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Diss Factsheets
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EC number: 940-265-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005-01-14 to 2005-03-31
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP compliant; Guideline Study (OECD 201); Deficiencies: The coefficient of analysis of the daily growth rate in the control replicates and the coefficient of analysis of the average specific growth rates in the control replicates were not reported according to the current guideline adopted 2006.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- see Sect. "Rationale for Reliability incl. deficiencies"
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- see Sect. "Rationale for liability incl. deficiencies"
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentration: For the spiked samples of the test item 103.71 mg test item was dissolved in about 90 mL purified water, ultrasonicated for five minutes and made up to the mark in a 100 mL volumetric fiask with purified water to prepare a stock solution of 1037 mg test item/L.
- Sampling method: For the analytical measurements the following samples were taken: fourfold samples (without algae) from each test medium and the control just before the test start and after 72 h.
- Sample storage conditions before analysis: The samples were stored deep-frozen and protected from light until analysis was performed.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: WAFs (Water Accommodated Fractions) were prepared according to the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures as described below:
Prior to the start of the test, six test media with loading rates of 0.32, 1.0, 3.2, 10, 32 and 100 mg/L were prepared.
The test media with loading rates 100, 32, 10, and 3.2 mg/L were prepared by mixing 150, 48.1, 20.0, and 6.4 mg test item into 1500, 1500, 2000, and 2000 mL of test water respectively. Ultrasonic treatment was applied for 15 minutes. No auxiliary solvent or emulsifler was used. The dispersions were then stirred on a magnetic stirrer at room temperature in the dark for 96 hours to dissolve a maximum amount of the test item in test water. The long stirring period of 96 hours was chosen according to the results of a pre-test (without GLP) which showed that during this time no signiflcant loss of the test item was to be expected.
After the stirring period of 96 hours, the saturated dispersions were filtered through a membrane filter (Schleicher & Schuell, Type NC45, pore size 0.45 pm). The undiluted filtrates with dissolved test item were tested on algae as WAFs.
For practical reasons, the test media with loading rates 1.0 and 0.32 mg/L had to be prepared by diluting the WAF with the loading rate of 3.2 mg/L with test water. Additionally, a control was tested in parallel.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus subspicatus CHODAT,
- Strain: Strain No. 86.81 SAG
- Source (laboratory, culture collection): SAG, Institute for Plant Physiology, University of Göttingen, D-37073 Göttingen, Germany
- Age of inoculum (at test initiation): four days
- Method of cultivation: exponentially growing pre-culture
ACCLIMATION
- Acclimation period: one day
- Culturing media and conditions: same as test medium
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg/L as CaCO3
- Test temperature:
- 22 - 23 °C
- pH:
- 7.9 - 8.6
- Nominal and measured concentrations:
- Nominal WAF loading rates: 0.32, 1.0, 3.2, 10, 32, 100 mg/L
measured concentrations (main component):Since WAFs were tested, the reported biological results are based on the nominal loading rates. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL Erlenmeyer glass flasks
- Type: closed with glass dishes
- Material, size, headspace, fill volume: Volumes of 15 mL algal suspension for each replicate were continuously stirred by magnetic stirrers in 50 mL Erienmeyer flasks.
- Type of flow-through: No
- Renewal rate of test solution (frequency/flow rate): No
- Initial cells density: 10E04 cells per mL;
- Control end cells density: 69 x 10E04 cells/mL
- No. of vessels per concentration (replicates): three
- No. of vessels per control (replicates): six
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic test water, prepared according to the test guidelines. Analytical grade salts were dissolved in sterile purified water.
- Culture medium different from test medium: No
- Intervals of water quality measurement: At the beginning and at the end of the test period
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination;
- Light intensity and quality: 8500 to 9500 lux; - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 3.5 - 28
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 91 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 32 - 2039
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.5
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 0.28 - 12
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Unusual cell shape: no
- Colour differences: no
- Flocculation: no
- Adherence to test vessels: no
- Aggregation of algal cells: no - Reported statistics and error estimates:
- The EbC50 and ErC50 values (the respective concentrations of the test item corresponding to 50% inhibition of algal biomass (b) and growth rate (r) compared to the control), and the corresponding EC10 value and their 95%-confidence limits were calculated by Probit Analysis.
For the determination of the LOEC and NOEC, the calculated mean biomass and the mean growth rate at the test concentrations were tested for significant differences when compared to the control values by a Dunnett-test
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- With high probability not acutely harmful to aquatic algae. No toxic effects within the range of solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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