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EC number: 286-924-7 | CAS number: 85392-65-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples taken from control media and each test concentration
- Sampling method: Samples were taken at test start and in fresh solutions at renewal at 48-hours, and in aged test solutions after 48 hours and at test end
- Sample storage conditions before analysis: Samples were acidified for stabilisation by addition of 2.5 mL of conc. nitric acid - Vehicle:
- no
- Details on test solutions:
- PREPARATION OF TEST MEDIUM
- Method: The quantity of test item required to obtain the desired concentrations was applied as an aqueous stock solution. The stock solution (containing 200 mg tetraammonium decachloro-mu-oxodiruthenate/L) was prepared in dilution water and stirred for approximately 2.5 hours. The individual test solutions were prepared by dilution of the respective stock solution with dilution water and sampled thereafter for chemical analysis. The test solutions were freshly prepared before test start and renewal after 48-hours.
- Controls: Untreated dilution water - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Danio rerio (Teleostei, Cyprinidae)
- Source: Test facility bred (West Aquarium GmbH, 37431 Bad Lauterberg, Germany)
- Length at study initiation: 2.0 ± 1 cm
- Holding water: Purified drinking water
- Holding conditions: Fish were fed ad libitum throughout the holding period with live brine shrimp (Artemia salina) nauplii, and ground flake food TetraMin® (Tetra Werke, Melle, Germany) once daily.
- Health: Only fish without diseases and abnormalities were used in the study (mortality < 5%).
- Feeding during test: None from 24 hours before test start
ACCLIMATION
- Period: Test fish were acclimatised for at least 12 days.
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Fed ad libitum, type not specified
- Feeding frequency: ad libitum
- Health during acclimation (any mortality observed): Less than 5% during the last week before the study - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- The total hardness of dilution water was 1.1 mmol/L
- Test temperature:
- The temperature was in the range of 22.9°C and 23.0°C.
- pH:
- The pH in the test vessels was between 7.38 and 8.66.
- Dissolved oxygen:
- The oxygen saturation in all test vessels was vetween 91% and 103%.
- Nominal and measured concentrations:
- The test concentrations applied were based on the results of a non GLP range finding test with nominal concentrations of 0.05, 0.5 and 5 mg tetraammonium decachloro-mu-oxodiruthenate/L. No effect was observed over the whole test period at any of the tested concentrations. For the definitive test, the highest test level was set at 100 mg/L, which is in line with the criteria of the OECD-guideline regarding the choice of test concentrations.
The concentration of ruthenium in the test solution was analysed at test start and at each renewal in fresh medium and at the respective end of the incubation period (48-hours each). In the freshly prepared test solutions, ruthenium concentrations between 82.6% and 100.0% of the nominal amount were measured. In aged test solutions, ruthenium concentrations were found to range between 51.6 and 113.9% of nominal concentration. Since the measured concentrations were outside a range of 80 to 120% of nominal throughout the test, geometric means of the concentrations measured in fresh and aged test solutions were calculated. As an initial step, the arithmetic mean values of both fresh and aged test solutions were calculated, considering the measured ruthenium concentrations at the test start and at 48 hours and at test end. Measured test item concentrations were calculated based on a ruthenium content of 30.26%.
Nominal concentrations: 5.68, 11.40, 22.70, 45.60 and 100.00 mg/L.
Geometric mean concentrations: 1.34, 3.11, 5.71, 11.32, 22.75 mg ruthenium/L
Corresponding to 4.44, 10.27, 18.87, 37.39 and 75.19 mg tetraammonium decachloro-mu-oxodiruthenate/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Aquarium
- Material, size, headspace, fill volume: Glass, 12 L with 10 L of test solution
- Aeration: slightly aerated via a glass capillary
- Renewal rate of test solution: test solution replaced after 48 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified drinking water (filtered through activated charcoal, passed through a limestone column and aerated)
- Culture medium different from test medium: No
- Intervals of water quality measurement: daily for temperature, pH and dissolved oxygen; ruthenium concentrations at 0, 48 and 96 hours
OTHER TEST CONDITIONS
- Photoperiod: 12 hours light: 12 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, sub-lethal and behavioural signs recorded at 3, 24, 48, 72 and 96 hours - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 75.19 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Remarks:
- Tetraammonium decachloro-mu-oxodiruthenate
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 22.75 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Remarks:
- Ruthenium
- Basis for effect:
- mortality (fish)
- Details on results:
- Neither significant signs of disease nor stress (discolouration or abnormal behaviour) were observed up to a concentration of 10.27 mg Tetraammonium decachloro-mu-oxodiruthenate/L (geometric mean) during the test period. Due to the dark colour of the test media at concentrations 18.87 to 75.19 mg/L, it was not possible to adequately observe signs of disease or stress. Fish presented comparable activity to control fish at the water surface. No fish died during the course of the study.
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The study was designed as a semi-static test. Thus, where the test results show inhibition around 50%, they should normally be statistically analysed by Probit analysis, assuming log-normal distribution of the values in order to determine an LC50and LC10value together with 95% confidence intervals. However, no statistical analysis was performed as no mortality was detected throughout the study. Thus, the LC50 is reported as being > as the nominal (or measured) concentration tested.
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality did not exceed 10% in the controls. The dissolved oxygen concentration was ≥60% in test vessels throughout the test.
- Conclusions:
- The 96 hour LC50 is > 75.19 mg tetraammonium decachloro-mu-oxodiruthenate/L corresponding to >22.75 mg/L ruthenium.
- Executive summary:
Zebrafish (Danio rerio) were exposed to the test substance at a range of 5 test item concentrations for 96 hours under semi-static conditions. The test was performed according to OECD guideline 203 and EC method C.1. Mortality and sub-lethal effects (including loss of equilibrium and change in swimming behaviour) were determined after 3, 24, 48, 72 and 96 hours. Test solutions were renewed after 48 hours, and fresh and aged test solutions were analysed by ICP-OES. Based on analysis of ruthenium, test concentrations were outside 80 – 120% of nominal, and therefore geometric mean measured concentrations were used for the reporting of results.
Neither significant signs of disease nor stress were observed at test concentrations up to 10.27 mg tetraammonium decachloro-mu-oxodiruthenate/L.Due to the dark colour of test media at
concentrations 18.87 to 75.19 mg/L, it was not possible to adequately observe signs of disease or stress, but fish near the surface of the water were active. No fish died during the course of the study. The LC50 was determined to be > 75.19 mg tetraammonium decachloro-mu-oxodiruthenate/L, corresponding to > 22.75 mg Ru/L.
Reference
Description of key information
The 96 hour LC50 is > 75.19 mg tetraammonium decachloro-mu-oxodiruthenate L-1 corresponding to >22.75 mg Ru L-1.
Key value for chemical safety assessment
Additional information
Zebrafish (Danio rerio) were exposed to the test substance at a range of 5 test item concentrations for 96 hours under semi-static conditions (Teigeler 2016). The test was performed according to OECD guideline 203 and EC method C.1. Mortality and sub-lethal effects (including loss of equilibrium and change in swimming behaviour) were determined after 3, 24, 48, 72 and 96 hours. Test solutions were renewed after 48 hours, and fresh and aged test solutions were analysed by ICP-OES. Based on analysis of ruthenium, test concentrations were outside 80 – 120% of nominal, and therefore geometric mean measured concentrations were used for the reporting of results.
Neither significant signs of disease nor stress were observed at test concentrations up to 10.27 mg tetraammonium decachloro-mu-oxodiruthenate L-1.Due to the dark colour of test media at
concentrations 18.87 to 75.19 mg L-1, it was not possible to adequately observe signs of disease or stress, but fish near the surface of the water were active. No fish died during the course of the study. The LC50 was determined to be > 75.19 mg tetraammonium decachloro-mu-oxodiruthenate L-1, corresponding to > 22.75 mg Ru L-1.
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