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EC number: 205-265-8 | CAS number: 136-85-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 05 November 2019 to 07 November 2019
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- resp. EU C.2
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- GLP certificate is included in the Study Report.
Test material
- Reference substance name:
- 6-methylbenzotriazole
- EC Number:
- 205-265-8
- EC Name:
- 6-methylbenzotriazole
- Cas Number:
- 136-85-6
- Molecular formula:
- C7H7N3
- IUPAC Name:
- 6-methylbenzotriazole
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Batch number: N6A3I
Expiry date: 04 August 2022
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Berlin
- Food type: green algae (Desmodesmus subspicatus)
22 hours and 25 minutes before the start of the test, the adult animals were separated from the young. 0.5 hours before test start, the adults were caught with the help of a glass tube, and the newborn daphnia (age < 24h) were sieved from the medium and immediate-ly placed into a beaker containing dilution water. After the settling-in period, animals which showed no apparent damage were used for the test.
Switching from M4-medium (husbandry) to Dilution water (test) has been shown not to cause any detrimental effects for test daphnia, therefore no acclimation period of 48 h was performed. This is a deviation from OECD Guideline No. 202 and EU-Method C.2, which can be considered uncritical
Study design
- Test type:
- not specified
- Water media type:
- other: Dilution water (Daphnia medium) with the following specification was used : CaCl2*2H2O 293.80 mg/L; MgSO4*7H2O 123.30 mg/L; NaHCO3 64.80 mg/L; KCl 5.80 mg/L
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 19.3 - 20.4 °C
- pH:
- 7.4 - 7.8
- Dissolved oxygen:
- 8.5 - 8.8
- Nominal and measured concentrations:
- Nominal concentrations (mg/L):4.6; 10; 22; 46; 100
mesaured concentrations (mg/L, t=0h): 4.73; 10.42; 21.85; 44.86; 97.43
mesaured concentrations (mg/L, t=48h):5.18; 11.49; 24.31; 53.67; 111.22
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 46 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 22 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 51.57 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 67.63 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L nominal concentration. After 48 hours the 2 highest concentrations 46 mg/L and 100 mg/L showed toxicity between 20 and 100% immobilisation. None of the animals was immobilised in the blank control and the lower concentrations.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.
The analytical determinations of the test item showed good correlation between nominal and measured concentrations and good recovery rates. The measured concentrations at the beginning and at the end lay between 102 % and 105 % of the nominal concentra-tions.
Therefore, the determination of the results was based on the nominal concentrations (see Guidance Doc. No.23 (§176)).
No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.
The result of the test is considered valid. - Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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