 1 liter glass culture vessels with dilution water (BW2 at 21 ± 1°C)
ACCLIMATION
Daphnids were kept in 1 liter glass culture vessels with dilution water (BW2) similar to that which was used during the test phase. Daphnids were maintained at test temperature (21 +/- 1 degree C) for at least 48 hours prior to start of test
IN LIFE DATES
24 July 1985 - 26 July 1985
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Remarks:
- Laboratory dilution water (Blend Water 2)
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 153 - 157 mg/L CaCO3
- Test temperature:
- 21.7 - 22.3 C
- pH:
- 7.5 - 8.1
- Dissolved oxygen:
- 7.6 - 7.9 mg/l
- Conductivity:
- 340-355 uhmos
- Nominal and measured concentrations:
- Nominal concentration (%WAF): 6.25, 12.5, 25, 50, 100
Measured concentration (as ppm TC): 1.87, 4.13, 10.24, 20.21, 39.95 - Details on test conditions:
- TEST SYSTEM
- Test vessel: The test chambers were constructed of glass and stainless steel
- Material, size, headspace, fill volume: The test chambers were constructed of glass and stainless steel with an approximate volume of 750 ml.
- Type of flow-through (e.g. peristaltic or proportional diluter): The MRD-83-303 test material water soluble fraction was administered in aqueous solution via a diluter system,
- Renewal rate of test solution (frequency/flow rate): Approximately 6 liters of test solution was delivered to each replicate test chamber per 24 hour period.
OTHER TEST CONDITIONS
- Photoperiod: Diumal light, approximately 16 hours light followed by 8 hours dark with a gradual intensity conversion between periods.
- Light intensity: Daylight intensity ranged from 83 to 87 footcandles as measured by computer IP 31.
RANGE-FINDING STUDY
- Test concentrations: Nominal (% WSF): 0, 6.25, 12.5, 25, 50, 100
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 73.58 other: % WSF
- Details on results:
- Test results were used to derive the 48-h EC50, defined as the calculated concentration of the test material which caused 50% mortality in the population of test organisms at the specified time of exposure. The statistical method used to calculate the LC 50 values was a Maximum Likelihood probit analysis based on D. J. Finney, 1971.
Any other information on results incl. tables
Table 1: Study observation data
|
Table 2: Results of water quality analysis
|
Table 2: Nominal and measured exposure concentrations
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- A 48-h EC50 of 73.58 (%WSF) was determined for Daphnia magna. Based on the 48-h EC50, the test material is not classified as acutely toxic to aquatic invertebrates according to Regulation (EC) No. 1272/2008 (CLP).
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