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EC number: 274-950-1 | CAS number: 70865-20-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Apr - 11 May 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sodium 4-[[4-[(4-hydroxy-2-methylphenyl)azo]phenyl]amino]-3-nitrobenzenesulphonate
- EC Number:
- 274-950-1
- EC Name:
- Sodium 4-[[4-[(4-hydroxy-2-methylphenyl)azo]phenyl]amino]-3-nitrobenzenesulphonate
- Cas Number:
- 70865-20-2
- Molecular formula:
- C19H16N4O6S.Na
- IUPAC Name:
- sodium 4-[[4-[(4-hydroxy-2-methylphenyl)azo]phenyl]amino]-3-nitrobenzenesulphonate
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mL and control
- Sampling method: 10 mL taken from approximate centre of test vessel at beginning of test
- Sample storage conditions before analysis: -20°C
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.5 g test item directly added to test vessel (volume 15L) and suspended
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): turbid suspension
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: P Hohler, Zeinigen, Switzerland
- Length at study initiation (mean): 4.6 - 6.0 cm
- Weight at study initiation (mean): 1.5 g (control), 1.4 g (treatment)
ACCLIMATION
- Acclimation period: 7 d
- Acclimation conditions (same as test or not): tank 250 L in reconstituted water at 14.5 - 15.0°C with daily feeding
- Health during acclimation (any mortality observed): 0%
FEEDING DURING TEST
No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 250 mg CaCO3 /L (dilution water)
- Test temperature:
- 14.0 - 14.5°C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- 8.9 - 11.6 mg/L
- Nominal and measured concentrations:
- 100 mg/L (nominal)
70.5 mg/L (measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel: tank
- Material, size, headspace, fill volume: 15 L
- Aeration: yes
- No. of organisms per vessel: 10 (treatment), 7 (control)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h (light) / 8h (dark)
- Light intensity: 500 - 1500 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, clinical signs, size, body weight
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test
- Range finding study
- Test concentrations: 0, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: yes, limit test in main study due to 0% mortality and no clinical signs in range finding study - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 70.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 70.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no effect
- Other biological observations: no
- Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: turbid suspension and particles adsorbing to the vessel might have caused a mean recovery of ca. 70% after 96 h.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96-h LC50 to fish is >70.5 mg/L (mean measured). No clinical signs of intoxication (sublethal effects) were observed during the test.
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