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Diss Factsheets
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EC number: 414-310-2 | CAS number: 191358-81-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-12-14 to 2005-01-11
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed under GLP and according to OECD Guideline 305. Even though Tween 80 was used as solubilizer, the test is regarded as valid since the lower test concentration is below the water solubility.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Version / remarks:
- adopted on 14 June, 1996
- Deviations:
- no
- GLP compliance:
- yes
Test material
Constituent 1
- Radiolabelling:
- no
Sampling and analysis
- Details on sampling:
- - Sampling intervals/frequency for test organisms: on day 7, 14, 21, 23, and 28
- Sampling intervals/frequency for test medium samples: on day 0, 7, 14, 21, 23, and 28
- Sample storage conditions before analysis: not applicable
- Details on sampling and analysis of test organisms and test media samples:
Test organisms: sampling on the day of analysis; sample preparation: mincing, homogenization, extraction of test substance to receive a fixed volume solution; HPLC analysis of the generated solution
Test media: preparation of the test water with the aim of generating an organic layer and to receive a fixed volume of test sample; HPLC analysis of the generated solution; pH, DO (adjusted to be approximately 7 mg/L) and water temperature in each aquarium were measured and recorded on the same days when water analyses were performed.
Test solutions
- Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: preparation of a stock solution with dispersant and solvent
- Controls: A 250 mg/L aqueous solution of the dispersant was diluted with the test water supply and then continuously supplied to a test aquarium.
- Chemical name of vehicle:
Dispersant: Polyoxyethylenesorbitan monooleate (Tween 80);
Solvent: Methanol
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)):
Stock solution: the maximum tween 80 amount was 5-times of the test substance;
Final test solution: the high concentration level with 0.5 mg/L test substance had a dispersant concentration of 2.5 mg/L and the low concentration level with 0.05 mg/L test substance a dispersant concentration of 0.25 mg/L
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: common carp
- Source: Kitamura Fish Farm (388, Gunchiku 12-bancho, Yatsushiro-shi, Kumamoto)
- Length at study initiation (lenght definition, mean, range and SD): Mean length: 6.2 ± 0.2 cm
- Weight at study initiation (mean and range, SD): Mean bodyweight: 2.6 ± 0.3 g
- Feeding during test: No feed was given on the day before sampling of the test fish.
- Food type: Mixed carp pellets (Suimmy) with crude protein 37.0% or greater and crude fat 2.5% or greater (manufacturer: Nippon Pet Food Co., Ltd.)
- Amount: The test fish were fed pellets in the amount of ca. 2% per bodyweight/day.
ACCLIMATION
- Acclimation period: 7 days before the study
- Acclimation conditions (same as test or not): yes; temperature: 25 ± 2.0°C
- Type and amount of food: Mixed carp pellets (Suimmy) with crude protein 37.0% or greater and crude fat 2.5% or greater (manufacturer: Nippon Pet Food Co., Ltd.); The test fish were fed pellets in the amount of ca. 2% per bodyweight/day.
- Health during acclimation (any mortality observed): less than 5 %
Study design
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
Test conditions
- Hardness:
- No data.
- Test temperature:
- 24.7 ± 0.7°C
- pH:
- pH 7.1 ± 0.2
- Dissolved oxygen:
- 7.4 ± 0.2 mg/L
- TOC:
- The total organic carbon was confirmed to be 10 mg/L or less:
High exposure level 1.518 mg/ L
Low exposure level 0.449 mg/ L
Control (dispersant) group 1.454 mg/L - Salinity:
- No data.
- Details on test conditions:
- Test aquarium:
"Flow-through system"
Made of glass
Volume 100 L (water volume 50 L)
Flow-through system 300 mL/min (432 L/day)
Aquarium No. 1, No. 2 and No. 3 were used for concentration level 1 at a high exposure level, concentration level 2 at a low exposure level and control group, respectively. - Reference substance (positive control):
- not specified
- Details on estimation of bioconcentration:
- Calculation of the bioconcentration factor (BCF) and the bioconcentration factor at steady state (BCFss):
BCF = Concentration of the test substance in fish (µg/g)/ Concentration of the test subsance in aquarium (mg/L)
BCFss = Mean concentration in fish in the steady-state (µg/g) / Mean concentration in aquarium in the steady-state (mg/L)
Results and discussion
Lipid contentopen allclose all
- Lipid content:
- 4.7 %
- Time point:
- start of exposure
- Lipid content:
- 5 %
- Time point:
- end of exposure
Bioaccumulation factoropen allclose all
- Type:
- BCF
- Value:
- >= 7 - <= 12 L/kg
- Basis:
- total lipid content
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: range of BCFss obtained for the main and minor constituents (total of 13) of the test item
- Remarks:
- Conc.in environment / dose:0.5 mg/L
- Type:
- BCF
- Value:
- >= 10 - <= 18 L/kg
- Basis:
- total lipid content
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: range of BCFss obtained for the main and minor constituents (total of 13) of the test item
- Remarks:
- Conc.in environment / dose:0.05 mg/L
Any other information on results incl. tables
The bioconcentration factors (BCFs) of the present study of all components were less than 50 folds both at the high (0.5 mg/L) and low exposure level (0.05 mg/L).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item does not significantly accumulate in organisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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