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EC number: 205-429-9 | CAS number: 140-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 04 to November 10, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100, 45.5, 20.7, 9.39 and 4.27 mg/l loading rate
- Sampling method: the test solutions were prepared by respective dilutions of a saturated solution. The respective quantities of test item were added to an empty glass vessel. - Vehicle:
- yes
- Remarks:
- Water
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Straus, 1820)
- Breeding: Parental and young Daphnia held in 4 l glass aquaria (3 l medium) at 20 ± 2 °C, fed with a suspension of Desmodesmus subspicatus in Elendt M4 medium with an optical density OD680 of about 15 units
- Age: less than 24 h
- Feeding during test: no - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 18 – 22 °C
- pH:
- 7.0 ± 0.5
- Conductivity:
- <1.5 µS/cm
- Nominal and measured concentrations:
- 100, 45.5, 20.7, 9.39 and 4.27 mg/l nominal loading rate
26.9, 11.9, 5.38, 2.38 and 1.06 mg/l measured concentration (27, 26, 26, 25 and 25 %, respectively, of the nominal loading rate) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml beakers
- Material, size, headspace, fill volume: glass, 100 ml, with 50 ml of test medium
- Aeretion: no, during the test the beakers were covered with a glass plate to avoid evaporation and contamination of the test solutions with dust
- Renewal rate of test solution (frequency/flow rate): renewal of the test medium every 24 h
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 3
OTHER TEST CONDITIONS
- Adjustment of pH: pH value adjusted to pH 7.0 ± 0.5
- Photoperiod:16 h photoperiod a day, supplied by overhead white fluorescent tubes
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: at 1 mg/l 0 % of immobilization after 48 h, at 10 mg/l 17.5 % of immobilization, at 100 mg/l 70 % of immobilization - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2.28 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 0.854–5.93 mg/l
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 7.76 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 6.08–9.13 mg/l
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.24 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 0.816 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 0.058–1.70 mg/l
- Duration:
- 24 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 3.61 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 1.35–5.01 mg/l
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48h) = 2.28 mg/l
- Executive summary:
Method
The median effect concentration (EC50) and the no-effect concentration (NOEC) of Intercure 3 to Daphnia magna were investigated under semi-static exposure conditions over a period of 48 h, following OECD guideline 202.
Since the test item is readily biodegradable, the test was performed under semi-static exposure conditions with renewal of the test medium after 24 h of exposure. The test media were prepared by dilutions of a saturated stock solution.
The nominal loading rates corresponding to the different dilutions were100, 45.5, 20.7, 9.39 and 4.27 mg/l.
40 individual Daphnia divided into 2 test vessels were exposed to each concentration of the test item, and 60 Daphnia divided into 3 test vessels were used as blank controls.
The concentrations of Intercure 3 in the test medium were measured by HPLC analysis at the beginning and after 24 and 48 h of exposure. These analyses confirmed that the test item was partly dissolved, that the concentrations were not stable over the whole 48 h period and that they did not remain within 80-120 % of the nominal loading rates. Therefore, the effective concentrations ECxwere assessed based on measured concentrations.
Results
The 48 h EC50 value of Intercure 3 of Daphnia magna was 2.28 mg/l. This value is based on measured concentrations.
All validity criteria were fulfilled.
Reference
Test concentrations
The concentrations of Intercure 3 in
the test medium were measured by HPLC analysis (LOQ 0.063 mg/l) at the
beginning, after 24 h in the old and the fresh media, and after 48 h of
exposure.
These analyses confirmed that the test item was partly dissolved and the
concentrations were not stable over the whole 48 h period and were not
within 80-120 % of the nominal loading rates.
The measured concentrations of the test item at the beginning of the
test were 26.9, 11.9, 5.38, 2.38 and 1.06 mg/l (27, 26, 26, 25 and 25%,
respectively, of the nominal loading rate) and 24.4, 7.11, 0.745, < LOQ
and < LOQ (24, 16, 4 % respectively of the three highest nominal
loadings; not applicable for the three lowest loadings) after 24 h in
the old media.
After renewal of the test media at 24h, the concentrations were 17.0, 7.58, 3.25, 1.36 and 0.520 mg/l (17, 17, 16, 14 and 12%, respectively, of the loading rate), and 15.6, 2.64, > LOQ > LOQ and < LOQ (16 and 6% of the two highest nominal loadings; not determined for the three lowest) at the end of the test.
Therefore, the effective concentrations ECx after 24h of exposure were assessed based on thegeometric mean of the measured concentrations at 0 and 24h; while the effective concentrations ECx after 48h of exposure were assessed based onthe arithmetic mean of the two 24-h periods’geometric means of the measured concentrations.
Details on results
- After 24 h, following immobilization rates were observed: 100 % at 100 mg/l and 65 % at 45.5 mg/l. No significant effects (≤ 10 % immobilization) were observed at the loading rates of 20.7, 9.39 and 4.27 mg/l or in the blank controls;
- After 48 h, following immobilization rates were observed: 100 % at 100 mg/l, 100 % at 45.5 mg/l and 12.5 % at 20.7 mg/l. No significant effects (≤ 10% immobilization) were observed at the loading rates of 9.39 and 4.27 mg/l or in the blank controls.
Description of key information
EC50 (48h) = 2.28 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 48 mg/L
Additional information
The median effect concentration (EC50) and the no-effect concentration (NOEC) of Intercure 3 to Daphnia magna were investigated under semi-static exposure conditions over a period of 48 h, following OECD guideline 202.
Since the test item is readily biodegradable, the test was performed under semi-static exposure conditions with renewal of the test medium after 24 h of exposure. The test media were prepared by dilutions of a saturated stock solution.
The nominal loading rates corresponding to the different dilutions were 100, 45.5, 20.7, 9.39 and 4.27 mg/l.
40 individual Daphnia divided into 2 test vessels were exposed to each concentration of the test item, and 60 Daphnia divided into 3 test vessels were used as blank controls.
The concentrations of Intercure 3 in the test medium were measured by HPLC analysis at the beginning and after 24 and 48 h of exposure. These analyses confirmed that the test item was partly dissolved, that the concentrations were not stable over the whole 48 h period and that they did not remain within 80-120 % of the nominal loading rates. Therefore, the effective concentrations ECxwere assessed based on measured concentrations. The 48 h EC50 value of Intercure 3 of Daphnia magna was 2.28 mg/l. This value is based on measured concentrations. All validity criteria were fulfilled.
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