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EC number: 220-971-6 | CAS number: 2950-43-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- MUTAGENICITY AND DNA- MODIFYING ACTIVITY: A COMPARISON OF TWO MICROBIAL ASSAYS
- Author:
- HERBERT S. ROSENKRANZ, BEZALEL GUTTER and WILLIAM T. SPECK
- Year:
- 1 976
- Bibliographic source:
- Mutation Research, 41 (1976) 61—70
- Reference Type:
- publication
- Title:
- Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity test
- Author:
- Ames BN, McCann J, Yamasaki E
- Year:
- 1 975
- Bibliographic source:
- Mutation Research, 31, 347-364
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of Hydroxylamine-O-sulfonic acid in Salmonella typhimurium strains TAI535 and TAI538 by using Standard plate incorporation assay
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Hydroxylamine-O-sulphonic acid
- EC Number:
- 220-971-6
- EC Name:
- Hydroxylamine-O-sulphonic acid
- Cas Number:
- 2950-43-8
- Molecular formula:
- H3NO4S
- IUPAC Name:
- Hydroxylamine-O-sulphonic acid
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material (IUPAC name): Hydroxylamine-O-sulphonic acid
- Molecular formula: H3NO4S
- Molecular weight: 113.093 g/mol
- Smiles notation: O=S(=O)(ON)O
- InChl: 1S/H3NO4S/c1-5-6(2,3)4/h1H2,(H,2,3,4)
- Substance type: Organic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): Hydroxylamine-O-sulphonic acid
- Molecular formula: H3NO4S
- Molecular weight: 113.093 g/mol
- Smiles notation: O=S(=O)(ON)O
- InChl: 1S/H3NO4S/c1-5-6(2,3)4/h1H2,(H,2,3,4)
- Substance type: Organic
- Physical state: Solid
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TAI535 and TAI538
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix - liver homogenates(S-9) (9000 g supernatant) from rats induced with a polychlorinated biphenyI (PCB) mixture (Aroclor 1254)
- Test concentrations with justification for top dose:
- 0.1µl
- Vehicle / solvent:
- Not specified
Controls
- Untreated negative controls:
- yes
- Remarks:
- Water
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: Standard plate incorporation assay
DURATION
- Exposure duration: 48 hour - Rationale for test conditions:
- Not specified
- Evaluation criteria:
- Numbers of Histidine revertants per plates were observed.
- Statistics:
- Yes, Mean was observed
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: TAI535 and TAI538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: No mutagenic effect were observed
Any other information on results incl. tables
|
Agent |
Amount |
Revertants per plate |
|
|
|
|
TA1535 |
TA1538 |
Test chemical |
Hydroxylamine-o-sulfonic acid (HOMS) |
0.1µl |
44 |
16 |
Positive control |
Methyl methanesulfonate |
10 µl |
439 |
30 |
Negative Control |
water |
10 µl |
13 |
10 |
Applicant's summary and conclusion
- Conclusions:
- Hydroxylamine-O-sulfonic acid was evaluated for its mutagenic potential in Salmonella typhimurium strains TAI535 and TAI538 by using Standard plate incorporation assay. The test result was considered to be negative in the presence and absence of S9.
- Executive summary:
Genetic toxicity in vitro study of Hydroxylamine-O-sulfonic acid was assessed for its possible mutagenic potential. For this purpose is AMES assay was performed in Salmonella typhimurium strains TAI535 and TAI538 by usingStandard plate incorporation assay. The test chemical was used at the concentration of 0.1µl/plate in the presence and absence of S9 mix. No mutagenic effects were observed. Therefore Hydroxylamine-O-sulfonic acid was considered to be non mutagenic in Salmonella typhimurium strains TAI535 and TAI538 with and without metabolic activation. Hence the substance cannot be classified as gene mutant in vitro.
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