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Diss Factsheets
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EC number: 617-849-3 | CAS number: 86404-04-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Oct - 18 Dec 2015
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The study was performed without any technical deficiencies. However, the result of this study may underestimate the biodegradation of the test item since the substance turned out to be inhibitory to the microbial activity.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Health Care Inspectorate of the Ministry of Health, Welfare and Sport, Utrecht, The Netherlands
Test material
- Reference substance name:
- (5R)-5-[(1S)-1,2-dihydroxyethyl]-4-ethoxy-3-hydroxy-2,5-dihydrofuran-2-one
- EC Number:
- 617-849-3
- Cas Number:
- 86404-04-8
- Molecular formula:
- C8H12O6
- IUPAC Name:
- (5R)-5-[(1S)-1,2-dihydroxyethyl]-4-ethoxy-3-hydroxy-2,5-dihydrofuran-2-one
- Details on test material:
- - Name of test material (as cited in study report): Ethyl ascorbic acid
- Physical state: powder
- Expiration date of the lot/batch: 21 Apr 2018
- Stability under test conditions: stable
- Storage condition of test material: in freezer at < -15 °C desiccated
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Obtained from the municipal sewage treatment plant "Waterschap Aa en Maas", s'Hertogenbosch, The Netherlands
- Laboratory culture: no
- Storage conditions: The activated sludge was kept under continuous aeration until further treatment
- Pretreatment: Before use the sludge was allowed to settle (86 min) and the supernatant was used as inoculum at the amount of 10 mL/L of mineral medium. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 25.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12 mg/L
- Based on:
- other: TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Additional substrate: no
- Test temperature: 21.8 - 22.5 °C
- pH: 7.5 - 7.8
- pH adjusted: no
- Suspended solids concentration: 3.6 g/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2 L glass brown-colored flasks
- Number of culture flasks/concentration: 2 bottles
- Method used to create aerobic conditions: The day before incubation the flasks were filled with mineral medium and inoculum and aerated overnight with CO2-free synthetic air.
- Details of trap for CO2 and volatile organics if used: Three CO2 absorbers were connected in series to the exit air line of the bottles (filled with 0.0125 M Ba(OH)2). The CO2 produced in each test bottle reacted with the barium hydroxide in the gas scrubbing bottle and precipitated out as barium carbonate. The amount of CO2 produced was determined by titrating the remaining Ba(OH)2 with 0.05 M standardized HCl (1:20 dilution from 1 M HCl (Titrisol® ampoule), Merck, Darmstadt, Germany).
- Other:
SAMPLING
- Sampling frequency: Titrations were made every second or third day during the first 10 days, and thereafter at least every fifth day until day 28, for the inoculum blank and test suspension. Titrations for the positive and toxicity control were made over a period of at least 14 d.
- Sampling method: Each time the CO2-absorber nearest to the test bottle was removed for titration; each of the remaining two absorbers was moved one position in the direction of the test bottle. A new CO2-absorber was placed at the far end of the series. Phenolphthalein (1% solution in ethanol, Merck) was used as pH-indicator. On day 28, the pH of all test suspensions was measured and 1 mL of concentrated HCl (37%, Merck) was added to the bottles of the inoculum blank and test suspension. The bottles were aerated overnight to drive off CO2 present in the test suspension. The final titration was made on day 29.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 bottles
- Toxicity control: yes, 1 bottle
- Other: reference substance: yes, 1 bottle
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 17
- Sampling time:
- 29 d
- Remarks on result:
- other: Mean of two replicates
BOD5 / COD results
- Results with reference substance:
- The reference substance was degraded to 79% after 14 d confirming the suitability of the inoculum.
Any other information on results incl. tables
The substance is not readily biodegradable (17% degradation after 28 d). However, it has to be considered that substance is inhibitory to the inoculum since the toxicity control showed degradation < 25% after 14 d (12% based on ThCO2). Thus, the degradation result is expected to underestimate the biodegradation of the substance. It is likely that the substance shows a higher degradation if the study is performed with a lower concentration of the test item.
Table 1: Biodegradation of the test item during the study
Day |
Biodegradation [%] |
||
Bottle A |
Bottle B |
Mean |
|
1 |
1 |
0 |
1 |
4 |
3 |
2 |
3 |
6 |
5 |
2 |
4 |
8 |
6 |
2 |
4 |
11 |
8 |
3 |
6 |
14 |
11 |
5 |
8 |
18 |
13 |
5 |
9 |
22 |
13 |
7 |
10 |
27 |
15 |
10 |
13 |
29 |
21 |
13 |
17 |
Table 2: Biodegradation in the toxicity control
Day |
Biodegradation [%] |
1 |
0 |
4 |
4 |
6 |
5 |
8 |
6 |
11 |
10 |
14 |
12 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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