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EC number: 203-385-5 | CAS number: 106-32-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-01-29 to 2002-01-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- DOC was measured in all concentration levels and control at test start (0 h, fresh media) and test end (48 h, old media).
- Details on test solutions:
- - Stock solution: 100 mg/L was prepared with dilution water one day prior to application
- Dispersion treatment: 24 h an a magnetic stirrer, afterwards filtration with 0.45 µm
- Test dilutions: 1:16-1:8-1:4 -1:2-1:1
- Real test concentrations: 0.6 - 1.1 -2.5-4.8 - 9.3 mg/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: Institut für Wasser-, Boden- und Lufthygiene des Bundes- gesundheitsamtes, Corrensplatz, D-14195 Berlin
- Feeding: 5 x weekly ad libitum with a mix of Scenedesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 106cells/mL
- Culture medium: Elendt M4, according to Elendt (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Culture: In 2-3 L glass vessels with approximatively 1.8 L culture medium, at 21 °C, in an incubator, 16 h illumination, illumination strength max. 20 µmol-m-2.s-1.
- Age: less than 24h old
- Acclimation: 2h in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 265 mg/L
- Test temperature:
- 18 - 22 +/- 1 °C
- pH:
- 7.72
- Dissolved oxygen:
- 8.62 mg/L O2
- Nominal and measured concentrations:
- Measured concentrations (Based on DOC analysis): 0, 0.6, 1.1, 2.5, 4.8 and 9.3 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H), 50 mL
- Volume study medium: 20 mL
- Number of study organisms per concentration and control: 20 animals
- Number of animals per test vessel: 5
- Number of parallel samples per concentration: 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to EEC 92/69 L383A C.2
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0 h; 48 h (pH, dissolved oxygen); temperature permanently
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark
- Illumination: Diffuse light, illumination strength max. 20 µmol-m-2 (corresponding to 1200 lx)
TEST CONCENTRATIONS
- Test concentration: 5 dilutions: 0.6 - 1.1 -2.5-4.8 - 9.3 mg/L
CONTROL
- dilution water - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: DOC concentrations
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: DOC concentrations
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 9.3 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: DOC concentrations
- Basis for effect:
- mobility
- Details on results:
- The test item was clearly dissolved in all concentration levels throughout exposure.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels after 24h: EC 50 = 2.0 mg/L (CL 1.9 - 2.1 mg/L), EC 10 = 1.7 mg/L
The result is within the prescribed concentration range. - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48h) was determined to be 7.9 mg/L, the EC10 (48h) was 5.4 mg/L and the EC100 (48h) > 9.3 mg/L.
- Executive summary:
In the current study the acute immobilisation (EC50) of the test item to Daphnia magna (Straus) was determined according to OECD guideline 202.
The study was conducted under static conditions over a duration of 48 hours. 20 test organisms were exposed to each test concentration and control. The real test concentrations were calculated based an the DOC-analysis of the test item dilutions alter 0 and 48 h (mean values of DOC measurments): 0.6, 1.1, 2.5, 4.8 and 9.3 mg/L
The test item was clearly dissolved in all concentration levels throughout exposure. The 24 h and 48 h EC10 and EC50 values were calculated by probit analysis in the tested concentration range.
The EC50 (48h) was determined to be 7.9 mg/L, the EC10 (48h) was 5.4 mg/L and the EC100 (48h) > 9.3 mg/L.
Reference
Range finding test results
Mean immobilisation rates (%) after 24 and 48h:
Dilution | 24h | 48h |
1:1 | 95 | 100 |
1:10 | 0 | 0 |
1:100 | 0 | 0 |
Control | 0 | 0 |
Results of the main test
Mean immobilisation rates (%) after 24 and 48h:
Concentration (mg/L) | 24h | 48h |
9.3 | 65 | 70 |
4.8 | 0 | 0 |
2.5 | 0 | 0 |
1.1 | 0 | 0 |
0.60 | 0 | 0 |
Control | 0 | 0 |
DOC - Analysis of New (Oh) and OId Media (48h)
Dilution |
DOC (mg/L) |
ThTOC (mg/L) |
Recovery rate (%) |
Real concentration (mg/L) |
|||
0 h |
48 h |
mean |
Corrected against DOC control |
||||
1:1 |
11.33 |
9.44 |
10.39 |
6.48 |
69.71 |
9.3 |
9.3 |
1:2 |
7.44 |
7.11 |
7.28 |
3.37 |
34.86 |
9.7 |
4.8 |
1:4 |
5.64 |
5.60 |
5.62 |
1.71 |
17.43 |
9.8 |
2.5 |
1:8 |
4.77 |
4.59 |
4.68 |
0.77 |
8.71 |
8.8 |
1.1 |
1:16 |
4.29 |
4.38 |
4.34 |
0.43 |
4.36 |
9.9 |
0.6 |
Control |
4.22 |
3.59 |
3.91 |
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Description of key information
EC50 (48 h) = 7.9 mg/L (Daphnia magna, OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 7.9 mg/L
Additional information
One experimental study is available investigating the short-term effects of the substance to aquatic invertebrates. The study was performed according to OECD 202 (GLP) with the water flea Daphnia magna. Measured concentrations of 0.6, 1.1, 2.5, 4.8 and 9.3 mg/L were tested under static conditions. Test concentrations were verified by DOC-analysis at the beginning and end of the exposure period. Immobilisation of D. magna was observed after 48 h resulting in an EC50 (48 h) of 7.9 mg/L based on the arithmetic mean measured concentrations.
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