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EC number: 236-599-2 | CAS number: 13446-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-07-05 to 1990-08-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- adopted 24 February 1987
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- The study report states that the study was conducted in compliance with Good Laboratory Practice Standards, e.g. by the United Kingdom Compliance Programme, Department of Health & Social Security 1986 and subsequent revision, Department of Health, 1989.
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Diammonium dimolybdate
- EC Number:
- 248-517-2
- EC Name:
- Diammonium dimolybdate
- Cas Number:
- 27546-07-2
- IUPAC Name:
- Diammonium dimolybdate
- Details on test material:
- - Name of test material (as cited in study report): ammonium molybdate (There was a report amendment which stated that the ammonium molybdate tested in this study was in the form of ammonium dimolybdate).
- Physical state: white crystalline powder
- Analytical purity: > 99.9 %, calculated from reported Mo content (56.43%)
- Impurities (identity and concentrations): no relevant impurities > 1.0%
- Purity test date: 1990-06-06
- Storage condition of test material: at room temperature
No further significant information on test material was stated.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CD
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: approximately four to six weeks of age in the main study
- Weight at study initiation: weight range of 110 to 135 g in the main study
- Fasting period before study: overnight prior to dosing
- Housing: housed in groups of up to five rats of the same sex in metal cages with wire mesh floors
- Diet (ad libitum): standard laboratory rodent diet (SDS LAD 1)
- Water (ad libitum): domestic quality potable water
- Acclimation period: eight days prior to the start of the main study
ENVIRONMENTAL CONDITIONS
- Temperature: mean daily minimum and maximum temperatures of the animal room were 24°C and 29°C respectively
- Humidity: mean daily relative humiditiy value was 67 % R.H.
- Air changes: approximately 15 air changes/hour
- Photoperiod (hrs dark / hrs light): 12 hour/12 hour
No further significant details on test animals were stated.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- Preliminary study:
A trial test was carried out to establish a dosing regimen for the main study.
Ammonium molybdate was prepared at various (w/v) concentrations in corn oil and administered at a volume of 10.0 ml/kg bodyweight. the test sunstance was prepared on the day of dosing.
Concentrations in vehicle: 32 % w/v, 40% w/v, 50% w/v (main study)
Main study:
The initial dose level was selected on the basis of the preliminary study. Further groups were dosed, after review of the results, to obtain a dose response curve and permit estimation of a median lethal dose.
Treatment procedure:
The appropriate dose volume of the test substance was administered to each rat using a syringe and plastic catheter (8 choke). The day of dosing wa designated Day 1.
No further significant details on oral exposure were stated. - Doses:
- Preliminary study: 250 mg/kg bodyweight
Main study: 3200, 4000, 5000 mg/kg bodyweight - No. of animals per sex per dose:
- Preliminary study: 2 males/2 females
Main study: 5 males/5 females - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: Preliminary study: 5 days; main study: 14 days; Test animals fasted approximately 4 hours after dosing.
- Frequency of observations and weighing: Preliminary study and main study: Animals were observed soon after dosing and at frequent intervals for the remainder of Day 1 (a minimum period of five hours). On subsequent days the animals surviving treatment were observed once in the morning and again at the end of the experimental day. Clinical signs were recorded at each observation. Main study: The individual bodyweights of rats were recorded on Days 1 (day of dosing), 8 and 15 or at death.
- Necropsy of survivors performed: yes
- Other examinations performed: Main study: The nature, severity, approximate time of onset and duration of each toxic sign were recorded. Also the approximate time of death of individidual rats was recorded.
All surviving animals on the main study were killed on Day 15 by carbon dioxide asphyxiation. All animals that died during the study and those killed on Day 15 were subjected to a macroscopic post mortem examination which consisted of opening the cranial, abdominal anfd thoracic cavities. The macroscopic appearance of all examined tissues was recorded, and all livers and kidneys were preserved in buffered 10% formalin in order to satisfy any possible future requirement for further examination of these tissues.
No further information on study design were stated. - Statistics:
- The data obtained from this study did not permit the fitting of a probit line using the standard method (Finney 1971, Probit Analysis, 3rd Edition, Cambridgeshire University Press). However, it was possible to fit a line if a fixed slope was assumed; a value of 8.333 (estimated from background data which consited of the average slope for all LD50 estimations carried out in the Department of Industrial Toxicology over a period of one year) was used. Confidence intervals using this approach should be interpreted as minimum intervals since uncertainty in estimating the slope is not allowed for.
A test for a difference between the sexes was carried out on the individual responses using a test for trend in contingency tables with dose as a stratifying factor (Mantel, N. (1963) J. Amer. Statist. Ass. 58: 690-700).
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 883 mg/kg bw
- 95% CL:
- >= 3 371 - <= 4 468
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 3 884 mg/kg bw
- 95% CL:
- >= 3 178 - <= 4 735
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 3 883 mg/kg bw
- 95% CL:
- >= 3 178 - <= 4 735
- Mortality:
- There were deaths following a single oral dose of Ammonium molybdate among male and female rats dosed at 4000 mg/kg bodyweight (females: 3/5;males: 3/5) and 5000 mg/kg bodyweight (females: 5/5; males: 5/5). Deaths occured from within one hour of dosing to within 5 hours of dosing.
- Clinical signs:
- Pilo-erection was observed in all rats within five minutes of dosing and throughout the remainder of Day 1. This sign was accompanied on Day 1 and/or at later intervals by:
-abnormal body carriage (hunched posture) and abnormal gait (waddling) in 5/5 males and 5/5 femlaes dosed at 3200 and 4000 mg/kg, and in 3/5 male rats treated at 5000 mg/kg;
- lethargy in 5/5 males and 5/5 female rats dosed at 3200 mg/kg, in 3/5 males and 2/5 females dosed at 4000 mg/kg, and in 3/5 males and 3/5 females dosed at 5000 mg/kg
- decreased respiratory rate in 5/5 males and 5/5 females dosed at 3200 mg/kg, in 3/5 males and 2/5 females dosed at 4000 mg/kg and 3/5 males, and 3/5 females dosed at 5000 mg/kg
- pallor of the extremities in 5/5 males and 5/5 females dosed at 3200 mg/kg and 4000 mg/kg, and in 3/5 males and 3/5 females dosed at 5000 mg/kg
- ptosis in 3/5 males and 2/5 females dosed at 4000 mg/kg, and in 3/5 males and 3/5 females dosed at 5000 mg/kg
- diarrhoea in 5/5 males and 4/5 females dosed at 4000 mg/kg, and in 3/5 males and 3/5 females dosed at 5000 mg/kg
- ataxia in 1/5 males and 3/5 females dosed at 5000 mg/kg
Recovery of surviving rats, as judged by external appearance and behaviour, was complete by Day 3 (4000 mg/kg) or Day 5 (3200 mg/kg). - Body weight:
- Bodyweight losses or no change in bodyweight were recorded for all decedents.
Slightly low bodyweight gains were recorded for 5/5 surviving males at 3200 and 4000 mg/kg, and 1/5 female rat dosed at 4000 mg/kg on Day 8, and for 3/5 females treated at 3200 mg/kg on day 15. These rats achieved anticipated bodyweight gains on Days 15 and 8 respectively; all other rats achieved anticipated bodyweight gains throughout the study. - Gross pathology:
- Autopsy of rats that died during the study revealed thickening of the glandular region of the stomach in all rats dosed at 5000 mg/kg as the only macroscopic abnormality.
Terminal autopsy revealed no macroscopic abnormalities.
Any other information on results incl. tables
Preliminary study:
The results of the preliminary study indicated that the acute median lethal oral dose to male and female rats of Ammonium molybdate was greater than 250 mg/kg bodyweight. As a result of the limited toxicity seen at this dosage (pilo-erection was recorded for all rats on Day 1 only), an initial treatment level of 5000 mg/kg bodyweight was decided upon for the main study.
Estimation of LD50 values:
Combined sexes:
When a fixed slope of 8.333 was assumed the acute median lethal oral dose of Ammonium molybdate and its 95% confidence limits were estimated to be 3883 (3371 to 4468) mg/kg bodyweight.
Separate sexes:
When a fixed slope of 8.333 was assumed to the values were:
males: 3884 (3178 to 4735) mg/kg bodyweight
females: 3883 (3178 to 4735) mg/kg bodyweight
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
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