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EC number: 272-559-0 | CAS number: 68877-33-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Version / remarks:
- 2006
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- Preparation of stock and test solutions: A stock solution has been prepared with a concentration of 1.0 g/L of the material presented (97.54% active matter). Therefore, 0.1000 g of the material has been dissolved in 100 mL of culture medium in a volumetric flask, Class A of 100 mL by shaking, so we have a stock solution of 975.4 mg/L of active matter . The different solutions used in the test have been prepared from this stock solution of 975.4 mg/L of active material of the test product.
Concentrations tested (active matter): 0.98 mg/L, 3.13 mg/L, 9.78 mg/L, 31.3 mg/L, 97.8 mg/L, 312.9 mg/L and 977.7 mg/L
- Actual concentration of the test stock solution (active matter): 977.7 mg/L - Test organisms (species):
- Lemna minor
- Details on test organisms:
- - Source : Federal Enviromental Agency (Berlín, Germany)
Growing conditions:
o Temperature: 24 ± 2ºC
o Intensity and quality of lighting: 6500 Lux - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Test temperature:
- 24±2ºC
- pH:
- See pH value during the test (Table 1) in section "Any other information on materials and methods incl. tables"
- Nominal and measured concentrations:
- Concentrations tested: 1, 3,2, 10, 32, 100, 320 y 1000 mg/L
Concentrations tested (active matter): 0.98 mg/L, 3.13 mg/L, 9.78 mg/L, 31.3 mg/L, 97.8 mg/L, 312.9 mg/L and 977.7 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 ml beaker
- Material, size, headspace, fill volume: glass with 100 ml of test medium
GROWTH MEDIUM
- Standard medium used: yes
Test medium with the following composition:
Macroelements
— Solution 1: KNO3 17,5 g/L H2O; KH2PO4 4,5 g/L H2O; K2HPO4 0,63 g/L H2O
— Solution 2: MgSO4 7H2O 5 g/L H2O
— Solution 3: Ca(NO3)2 4H2O 14,75 g/L H2O
Microelements
— Solution 4: H3BO3 120mg/L H2O
— Solution 5: ZnSO4 7H2O 180 mg/L H2O
— Solution 6: Na2MoO4 2H2O 44,0 mg/L H2O
— Solution 7: MnCl2 4H2O 180,0 mg/L H2O
— Solution 8: FeCl3 6H2O 760,0 mg/L H2O
AEDT Na2 2H2O 1500,0 mg/L H2O
Add to 1000 mL of deionized sterile water:
Solution 1 20 mL
Solution 2 20 mL
Solution 3 20 mL
Solution 4 1 mL
Solution 5 1 mL
Solution 6 1 mL
Solution 7 1 mL
Solution 8 1 mL
- Number of replicates per trial: 3
- Number of test concentrations: 7 + control
- Geometric progression factor used: 3.2
ILLUMINATION:
Culture apparatus: Incubation chamber
Intensity and Quality of light: 6500 Lux
The surface where the test is performed receives uniform fluorescent lighting and continuous (white-cold type). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-diclofenol
- Key result
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 103 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Key result
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 575 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Details on results:
- The validation criterion considered for this study is:
- The average number of fronds in the control must present a growth rate specific medium of at least 0.275 per day.
- Duplication time of approximately 2.5 days, and multiplication by 7 of the number of fronds, at the end of the study
- The EC50 7d value of 3,5-diclofenol is in the range 2.2 - 3.8 mg/L. - Results with reference substance (positive control):
- The reference substance used to verify the response of the test organisms is 3,5-diclofenol. The result of the periodic verification of the response of the test organisms is EC50 7d = 2.9 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The concentration of the test item that produces an inhibition of the 50% growth rate at 7 days (EC50 7d) in Lemna minor is 575 mg/L.
The concentration of the test item that produces an inhibition of Biomass of 50% at 7 days (EC50 7d) in Lemna minor is 103 mg/L. - Executive summary:
The study of acute toxicity to aquatic plants of Direct Black RBB dye was carried out. For the determination of the inhibition of algal growth, the freshwater aquatic plant Lemna minor has been used and the operative method according to the OECD Guideline 221 (2006) has been followed.
The freshwater aquatic plant Lemna minor has been exposed to the substance at the test concentrations 1; 3,2; 10; 32; 100; 320 and 1000 mg/L based on active material. The total duration of the trial has been 7 days.
The result obtained in this test indicates that the dye Direct Black RBB dye presents an inhibition value of the EC50 growth rate (7d) equal to 575 mg/L and an inhibition value of the biomass EC50 (7d) equal to 103 mg/L after 7 days of the test.
Reference
Growth rate inhibition: The results of the count of fronds at the beginning and at the end of the test are represented in the following table (table 2 of the study report)
Table 2: Number of fronds
Replicas Nº of fronds | control | Concentration (mg/L) | ||||||||||||||
0,98 | 3,13 | 9,78 | 31,3 | 97,8 | 312,9 | 977,7 | ||||||||||
0d | 7d | 0d | 7d | 0d | 7d | 0d | 7d | 0d | 7d | 0d | 7d | 0d | 7d | 0d | 7d | |
1 | 12 | 86 | 12 | 80 | 12 | 72 | 12 | 57 | 12 | 52 | 12 | 38 | 12 | 35 | 12 | 31 |
2 | 12 | 87 | 12 | 77 | 12 | 74 | 12 | 54 | 12 | 51 | 12 | 39 | 12 | 36 | 12 | 29 |
3 | 12 | 81 | 12 | 79 | 12 | 70 | 12 | 57 | 12 | 47 | 12 | 43 | 12 | 38 | 12 | 28 |
4 | 12 | 92 | 12 |
| 12 |
| 12 |
| 12 |
| 12 |
| 12 |
| 12 |
|
5 | 12 | 82 | 12 |
| 12 |
| 12 |
| 12 |
| 12 |
| 12 |
| 12 |
|
6 | 12 | 88 | 12 |
| 12 |
| 12 |
| 12 |
| 12 |
| 12 |
| 12 |
|
Average | 12 | 86 | 12 | 78,7 | 12 | 72 | 12 | 56 | 12 | 50 | 12 | 40 | 12 | 36,3 | 12 | 29,3 |
Sd | 0 | 4,0 | 0 | 1,5 | 0 | 2,0 | 0 | 1,7 | 0 | 2,6 | 0 | 2,6 | 0 | 1,5 | 0 | 1,5 |
CV | 0 | 4,7 | 0 | 1,9 | 0 | 2,8 | 0 | 3,1 | 0 | 5,3 | 0 | 6,6 | 0 | 4,2 | 0 | 5,2 |
From the difference in the number of fronds at the beginning and at the end of the trial, the growth rates for the control and for each of the concentrations are calculated. The growth rates at 7 days are represented in the following table:
Table 3: Growth rate
Growth rate |
| Concentration (mg/L) | ||||||
control | 0,98 | 3,13 | 9,78 | 31,3 | 97,8 | 312,9 | 977,7 | |
0.281 | 0.269 | 0.256 | 0.220 | 0.204 | 0.172 | 0.158 | 0.128 |
In the following table, the percentage of inhibition of the growth rate is represented:
Table 4: % Inhibition of growth rate
Concentration (mg/L) | % inhibition of growth rate |
control | 0 |
0,98 | 4,5 |
3,13 | 9,0 |
9,78 | 21,8 |
31,3 | 27,5 |
97,7 | 38,9 |
312,9 | 43,7 |
977,7 | 54,6 |
Yield inhibition. The weight of the fronds is determined at the beginning and at the end of the test. The initial weight is calculated from the average of 6 repetitions of weighing 12 fronds. The following table shows the weights obtained for each of the test concentrations.
Table 5: Biomass
Concentration (mg/L) | Initial biomass (0days) (mg) | Final biomass (7days)(mg) | Difference of biomass (Bf-Bi) (mg) |
control | 2,0 | 7,9 | 5,9 |
0,98 | 2,0 | 7,4 | 5,4 |
3,13 | 2,0 | 6,9 | 4,9 |
9,78 | 2,0 | 6,4 | 4,4 |
31,3 | 2,0 | 5,7 | 3,7 |
97,7 | 2,0 | 4,6 | 2,6 |
312,9 | 2,0 | 4,3 | 2,3 |
977,7 | 2,0 | 3,9 | 1,9 |
In the following table, the percentage of inhibition of biomass (yield) is represented.
Table 6:% Inhibition of the growth rate
Concentration (mg/L) | % inhibition of biomass growth (yield) |
control | 0 |
0,98 | 8,5 |
3,13 | 16,9 |
9,78 | 25,4 |
31,3 | 37,3 |
97,7 | 55,9 |
312,9 | 61 |
977,7 | 67,8 |
Description of key information
There is one study conducted on the substance Direct Black RBB (CAS 68877-33-8) according to the OECD Guideline 221 to determine the toxicity to aquatic plants Lemna minor. Based on the ECHA guidances, this test method is considered more suitable for testing toxicity of coloured test materials to aquatic plants, than the OECD guideline 201 to test the toxicity to aquatic algae.
The EC50 (7d) value 575 mg/L active ingr. obtained in this study, shows that the tested substance is not harmful to this type of aquatic plant.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 575 mg/L
Additional information
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