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EC number: 206-376-4 | CAS number: 334-48-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Study period:
- 13 Oct - 17 Nov 1978
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Inconsistent results
- Qualifier:
- no guideline followed
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Sample was weighed in clean, dried and tared one milliliter beakers and then introduced into the CAD flask or BOD dilution bottle. - Test organisms (species):
- other: "biological seed"
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Remarks on exposure duration:
- no data
- Reference substance (positive control):
- no
- Details on results:
- No results are given in the study report. It is considered to be invalid, since the measured oxygen demand was higher than the theoretical oxygen demand.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- not reported
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Only short abstract available
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Bacillus subtilis was tested with different substances and the optical densitiy measured after 1 h to calculate the inhibition.
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Inoculum was placed into plastic tubes or Erlenmeyer flasks which already contained the test substance
- Controls: 2 with solvent, 2 with distilled water
- Chemical name of vehicle: Ethanol, dimethylsulfoxide, sodium hydroxide or distilled water - Test organisms (species):
- Bacillus subtilis
- Details on inoculum:
- - Pretreatment: Plated with tryptose blood agar base (33 g/L) were inoculated from single colonies of Bacillus subtilis and incubated for 7 h. Afterwards the cells were supended and diluted at different low titers into three flasks containing the MCV medium and shaken at room temperature overnight. If the cultures had not grown overnight beyond an optical density at 600 nm (OD600) of 1.0 it was not used. The cultures reaching 1.0 were utilised for the test.
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 60 min
- pH:
- 7.2, adjusted with KOH
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic tubes or Erlenmeyer flasks
- Aeration: Tubes were aerated.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MCV medium contains 1.4% K2HPO4 , 0.6% KH2PO4, 0.2% MgSO4 * 7 H2O, 0.1% sodium citrate, 25 µg/mL L-tryptophan, 10 µg/mL L-methionine and 1 % vitamin-free casein hydrolysate
OTHER TEST CONDITIONS
- Adjustment of pH: with KOH to 7.2
EFFECT PARAMETERS MEASURED: optical density at 600 nm after 60 min in the plastic tubes and after 15 or 20 min in the Erlenmeyer flasks - Reference substance (positive control):
- no
- Duration:
- 60 min
- Dose descriptor:
- EC50
- Effect conc.:
- 0.25 mmol/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- not reported
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- abstract
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Six different bacteria were anaerobically incubated and the turbitidy measured to determine inhibition on growth.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solution was prepared by dissolving measured quantities of the test substance in absolute ethanol. First a 250 mM stock solution was prepared and then further diluted with ethanol to 200, 175, 150, 125, 100, 75, 50, 25, 10, 5 and 2.5 mM.
- Controls: Since the test compund produces culture turbidity, a dilution series with medium and test compound without inoculum were prepared. - Test organisms (species):
- other: Bifidobacterium bifidum type a, Bifidobacterium bifidum type b, Bifidobacterium infantis sp infantis type a, Bifidobacterium infantis sp infantis type b, Escherichia coli, Salmonella typhimurium
- Details on inoculum:
- - Preparation of inoculum for exposure: The PYG medium was dispensed into a 500 mL screw-capped bottle and sterilised at 121 °C for 15 min. While cooling, 3.0 mL were delivered into metal capped culture tubes which were inoculated with 0.03 mL of the substance stock solutions (2.5 - 20 mM). The culture tubes were placed in an anaerobic cabinet and maintained at 37°C over night. Oxygen free gas (20% nitrogen in hydrogen) was supplied.
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 29 h
- Test temperature:
- 37 °C
- pH:
- 4.5 - 6.6, the pH was measured in the highest and lowest substance concentrations.
- Nominal and measured concentrations:
- Nominal: 200, 175, 150, 125, 100, 75, 50, 25, 10, 5 and 2.5 mM
- Details on test conditions:
- TEST SYSTEM
- Test vessel: culture tubes
- No. of vessels per control (replicates): one series (2.5 - 200 mM test substance) with test substance without inoculum to determine turbidity
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The PYG media (Petone-yeast extract glucose) with 1% glucose, was prepared and dispersed anaerobically and sterilised. Anaerobic culture counts were performed on PYG-agar roll tubes.
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: turbidity after 29 h - Reference substance (positive control):
- no
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: B. bifidum type a
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: B. bifidum type b
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: B. infantis sp infantis type a
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: B. infantis sp infantis type b
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: S. typhimurium
- Details on results:
- The test substance had a positive effect on E. coli growth.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 19 Feb 1988
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- study was performed for only 30 min
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 part 27
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- duration of exposure only 30 min.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: Strain Berlin 33/2 (DSM 50026)
- Preparation of inoculum for exposure: direct weight of test substance into test vessels, filled up to 100 mL with drinking water, 5 min treatment with ultrasound - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 100 mL Erlenmeyer flasks with 100 mL test medium
- Aeration: directly over the surface of the test solution
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water (copper- and chlorine-free)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption after 30 min
- Reference substance (positive control):
- no
- Duration:
- 30 min
- Dose descriptor:
- EC0
- Effect conc.:
- 3 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Referenceopen allclose all
Table 1: O2-consumption after 30 min
Concentration [mg/L] |
O2-consumption |
0 (control 1) |
2.7 |
0 (control 2) |
2.9 |
0 (control 3) |
3.2 |
0 (control 4) |
3.2 |
3000 |
2.4 |
10000 |
2.8 |
Description of key information
EC10 (18 h) = 912 mg/L (ISO 10712); RA CAS 124-07-2
Key value for chemical safety assessment
- EC50 for microorganisms:
- 912 mg/L
Additional information
Good quality read across data from octanoic acid are used as key information to assess the toxicity of decanoic acid to microorganisms, due to deficiencies of the available data on decanoic acid.
The toxicity of the read across substance octanoic acid towards Pseudomonas putida, is evaluated in a cell multiplication inhibition test (1988). The study was conducted similarly to ISO 10712. The test organism was exposed to a wide range of nominal concentrations (0.266 - 1088.00 mg/L) and an EC10 (18h) of 912 mg/L (nominal) was determined.
A further study, investigating the toxicity of the substance to Pseudomonas putida, is available for decanoic acid and was performed according to DIN 38412 part 27. The study resulted in an EC0 (30 min) of 3000 mg/L and an EC10 (30 min) of 10,000 mg/L based on respiration rate. However, the study was not used for further assessment due to the short duration of the experiment.
An additional study (1978) is available conducted with biological seed as the test organisms but was rated unreliable (RL3) due to inconsistent results and poor reporting.
Supportive information is withdrawn from short abstracts of two publications. In the first study (Freese, 1979) conducted with Bacillus subtilis, a EC50 of 0.25 mmol/L was obtained after 60 min. In the second study (Powell and May, 1981) with six different bacterial strains incubated anaerobically, different sensitivity of the various organisms to decanoic acid were observed. However, due to limited documentation and consequently limited information, these two literature studies were rated as not assignable.
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