Bis(acetato-O)hydroxyaluminium

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-08-29 to 2018-08-31

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

For determination of the test item concentrations samples were taken from each test item concentration and from the control at the start and at the end of the test. One sample was analysed at the start and four replicate samples were analysed at the end of the test of the saturated test solution (each 10 mL). One sample was taken from the control solution at the start and at the end of the test (each 10 mL).

Vehicle:

no

Details on test solutions:

The test solutions used in the test was prepared by mechanical dispersion without using any solubilising agents.
The test item is a poorly water soluble material. For preparation of the test solutions a supersaturated solution (100 mg/L nominal loading) was first prepared by dispersing/dissolving an amount of 0.04 g test item was dissolved in 400 mL dilution water (ISO Medium). This solution was agitated by orbital shaker overnight and then the non-dissolved test material was separated by filtration through a membrane filter (0.22 µm) in order to obtain the saturated test solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Source: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection) 2100 Gödöllő, Kotlán S. u. 3. Hungary
- Age of parental stock (mean and range, SD): Less than 24 hours old
- Feeding during test: no

ACCLIMATION
- Acclimation period: no
- Acclimation conditions: same as test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

approximate 249 mg/L

Test temperature:

20.1 – 20.4 °C

pH:

6.03 – 8.13

Dissolved oxygen:

7.52 – 8.03 mg/L

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

nominal concentrations: 100 % v/v saturated solution
geometric mean concentrations: 0.96 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- Medium volume per beaker: at least 40 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h darkness
- Light intensity: 615 lux

RANGE-FINDING STUDY
- Test concentrations: 100 mg/L (plus control)
- Results used to determine the conditions for the definitive study: no immobilisation at 100 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate, experimantal phase: 07 – 08 March 2017

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.96 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.86 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: calculated value for anhydrous substance

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.96 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.86 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: calculated value for anhydrous substance

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 0.96 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 0.86 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: calculated value for anhydrous substance

Basis for effect:

mortality

Details on results:

There was no immobilisation observed in twenty daphnids exposed to either test item treated or control group. In addition to immobility, no abnormal behaviour or appearance of test animals was detected.

Results with reference substance (positive control):

24h EC50: 1.41 mg/L (95 % confidence limits: 1.19 – 1.68 mg/L)

Validity criteria fulfilled:

yes

Conclusions:

In this 48-hour static acute toxicity limit test with Daphnia magna the effects of Aluminium hydroxide diacetate hydrate on the mobility was determined. The 48-h EC50 value was determined to be >0.96 mg/L (hydrated substance) and >0.86 mg/L (calculated for anhydrous substance). The 48-h NOEC was determined to be 0.96 mg/L (hydrated substance) and 0.86 mg/L (calculated for anhydrous substance).

Executive summary:

The purpose of this study was to evaluate the influence of the test item Aluminium hydroxide diacetate hydrate on the mobility, respectively survival of Daphnia magna in a static test system. Young Daphnia were exposed in an immobilisation test to aqueous test media containing the test item at 100 % v/v saturated solution (corresponding to 0.96 mg/L (calculated geometric mean measured concentrations)). Twenty Daphnia, divided into four groups of five animals each were exposed to the test item concentration or run as control for 48 hours. The 48-h EC50 value was determined to be >0.96 mg/L (hydrated substance) and >0.86 mg/L (calculated for anhydrous substance). The 48-h NOEC was determined to be 0.96 mg/L (hydrated substance) and  0.86 mg/L (calculated for anhydrous substance).

Description of key information

In this 48-hour static acute toxicity limit test with Daphnia magna the effects of Aluminium hydroxide diacetate hydrate on the mobility was determined. The 48-h EC50 value was determined to be >0.96 mg/L (hydrated substance) and >0.86 mg/L (calculated for anhydrous substance). The 48-h NOEC was determined to be 0.96 mg/L (hydrated substance) and 0.86 mg/L (calculated for anhydrous substance).

Additional information

In the acute immobilisation test (reference 6.1.3-1) with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD Guideline for testing of chemicals, Guideline No. 202, adopted 13th April 2004. The study was conducted in a static system as a limit test over a period of 48 hours with a nominal concentrations of 100 % v/v saturated solution (corresponding to geometric measured concentrations of 0.96 mg/L. The validity criteria of the test guideline were fulfilled. The 48-h EC50 value was determined to be >0.96 mg/L (hydrated substance) and >0.86 mg/L (calculated for anhydrous substance). The 48-h NOEC was determined to be 0.96 mg/L (hydrated substance) and  0.86 mg/L (calculated for anhydrous substance).