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EC number: 235-406-9 | CAS number: 12220-06-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From April 06th to May 19th, 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Part I, May 12, 1981, adopted 1984
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was dissolved in the test medium at a concentration of 100 mg/l (stock solution). Thereafter, series of sequential dilutions with the test medium were prepared to obtain the final test concentrations of the test article.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Breeding: bred in the testing laboratories, under standardized conditions.
- Age at study initiation: age of less than 24 hours. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Test temperature:
- 21.5 °C
- pH:
- Start: 8.3 - 8.4
End: 8.3 - 8.4 - Dissolved oxygen:
- Start: 8.4 - 8.8 mg/l
End: 8.6 - 9.1 mg/l - Nominal and measured concentrations:
- 1, 10 and 100 mg/l nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beakers, containing 20 ml of test medium.
- Aeration: test medium was aerated to an oxygen concentration of 8 mg/l.
- No. of organisms per vessel: 10 animal.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 2 replicates.
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to the OECD guideline. The water used was bidlstilled water.
- Total hardness: 14 dH°.
- CaCl2 x 2H2O: 294 mg/l
- MgSO4 x 7H2O: 123 mg/l
- NaHCO3: 65 rng/l
- KCl: 5.8 mg/l
OTHER TEST CONDITIONS
- Adjustment of pH: the pH of the reconstituted water was abjusted to 8.2.
- Photoperiod: 16 hours of illumination.
- Light intensity: 1000 Lux.
EFFECT PARAMETERS MEASURED
Test evaluations were done according to the OECD guideline 202 by visual control of the mobility of the daphnids after 24 hours. Basect on the data, an estimate of the EC0, 50 and 100 was given. - Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The EC0 was identified in the range of 1 - 10 mg/l
- Validity criteria fulfilled:
- yes
- Remarks:
- in the control, not more than 10 % of the Daphnia have been immobilized or trapped at the surface of the water and dissolved oxygen concentration was ≥ 60 % of the air saturation
- Conclusions:
- EC50 (24h) > 100 mg/l (nominal)
- Executive summary:
The acute toxicity of the test substance to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202. The nominal test concentrations applied were 1, 10 and 100 mg/l. No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 1 mg/l; at the nominal concentrations of 10 and 100 mg/l 10 % of total fish died after 24 hours of exposure. 10 % of mortality was also observed in one of the two the blank controls.
The EC0 was identified to be in the range of 1 - 10 mg/l.
Conclusion
EC50 (24h) > 100 mg/l (nominal)
Reference
Immobilization of Daphnia magna
Conc. Of test article (mg/l) | N. of animals | N. of responses 24 hrs | % of responses 24 hrs |
0 | 10 | 1 | 10 |
0 | 10 | 0 | 0 |
1 | 10 | 0 | 0 |
1 | 10 | 0 | 0 |
10 | 10 | 0 | 0 |
10 | 10 | 1 | 10 |
100 | 10 | 1 | 10 |
100 | 10 | 1 | 10 |
Description of key information
Not short-term harmful/toxic to aquatic invertebrates.
Key value for chemical safety assessment
Additional information
The acute toxicity of the test substance to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202. The nominal test concentrations applied were 1, 10 and 100 mg/l. No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 1 mg/l; at the nominal concentrations of 10 and 100 mg/l 10 % of total fish died after 24 hours of exposure. 10 % of mortality was also observed in one of the two the blank controls. The EC0 was identified to be in the range of 1 - 10 mg/l, while the EC50 (24h) value was indicated to be higher than 100 mg/l (nominal).
A second experiment is available. It was performed on Daphnia magna, over a period of 48 hours and following the OECD guideline 202. The following nominal test concentrations were chosen and tested in duplicate: 0.9, 1.9, 4.3, 9.4, 21 and 45 mg/l. No chemical analysis of the test substance concentrations was performed. In this case test, weak sedimentation was observed after 48 hours at the concentration of 21 mg/l; at 45 mg/l moderate sedimentation was observed at 24 and 48 hours. The EC50 (48h) was indicated to be 31 mg/l (nominal, geom mean).
The second experiment performed on daphnia has been judged as not reliable due to sedimentation recorded; in fact, effects were recorded at concentrations at which weak/moderate sedimentation occurred. Thus, the test results could be negatively impacted due to physical effects. Sedimentation is consistent with the substance behaviour observed in the Lemna experiment: the medium used is similar to that used in the daphnia experiment. The preparation of stock solution for the Lemna test resulted in a dense, dark orange suspension that was then filtrated to separate the possible non-dissolved test material. During filtration strong coloration of the membrane was observed due to undissolved test item residue, resulting in a light orange test item stock solution. The test solutions of the subsequent lower test concentrations were prepared. The highest concentration reachable was 0.69 mg/l.
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