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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 04, 2017 to November 08, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- see 'Principles of method if other than guideline'
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- yes
- Remarks:
- see 'Principles of method if other than guideline'
- Principles of method if other than guideline:
- The following deviation from the guideline was documented:
- Temperature range was 19.4 – 21.5°C instead of 20.0 – 24.0 °C. As degradation of the positive control was in the normal range this is considered as not critical to the outcome of the study. - GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- CAS No.: 162492-01-5 (or 264888-31-5); EINECS-No.: 500-734-6; Batch No.: DR0072912; Appearance: transparent viscous liquid, Purity: 100 % (UVCB); Homogeneity: homogeneous
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Specification:
Activated sludge from a biologic sewage treatment plant was used as inoculum. The chosen plant is treating mostly domestic sewage.
Source:
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Pre-Treatment:
The sludge was filtrated, washed with tap water (2x), then washed with and re-suspended in test medium. It was then aerated until use. The dry matter was determined as 4520 mg suspended solids/L. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 35.5 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Preparations:
The medium was prepared from the stock solutions. The stock solution of the positive control was prepared and its DOC was measured. The inoculum was taken from its source, washed, aerated and the dry matter was determined.
The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2.
On the day of the start of the test, CO2-free medium and inoculum was filled into the test flask.
Experimental Parameters:
Flask volume 1500 mL
Apparatus blanks: 2, containing mineral medium only
Blank Controls: 2, containing mineral medium and inoculum
Positive control flasks: 2, containing positive control, mineral medium and inoculum
Test flasks: 2, containing test substance, mineral medium and inoculum
Abiotic control: 1, containing test substance, mineral medium and HgCl2
Toxicity control: 1, containing test substance, positive control, mineral medium and inoculum
Inoculum concentration: 25.0 mg/L
Temperature: 19.4 – 21.5 °C
Duration: 28 days
The test was performed with a nominal start concentration of 20 mg organic carbon/L.
Apparatus:
The test vessels were aerated with purified (by activated charcoal), CO2-scrubbed, moistened air. The scrubbing of carbon dioxide was achieved by bubbling the purified air through a flask containing 1.5 M NaOH. To control the absence of CO2, the air was then led through a flask containing a solution of Ba(OH)2 before reaching the test vessels.
Magnetic stirrers were used to prevent deposition of inoculum.
The emitted CO2 was trapped in 0.25 M NaOH. Two scrubbers containing 100 mL each were connected in series to the test vessels. The initial IC value of the 0.25 M NaOH was separately determined in each flask.
Sampling:
From each front scrubber flask, 9 samples were taken in order to determine the emitted CO2 (on day 0, 2, 4, 7, 10, 14, 18, 24 and 29). The sample volume was 1 mL. The resulting change in the volume of the front flask was considered in the calculation of emitted CO2.
On day 28, 5 mL HCl 2 M was added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken. - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 5
- Sampling time:
- 28 d
- Details on results:
- All validity criteria were met.
- Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation reached 1.5 %. Both replicates of the test substance showed very good correspondence.
- If degradation in the toxicity flask is below 25 % after 14 days, the test substance can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 31.3 % after 14 days, the test substance can be stated as “not toxic towards the inoculum in a concentration of 35.5 mg/L”. - Results with reference substance:
- 69 % after 10 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the study conditions, the degradation of the test substance did not reach the pass level of 60% in the course of the test, therefore the test substance is considered as not readily biodegradable.
- Executive summary:
A study was conducted to determine the ready biodegradability of the test substance according to OECD Guideline 301B and EU Method C.4 – C (CO2 evolution test), in compliance with GLP. The test substance was tested at a nominal concntration of 20 mg organic carbon/L (corresponding to 35.5 mg test substance/L) in test medium. Activated sludge was used as inoculum and the test substance in a mineral medium was incubated under aerobic conditions in the dark through 28 d. The amount of DOC in the test solution due to the inoculum was kept as low as possible compared to the amount of organic carbon due to the test substance. Degradation was followed by determination of the carbon dioxide produced with a TOC analyser. All validity criteria were met. Degradation of the positive control aniline was 69% after 10 d. The degree of biodegradation of the test substance reached 5% after 28 d and therefore, the degradation did not reach the pass level of 60% in the course of the study. Under the study conditions, the test substance is considered as not readily biodegradable (Muckle, 2018).
Reference
Validity:
Parameter |
Criterion |
Found |
Assessment |
IC content of test substance solution in medium |
≤5% of TC |
0 % |
valid |
CO2emitted by the controls |
< 70 mg/L |
9.5 mg/L |
valid |
Difference within replicates |
≤20% |
2.6 % |
valid |
Degradation of positive control > 60% |
≤14 days |
10 days |
valid |
Degradation in the toxicity flask on day 14 |
> 25% |
31.3 % |
valid |
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
A study was conducted to determine the ready biodegradability of the test substance according to OECD Guideline 301B and EU Method C.4 – C (CO2 evolution test), in compliance with GLP. The test substance was tested at a nominal concntration of 20 mg organic carbon/L (corresponding to 35.5 mg test substance/L) in test medium. Activated sludge was used as inoculum and the test substance in a mineral medium was incubated under aerobic conditions in the dark through 28 d. The amount of DOC in the test solution due to the inoculum was kept as low as possible compared to the amount of organic carbon due to the test substance. Degradation was followed by determination of the carbon dioxide produced with a TOC analyser. All validity criteria were met. Degradation of the positive control aniline was 69% after 10 d. The degree of biodegradation of the test substance reached 5% after 28 d and therefore, the degradation did not reach the pass level of 60% in the course of the study. Under the study conditions, the test substance is considered as not readily biodegradable (Muckle, 2018).
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