Castor oil, ester with glycerol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 Mar - 01 Apr 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (no purity, no data on pH and dissolved oxygen)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 1992

Deviations:

yes

Remarks:

No data on pH and dissolved oxygen is reported

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion in 20 L of tap water for each test concentration.

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Neil Hardy Aquatica, Surrey, UK
- Length at study initiation (length definition, mean, range and SD): 5.6 ± 0.2 cm
- Weight at study initiation (mean and range, SD): 1.94 ± 0.17 g

ACCLIMATION
- Health during acclimation (any mortality observed): < 1% in the 7 days prior to start of test, no medication

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

approx. 100 mg/L as CaCO3

Test temperature:

21 °C

pH:

no data

Dissolved oxygen:

no data

Nominal and measured concentrations:

Nominal: 0, 100, 180, 320, 560 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 L
- Aeration: via narrow bore glass tubes
- Renewal rate of test solution (frequency/flow rate): Daily renewal of test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 0.97 bodyweight/L; corpulence factor K = 1.1 g/cm3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (by the addition of Sodium thiosulphate)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortalities and behaviour were recorded at 3, 6, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8
- Range finding study: yes, but no details given.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

130 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL = 100 - 180 mg/L

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: no mortalities
- The results of the test solution analysis showed an increasing carbon concentration with increasing nominal test concentration and that no significant change in carbon concentration was observed over the 96 h exposure period; the results were taken to demonstrate the stability of the test concentrations employed during the period of exposure.

Sublethal observations / clinical signs:

Table 1: Additional information on effect concentrations

Time	Endpoint	Effect conc.	Nom./Meas.	Conc. based on	Basis for effect	Remarks
3 h	LC50	> 1000 mg/L	nominal	test mat.	mortality	-
6 h	LC50	800 mg/L	nominal	test mat.	mortality	95% CL = 710 – 900 mg/L
24 h	LC50	300 mg/L	nominal	test mat.	mortality	95% CL = 250 – 360 mg/L
48 h	LC50	130 mg/L	nominal	test mat.	mortality	95% CL = 100 – 180 mg/L
72 h	LC50	130 mg/L	nominal	test mat.	mortality	95% CL = 100 – 180 mg/L
96 h	LC50	130 mg/L	nominal	test mat.	mortality	95% CL = 100 – 180 mg/L

 

Highest test concentration resulting in 0% mortality = 100 mg/L

Lowest test concentration resulting in 100% mortality = 180 mg/L

Table 2: Cumulative Mortality Data for Golden Orfe Exposed for 96 h to the test substance

Nominal Concentration of test substance (mg/L)	Cumulative Mortality (initial population: 10)
	3 h	6 h	24 h	48 h	72 h	96 h
Control	0	0	0	0	0	0
100	0	0	0	0	0	0
180	0	0	0	10	10	10
320	0	0	6	10	10	10
560	0	0	10	10	10	10
1000	0	8	10	10	10	10

Table 3: Marked reactions other than death

Abnormality	Test Concentration	Time	Number of Fish
Loss of equilibrium	1000 mg/L	3 h	10/10
	560 mg/L	6 h	7/10
Presence of moribund fish	1000 mg/L	6 h	2/2
	560 mg/L	6 h	3/10
Lethargy	320 mg/L	24 h	4/4

Table 4: Results from Total Organic Carbon (TOC) Analysis

Samples	Nominal Concentration (mg/L)	Concentration of TOC corrected for control (mg C/L)
0 h (fresh media)	100	60.07
	180	97.19
	320	187.19
	560	331.79
	1000	572.89
24 h (old media)	100	51.258
	180	84.198
	320	162.138
	560	259.538
	1000	497.538
72 h (fresh media)	100	52.031
96 h (old media)	100	50.147

Description of key information

The study was conducted according to OECD 203.

LC50 (96h) = 130 mg/L

NOEC (96h) = 100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

130 mg/L

Additional information

The study with Castor oil, ester with glycerol (CAS 68459-67-6) was performed according to OECD 203 (GLP compliance not specified) under semi-static conditions using the freshwater species Leuciscus idus as test organism. The fish were exposed to nominal concentrations of 0, 100, 180, 320, 560 and 1000 mg/L over a period of 96h.. The highest test concentration resulting in 0% mortality was 100 mg/L. The lowest test concentration resulting in 100% mortality was 180 mg/L. The LC50 (96h) was determined to be 130 mg/L.