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EC number: 219-376-4 | CAS number: 2426-08-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
- Principles of method if other than guideline:
- To evaluate the in vitro genotoxicity of the test material an Ames test was performed on the Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, TA1538 with and without a metabolic activation system (S-9).
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Butyl 2,3-epoxypropyl ether
- EC Number:
- 219-376-4
- EC Name:
- Butyl 2,3-epoxypropyl ether
- Cas Number:
- 2426-08-6
- Molecular formula:
- C7H14O2
- IUPAC Name:
- 2-(butoxymethyl)oxirane
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Test material is indicated as R0065 in the study report.
Method
- Target gene:
- his revertants
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Remarks:
- and TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver Homogenate (S-9)
- Test concentrations with justification for top dose:
- 8.2, 24.7, 74.0, 222.2, 666.7, and 2000 µg/plate.
Based on preliminary toxicity testing. - Vehicle / solvent:
- Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- other: Methylnitronitrosoguanidine (MNNG) for strain TA1535 and TA100 without metabolic activation and, 2-aminoanthracene for all strains with metabolic activation.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation);
NUMBER OF REPLICATIONS: 3
To each of 2 mL of complete top agar, 0.1 mL of an overnight broth culture of each tester strain, 0.1 ml of the test material or diluent and 0.5 mL of the S-9 mix for the activated test will be added. The contents of the tube will be mixed thoroughly and poured onto VBE minimal agar plates. Plates will be gently rotated and tilted to assure uniform distribution of the top agar, allowed to harden on an even surface for 1 hour, inverted and put in a dark 37±0.5 °C incubator. After two days, the colonies in both test plates and controls were counted. - Evaluation criteria:
- The following criteria were established in the testing laboratory:
- Demonstration of toxicity of the chemical for the S. typhimurium strains
- The solvents control are within normal range, and
- Confirmation of sensitivity and responsiveness of the tester strains to mutagenic action.
If above mentioned criteria are met, a chemical that exhibits a positive, dose-related response over 3 concentrations with the baseline increase equal to twice the solvent control is considered to be mutagenic.
A chemical will be considered negative if the maximum non-inhibitory level exhibits less than a twofold increase in the number of induced revertants when compared to the solvent control. A non-linear dose response over three concentrations of the test substance will indicate mutagenic potential, but a dose-related response provided more persuasive evidence of mutagenesis.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Remarks:
- and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium, other: TA 1537, TA 1538, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The sensitivity of TA1535 to the action of the test material was demonstrated over 6 concentrations and activation improved this response at the 666.7 and 2000 µg/plate levels. In contrast, increased revertant numbers of TA100 were recorded only at the 3 highest concentrations of the test material and no substantial enhancement was obtained with the addition of a metabolic activation system (S-9).
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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