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EC number: 210-695-4 | CAS number: 621-62-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ethane, 2-Chloro-1,1-Dimethoxy-
- IUPAC Name:
- Ethane, 2-Chloro-1,1-Dimethoxy-
- Reference substance name:
- 97-97-02
- IUPAC Name:
- 97-97-02
- Reference substance name:
- 2-chloro-1,1-dimethoxyethane
- EC Number:
- 202-624-0
- EC Name:
- 2-chloro-1,1-dimethoxyethane
- Cas Number:
- 97-97-2
- Molecular formula:
- C4H9ClO2
- IUPAC Name:
- 2-chloro-1,1-dimethoxyethane
- Test material form:
- other: liquid
- Details on test material:
- - Name: Chloroacetaldehyde dimethylacetal
- Physical state: colourless liquid
Constituent 1
Constituent 2
Constituent 3
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- without metabolic activation: 2.5, 5.0, 25.0, 50.0, 250.0, 500.0, 2500.0, 5000.0 µg/plate
with metabolic activation: 50.0, 250.0, 500.0, 2500.0, 5000.0 µg/plate
Controlsopen allclose all
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other:
- Remarks:
- Natrium Acid for TA100, TA1535 without metabolic activation
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- TA98 without metabolic activation
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: DMSO
- Remarks:
- TA1537 without metabolic activation
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: DMSO
- Remarks:
- TA98, TA100, TA1535, TA1537 with metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
Because of the test results the test substance Chloroacetaldehyde dimethylacetal shows mutagenic properties in strains TA98, TA100 and TA1535. - Executive summary:
Chloroacetaldehyde dimethylacetal was tested on its potential to dissolve gene mutation in the genomeof the Salmonella typhimurium TA98, TA100, TA1535 and TA1537 strains. Two independent tests were carried out independently with and without metabolic activation. The substance was tested in following concentrations:
Without metabolic activation: 2.5, 5.0, 25.0, 50.0, 250.0, 500.0, 2500.0, 5000.0 µg/plate
With metabolic activation: 50.0, 250.0, 500.0, 2500.0, 5000.0 µg/plate
Distilled water was chosen as solvent for the test substance because of its good solubility character. Precipitations caused of the test substance were noticed neither at the single dilutions nor in the agar. Every concentration including the controls was tested three times. Apart from TA98 all strains showed normal background growth in presence of the test solution at all concentrations. Strain TA98 showed lower background growth as well as a clearly reduced number of revertants at test concentrations 5000, 2500 and 500 µg/ plate in experiment I without metabolic activation. In experiment II the background growth was considerably declined as in controls at concentrations of 5000 and 2500 µg. These toxicological effects didn’t appear at metabolic activation.
Compared to the negative controls with concentrations of 5000 and 2500 µg/plate a significant and reproducible increase of the revertant numbers was noticed in test strain TA100. The revertant number of strain TA100 with metabolic activation was increased in experiment I (5000µg/plate; mutation factor 4.6), in experiment II this effect was observed as well at concentration 2500 µg/plate. At concentrations of 5000 µg and 2500 µg/plate mutation factors of 5.6 and 3.5 were determined here. In both experiments a mutagenic effect at test strain TA100 was proved through the test substance. The considerable mutation factor for the mutagenic effect of 2 was clearly exceeded. Reproducible dose dependent mutagenic effects were observed at concentrations of 5000 and 2500 µg/plate in experiment I (mutation factors 3.1 and 2.4) and experiment II (mutation factors 2.8 and 1.9) in presence of the liver microsomal fractionin test strain TA1535 and TA100 as well as a bacterium with a mutation regarding base pairing. At test strain TA1535 the increase of the revertant numbers was slightly lower without metabolic activation and a concentration of 5000 µg/plate, but it can be supposed of a mutagenic effect because the results were reproducible (mutation factors 1.9 and 1.8). Slightly increased revertant numbers were observed in both experiments in TA98 at the highest test concentration with metabolic activation (mutation factors 2.1 and 1.8). No mutagenic properties of the test substance in all concentrations were shown in TA1537. The recommended area of the spontaneous reversion rates (3.4) referred to the negative controls without metabolic activation was never left at the mean rate of 3 plates. Suitable reference substances showed clear increases of the revertant numbers at the test strains and confirmed the enzymatic activity of the liver microsomal fraction. Because of the test results the test substance Chloroacetaldehyde dimethylacetal shows mutagenic properties in strains TA98, TA100 and TA1535.
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