Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, aluminium salt

Endpoint summary

Description of key information

Short term toxicity to fish

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt. The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L andZebra Fish (Danio rerio)were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L.I t can be concluded from the value that the Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt is not toxic to the  aquatic evironment .

Short term toxicity to aquatic invertebrates

The Determination of the inhibition of the mobility of daphnids was carried out with the substance Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl) azo]-4-hydroxy naphthalene-1-sulphonateaccording to OECD Guideline 202.A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours.The median effective concentration (EC50) for the test substance, Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate, in Daphnia magna was determined to be >100 mg/L for immobilisation effects. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance , Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna).

Toxicity to aquatic algae and cyanobacteria

The effect of test item Hydrogen [4-[4-(diethylamino)-2',4'- disulphonatobenzhydrylidene] cyclohexa- 2,5-dien-1-ylidene] diethylammonium, sodium salt, (CAS No.129-17-9) was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l, 100 mg/l and 200 mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 123.386 mg/L. Thus, based on this value, it can be concluded that the test chemical can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Additional information

Short term toxicity to fish

Following experimental study from ABITEC for structurally similar read across Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] die...; sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)cyclohexa-2,5-dien-1-ylidene]methyl}benzene-1,3-... (CAS: 129-17-9) suggests that theFish Acute Toxicity test according to OECD Guideline 203 was conducted for Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt. The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L andZebra Fish (Danio rerio)were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L.I t can be concluded from the value that the Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt is not toxic to the  aquatic evironment amd can be considered as "not classified" as per the classification criteria.

It can be concluded from the above expiemental study that the Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, aluminium salt (CAS:15876-40-1) not toxic to the aquatic environment.

Short term toxicity to aquatic invertebrates

Following studies include the experimental studies for the structurally similar read across to conclude the toxicity extent of Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, aluminium salt (CAS: 15876-40-1) towards aquatic invertebrate is summarized as follows:

Experimental study from ABITEC, s. r. o forstructurally similar read across

2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-[(4-sulfo-1-naphthalenyl), sodium salt; Amaranth dye; trisodium 3-hydroxy-4-[(4-sulfonato-1-naphthyl)diazenyl]naphthalene-2,7-disulfonate (CAS: 915 -67 -3) suggests that theDetermination of the inhibition of the mobility of daphnids was carried out with the substance Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl) azo]-4-hydroxy naphthalene-1-sulphonateaccording to OECD Guideline 202.

A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours.The median effective concentration (EC50) for the test substance, Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate, in Daphnia magna was determined to be >100 mg/L for immobilisation effects. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance , Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna).

Similar experimental study forstructurally similar read across

disodium 3-[(2,4-dimethyl-5-sulfonatophenyl)diazenyl]-4-hydroxynaphthalene-1-sulfonate (CAS: 4548-53-2) also suggests that the

Determination of the inhibition of the mobility of daphnids was carried out with the substance Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl) azo]-4-hydroxy naphthalene-1-sulphonateaccording to OECD Guideline 202.A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours.The median effective concentration (EC50) for the test substance, Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate, in Daphnia magna was determined to be >100 mg/L for immobilisation effects. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance , Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna).

Thus based on the effect concentrations which is 100 mg/l gives the conclusion that test substance Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, aluminium salt (CAS: 15876-40-1) was likely to be not toxic to invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Following studies include the experimental studies for the structurally similar read across to conclude the toxicity extent of Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, aluminium salt (CAS: 15876-40-1) towards aquatic algae is summarized as follows:

Expermental study from ABITEC for test item Hydrogen [4-[4-(diethylamino)-2',4'- disulphonatobenzhydrylidene] cyclohexa- 2,5-dien-1-ylidene] diethylammonium, sodium salt, (CAS No.129-17-9) was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l, 100 mg/l and 200 mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 123.386 mg/L. Thus, based on this value, it can be concluded that the test chemical can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Similar Experimental study from ABITEC, s. r. o forstructurally similar read across2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-[(4-sulfo-1-naphthalenyl), sodium salt; Amaranth dye; trisodium 3-hydroxy-4-[(4-sulfonato-1-naphthyl)diazenyl]naphthalene-2,7-disulfonate (CAS: 915 -67 -3) suggests that

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 2,7-Naphthalenedi- sulfonic acid, 3-hydroxy-4-[(4-sulfo- 1-naphthal - enyl), sodium salt (Amaranth dye) according to OECD Guideline 201. The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 12.5, 25, 50, 100, 200 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, 2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-[(4-sulfo-1- -naphthalenyl), sodium salt, in Desmodesmus subspicatus was determined to be 356.2 mg/L. This value indicates that the substance is likely to be non-hazardous to aquatic algae and can not be classified as toxic as per the CLP criteria.

Further is also assissted by the Experimental study from ABITEC, s. r. o forstructurally similar read across

disodium 3-[(2,4-dimethyl-5-sulfonatophenyl)diazenyl]-4-hydroxynaphthalene-1-sulfonate (CAS: 4548-53-2)Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl) azo]-4-hydroxynaphthalene-1-sulphonate according to OECD Guideline 201.The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 0, 12.5, 25, 50, 100 and 200 mg/L. Effects on the growth rate of the organism were studied.The median effective concentration (ErC50) for the test substance, Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate, in Desmodesmus subspicatus was determined to be 276.1 mg/L. Based on this E_rC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus).

Thus based on the effect concentrations which is in range of 123.386 mg/l to 356.2 mg/L gives the conclusion that test substance Hydrogen [4-[4-(diethylamino)-2',4' disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, aluminium salt (CAS: 15876-40-1) was likely to be not toxic to algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.