Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 911-501-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-02-19 to 2007-05-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to OECD guideline
- Justification for type of information:
- See attached justification document
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- The T° for the main study lay in a range of 24.3°C to 26.3°C and therefore higher than stated in the guidelines.As no mortality occured during the test, this deviation was stated as uncritical
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- The T° for the main study lay in a range of 24.3°C to 26.3°C and therefore higher than stated in the guidelines.As no mortality occured during the test, this deviation was stated as uncritical
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 2007-01-12
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- Preparation of the test solution:
Pre-test: A stock solution containing 1000 mg/L test item was prepared. This solution was used to prepare the test solutions: Date: Feb. 19-23, 2007 Replicates one vessel containing 3 L test solution and 3 Fish. Control: one vessel, containing 3L dilution water and 3 fish.
Main study: A stock solution containing 700 mg/L test item was prepared. This solution was used to prepare the test solutions: Date: May. 21-25, 2007 Replicates one vessel containing 7 L test solution and 7 Fish. Control: one vessel, containing 7L dilution water and 7 fish. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Danio Rerio
- Strain: Hamilton- Buchanan
- Source: Weiler's pet's best, Turmstr. 6, 67433 Neustatd
- Age at study initiation (mean and range, SD): sexually immature young fish
- Length at study initiation (length definition, mean, range and SD): 2 ± 1 cm
- Weight at study initiation (mean and range, SD): Not reported
- Feeding during test: No
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions : same as test
- Type and amount of food: warmwater fishfood and daphnia 1-2% of bodyweight per day
- Feeding frequency: 3 times a day
- Health during acclimation (any mortality observed): No data - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 1.08 mmol/L
- Test temperature:
- 24.3 °C to 26.3°C measured (In old solution) at 0h, 24h, 48h, 72h, and 96h
- pH:
- 7.6 to 8.3 measured at 0h, 24h, 48h, 72h, and 96h
- Dissolved oxygen:
- Stay above 6.8 mg/L (range 6.8-8.1 for control, range 7.1-9.0 for exposed)
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- The measured concentration after 96h was 100% and 99% of the nominal concentration and the start concentration, respectively.
(See table measured concentration) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria,
- Material, size, headspace, fill volume: size = 10 L fill volume = 7 L
- Aeration: accomplish with glass tube, frequency of bubble 1/s
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable (static test)
- Renewal rate of test solution: Not applicable (static test)
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): Not applicable
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Good quality drinking water is used
- Total organic carbon: <1.0mg/L
- Particulate matter: No data
- Metals: No data
- Pesticides: No data
- Chlorine free tap-water: Tap water
- Alkalinity: No data
- Ca/mg ratio: No data
- Conductivity: 229µS/cm
- Intervals of water quality measurement: once a year
OTHER TEST CONDITIONS
- Adjustment of pH: No data
- Photoperiod: 12h-12h
- Light intensity: No data
EFFECT PARAMETERS MEASURED: Observations were made every 24h , measuring pH and dissolved oxygen concentration of the old and the new test solution in each vessel, and documenting mortalities or abnormal behaviour.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (Limit test)
- Range finding study: Yes
- Test concentrations: 10 mg/L and 100 mg/L
- Results used to determine the conditions for the definitive study: For each concentration, no fish died or showed any signs of morbidity at the end of the range finding test. The pH and the oxygen value were in the range specified by guidelines - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: None
- Observations on body length and weight: No data reported
- Other biological observations: No
- Mortality of control: No
- Other adverse effects control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Sublethal observations / clinical signs:
Mortalities
Nominal Concentration in mg/L
Total of fish used
Dead fish
24h
48h
72h
96h
New
Cum.
New
Cum.
New
Cum.
New
Cum.
0
7
0
0
0
0
0
0
0
0
100
7
0
0
0
0
0
0
0
0
pH and O2values
Nominal Concentration in mg/L
pH
O2in mg/L
0h
24h
48h
72h
96h
0h
24h
48h
72h
96h
0
8.3
7.7
7.7
7.8
7.8
8.1
6.8
6.9
7.1
7.0
100
7.6
7.7
7.7
7.8
7.7
9.0
7.1
7.1
7.4
7.3
Temperature
0h
24h
48h
72h
96h
24.3
24.6
26.3
25.9
25.6
Measured concentrations
Nominal Concentration in mg/L
Measured concentrations in mg/L
% recovery after 96h
% of nominal concentration
0h
96h
0h
96h
0
<LOQ
<LOQ
100
101.9
100.5
99
102
100
- Validity criteria fulfilled:
- yes
- Conclusions:
- The validity criteria were met.
The analytical determinations of the test item showed very good correlation between nominal and measured concentrations and very good recovery rates. Therefore, the test item can be stated as "stable under the test conditions" and the determination of the results was based on the nominal concentrations.
The result of the test can be considered valid - Executive summary:
One reliable key study is available on freshwater fish (Danio rerio) for the potassium salt of the Isobutyl Acid Phosphate. The reaction mass IBAP and its potassium salt have high water solubility and their behavior in water and biological systems is dominated by their ionization. The main assumption is that the potassium cation is not significant in respect of the toxicological effect on aquatic organisms which are expected to be related to the IBAP. In experimental dilute aqueous conditions at environmental pH (5-9), the salt will be completely dissociated and will not behave differently to the parent acid, at identical concentration of the particular speciated form present.
In a 96h acute toxicity study in accordance with OECD Guideline 203 and under GLP, Danio rerio, were exposed to Potassium isobutylphosphate at a nominal concentration of 100 mg/L (limit test) under static conditions at 23 +/- 1°C. The mortality was observed daily and the pH, the concentration of dissolved oxygen and the concentrations of the test item were measured at the beginning and the end of the test. The analysis of the test item concentration was performed on phosphate using a validated photometer method.
No mortality was observed in the treated groups and the validity criteria's were fulfilled. The 96h LC50was determined above 100 mg/L and the 96h NOEC above or equal 100 mg/L.The results on effects are expressed in nominal concentration of test item because the analytical measurement based on phosphate show that it was stable among the study (99% recovery after 96h).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.