Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-897-7 | CAS number: 3271-76-9
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977/78
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Federal Register 38 No 187 § 1500.42, S. 27019 dated 27.09.73. Evaluation accordig to Draize, J.H. (1959): Dermal Toxicity. In: FDA-Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics
- Principles of method if other than guideline:
- BASF internal testing method
- GLP compliance:
- no
Test material
- Reference substance name:
- Anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- EC Number:
- 221-897-7
- EC Name:
- Anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- Cas Number:
- 3271-76-9
- Molecular formula:
- C31H15NO3
- IUPAC Name:
- anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- Test material form:
- liquid
- Details on test material:
- Vat Green 3
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- not specified
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not specified
- Amount / concentration applied:
- 0.1 mL
- Observation period (in vivo):
- 24, 48, 72 hours
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- SCORING SYSTEM:
cornea opacity and chemosis
1 - questionable, very mild
2 - mild
3 - severe
4 - most intensive
iris
1 - ciliary injection
2 - iritis
redness
1 - mild
2 - obvious
3 - severe
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.39
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No indication of eye irritation was observed in rabbit eyes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
