Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 473-780-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 5 March to 9 March 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, GLP compliant; no restrictions, fully adequate for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were taken from the solvent control and each replicate test vessel at 0, 24, 48, 72 and 96 hours for quantitative analysis.
Two samples of the solvent control and each replicate were taken at each occasion. One sample was analysed untreated and one sample after centrifugation (40000 g for approximately 30 minutes).
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The test concentration of 0.0015 mg/l was the highest attainable test concentration due to the limited solubility of the test material in water and auxiliary solvent (dimethylformamide), and having due regard for the amount of auxiliary solvent permitted in the test under the OECD Guidelines.
In a range-finding test fish were exposed to a nominal test concentration of 0.0015 mg/l. A single test concentration was used as results from the range-finding test for the Acute Toxicity to Daphnia magna study (Safepharrn Laboratories Project Number: 0656/0328) indicated that toxicity was not expected at this concentration. The test material was prepared using a preliminary solution in dimethylformamide.
During the definitive test samples of the test preparations were analysed untreated and following centrifugation at 40000 g for 30 minutes. The untreated samples indicated the total amount of test material present in the test preparations whilst the centrifuged samples indicated the amount of soluble and hence bioavailable test material present.
Analysis of the untreated freshly prepared media at 0, 24, 48 and 72 hours showed measured test concentrations of 94% to 136% of the nominal value, indicating that the test system was correctly dosed. Analysis of the freshly prepared centrifuged test preparations at 0, 24, 48 and 72 hours showed measured test concentrations of 31% to 50% of the nominal value.
Analysis of the untreated old test media samples at 24, 48, 72 and 96 hours showed concentrations of 53% to 108% of nominal whilst analysis of the corresponding centrifuged samples showed measured test concentrations of 24% to 52% of the nominal value.
The low measured concentrations in the centrifuged samples for both the freshly prepared and old media indicated that not all the test material present was dissolved. Pre-study work showed that prolonged stirring did not increase the dissolved test material concentration. There was also a general slight decline in the measured test concentrations of the centrifuged test samples over the test period, indicating a decrease in concentration of the soluble test material.
Given that the preliminary stability analyses conducted indicated that the test material was stable in dechlorinated tap water over the media renewal period this decline in measured concentrations was considered to be due to possible adsorption of the test material to glassware as the test material was shown to have a log Koc of 4.57 (Safepharrn Laboratories Project Number 0656/0322).
The inconsistency with the water solubility value of 0.00151 mg/l from the study to determine the water solubility (Safepharm Laboratories Project Number 0656/0322) of the test material was considered to be due to differences in the diluents used, as dechlorinated tap water has a greater dissolved salt/mineral concentration that glass double distilled water used for the water solubility test which may have affected the solubility of the material.
Given this general decline in measured test concentrations it was considered justifiable to base the results on the time weighted mean measured test concentrations of the centrifuged test media to give a "worst case" analysis of the data.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK
- Length at study initiation: 4.5 cm (sd = 0.4)
- Weight at study initiation: 1.32 g (sd = 0.42)
- Feeding during test (discontinued approximately 24 hours prior to the start of the definitive test)
- Food type: Commercial trout pellets
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: Same as test
- Type of food: Commercial trout pelletes
- Health during acclimation (any mortality observed): No mortality in the 7 days prior to the start of the test
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- 140 mg/l as CaCO3
- Test temperature:
- Approximately 14ºC
- pH:
- 7.8 - 8.0
- Dissolved oxygen:
- 9.4 - 10.5 mg O2/l
- Nominal and measured concentrations:
- Nominal: 0.0015 mg/l
Mean measured: 0.00056
See also 'Details on test solutions' - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: Covered
- Material, size, headspace, fill volume: 20 litre, glass
- Aeration: Via narrow bore glass tubes
- Renewal rate of test solution: Test solutiond replaced every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16 hours light, 8 hours dark
EFFECT PARAMETERS MEASURED: Mortality, after 3, 6, 24, 48, 72, 96 hours - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.001 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Absence of any sublethal effects
- Results with reference substance (positive control):
- Not conducted due to ethical reasons
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Although ECHA is providing a lot of online material in your language, part of this page is only in English. More about ECHA’s multilingual practice.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
the-echa-website-uses-cookies
find-out-more-on how-we-use-cookies