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EC number: 246-874-9 | CAS number: 25340-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This is a GLP guideline study.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-4 (Acute Eye Irritation)
- Deviations:
- no
- Remarks:
- Not specified in report.
- Principles of method if other than guideline:
- Npt applicable
- GLP compliance:
- yes
Test material
- Reference substance name:
- Diethylbenzene
- EC Number:
- 246-874-9
- EC Name:
- Diethylbenzene
- Cas Number:
- 25340-17-4
- Molecular formula:
- C10H14
- IUPAC Name:
- diethylbenzene
- Details on test material:
- IUCLID4 Test substance: as prescribed by 1.1 - 1.4
MCS 2313; Diethylbenzene
Supplier: Monsanto
Reference No.: Lot/batch No. MBP 3494320
Appearance: Clear, colorless liquid
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Source: New Zealand white rabbit; Summit View Farms, Hazleton, Pennsylvania
At start of the experiment, animals were at least 8 weeks old.
Equilibration period: 20 days
Observations : All animals were checked for viability twice daily. Prior to assignment to study all animals were examined to ascertain suitability for study.
Husbandry: Currently acceptable practices of good animal husbandry were followed, e.g. Guide for the Care and Use of Latoratory Animals: DHEW Publication No. (NIH) 78-23 Revised 1978
Housing: Individually housed during study.
Cages: Suspended, stainless steel
Environmental Conditions: Temperature: 60-70F is considered an acceptable tmperature range for rabbits; room temperature was monitored and recorded twice daily and maintained within this range to the maximum extent possible.
Humidity: 30-70% is considered an acceptable humidity range for rabbits; room humidity was monitored and recorded daily and maintained within this range to the maximum extent possible.
Light Cycle: 12 hours light, 12 hours dark (controlled by a n automatic timer).
Food:Lab Rabbit Chow HF, (Purina #5326), ad libittum
Water: Automatic watering system, ad libitum. Municipal water supply (Elizabethtown Water Co.)
Contaminants: There were no known contaminants reasonably expected to be found in food or water which would interfere with the results of this study.
Identification: Each animal was identified with a monel eat tag, bearing a unique number, prior to testing.
Selection: Animals were randomly placed in cages upon receipt, and were placed on study as available at the time of study initiation. Any animals considered unsuitable because of poor health or ocular abnormalities were excluded from selection.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- 0.1 ml
- Duration of treatment / exposure:
- single dose
- Observation period (in vivo):
- 3-7 days
- Number of animals or in vitro replicates:
- 6 (3/sex)
- Details on study design:
- Duration of Study:
A single dose w as admlnlstered followed by a 3 to 7 day observation period.
Route of Administration : Ocular
Justification for Route of Adnlnlstratlon: The study was intended to provide information on the health hazards likely to arise from a short-term accidental exposure to the test material by the ocular route.
Preparation of Animals:
On the dry before doslng, both eyes of each animal were examined usilng fluorescein dye to check for presence of cornerl ulceration. Just
prlor to test substance application, the eyes were examined again, but without fluorescein. Animals showing pre-existing corneal or
conjunctival injury or irritation were not placed on study.
Preparation of Test Material: The test material was administered as received; no mixture was required.
Administration of Test Substance:
The appropriate amount of the test material was introduced into the lower conjunctivall sac of the right eye of each animal. The upper and lower lids were gently held together for one second prlor to releasing to prevent loss of material. The contralateral eye served as the control.
Wash:
No wash was performed after application of the material. However, approximately 24 hrs after application, the treated eyes were rinsed to remove any residual test material.
Viability Checks:
Twice daily
Evaluation of Ocular Irritation:
1. Intervals
Approximately 1, 24, 48 and 72 hours and 7 days after treatment or until no signs of irritation were present.
2. Methods
At each interval the treated and control eyes were examined and scored for ocular reactions according to the Draize scale. Fluorescein dye was used to confirm presence or absence of corneal ulceration in treated eyes starting with the 24 hour observation and at each subsequent observation until there was no stain retention for two observations.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Remarks:
- Redness
- Basis:
- mean
- Remarks:
- over 6 rabbits
- Time point:
- 24/48/72 h
- Score:
- 1.3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Remarks:
- over 6 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0.2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- conjunctivae score
- Remarks:
- Discharge
- Basis:
- mean
- Remarks:
- over 6 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- over 6 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- over 6 aniumals
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- Produced mild but reversible ocular irritation. Irritation consisted primarily of conjuctival irritation (redness, chemosis, discharge) and iridial changes. No corneal changes were seen. All six animals were free of ocular irritation within 3 to 7 days after instillation of the test material
- Other effects:
- None
Any other information on results incl. tables
Tissue | Endpoint | 24, 48, 72 h scores mean | MEAN SCORE | |||||
Animal No | ||||||||
2781 | 2782 | 2783 | 2788 | 2791 | 2792 | |||
Conjuctiva | Redness | 1.3 | 1 | 1.3 | 1.7 | 1.7 | 1 | 1.3 |
Chemosis | 0.3 | 0 | 0.3 | 0 | 0.3 | 0 | 0.2 | |
discharge | 0.6 | 1 | 0.6 | 1 | 0.6 | 0.3 | 0.7 | |
necrosis | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Iris | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Cornea | Opacity | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
area | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
stippling | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
ulceration | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information not classifiable Criteria used for interpretation of results: EU
- Conclusions:
- Produced mild but reversible ocular irritation. Irritation consisted primarily of conjuctival irritation (redness, chemosis, discharge) and iridial changes. No corneal changes were seen. All six animals were free of ocular irritation within 3 to 7 days after instillation of the test material. BAsed on the results of this study, no classification for eye irritation would be necessary.
- Executive summary:
This study was conducted for the Monsanto Company to evaluate the ocular irritation produced by MCS 2313 in rabbits. The eyes of six rabbits were instilled with undiluted test material and left unwashed. Ocular observations for irritancy were evaluated at ~1, 24, 48 and 72 hours and 7 days after treatment or until no signs of irritation wre present.
MCS2313 produced mild but reversible ocular irritation. Irritation consisted primarily of conjuctival irritation (redness, chemosis, discharge) and iridial changes.
No corneal changes were seen. All six animals were free of ocular irritation within 3 to 7 days after instillation of the test material.
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