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EC number: 216-125-0 | CAS number: 1503-48-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well documented, according to OECD guideline and under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Quino[2,3-b]acridine-6,7,13,14(5H,12H)-tetrone
- EC Number:
- 216-125-0
- EC Name:
- Quino[2,3-b]acridine-6,7,13,14(5H,12H)-tetrone
- Cas Number:
- 1503-48-6
- Molecular formula:
- C20H10N2O4
- IUPAC Name:
- quino[2,3-b]acridine-6,7,13,14(5H,12H)-tetrone
- Details on test material:
- - Analytical purity:97.59%
Constituent 1
Test animals
- Species:
- other: Epi-200
- Strain:
- other: normal, human-derived epidermal keratinocytes , cultured to form a model of the human epidermis, consists of organized basal, spinous and granular layers, and a multi-layered stratum corneum
- Details on test animals or test system and environmental conditions:
- MATERIAL and EQUIPMENT
EpiDerm TM 200 kit: MatTek ln Vitro Life Science Laboratories, Bratislava, Slovakia containing: 24 Epi-200 tissues (reconstructed epidermis): surface 0.6 cm2 cultured in Millicells® 0 1 cm
Tissue for MTTreduction control: Epi-200 tissue that is killed by freezing at -20°C
Assay medium: Dulbecco's modified eagle's medium (DMEM); for the assay and for diluting MTT
Wash buffer: Dulbecco's phosphate buffered saline (P8S), w/o Ca2 + , Mg2 +
Extracting agent: lsopropanol p.a.
Detection agent: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 1.0 mg I ml assay medium
Test system
- Type of coverage:
- other: not applicable
- Preparation of test site:
- other: not applicable
- Vehicle:
- other: not applicable
- Controls:
- other: not applicable
- Amount / concentration applied:
- 25 μL de-ionized water was applied first. Thereafter, a bulk volume of 25 μL of the solid test
material was applied with a sharp spoon and homogeneously distributed with the water - Duration of treatment / exposure:
- 1h
- Observation period:
- not applicable
- Number of animals:
- not applicable
- Details on study design:
- Pretest for direct MTT reduction:
To determine whether the test substance is able to reduce MTT directly, the test substance will be incubated with the substrate. Approximately 50 ul is added to 0.9 ml of the MTT solution. The mixture is incubated in the dark at about 37 oc for 55 to 65 minutes. When the color of the mixture turns blue/purple, it is assumed that the test substance can directly reduce MTT.
Preincubation of the tissues:
Corrosion test: At least 1 hour but not more than 1.5 hours before test-substance application, tissues are transferred to 6-well plates with 0.9 mL assay medium and preconditioned in the incubator at 37C°.
Irritation test: on the day of arrival in the laboratory, the tissues will be transferred to sterile 6-well plates with 0.9 mL assay medium and preconditioned in the incubator at 37°C. After 1 hour the pre-incubation medium is replaced with fresh medium and preconditioning continues for 18 ± 3 hours.
MTT incubation:
After the incubation / postincubation period, the assay medium is replaced by 0.3 ml MTT solution and the tissues are incubated in the incubator for 3 hours. After incubation, the tissues are washed with PBS to stop the MTT-incubation.
Detection of MTT metabolism:
The formazan that is metabolically produced by the tissues will be extracted by incubation of the tissues in 2 ml isopropanol at room temperature overnight (corrosion test) or for at least 2 hours on a plate shaker (ca. 120 rpm) (irritation test). After shaking the isopropanol extract and piercing the tissues, 2 aliquots of each extract per tissue will be transferred to a 96-well microtiter plate. The optical density will be determined
spectrophotometrically using a filter with a wavelength of 570 nm
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: other: Viability (%)
- Value:
- 94
- Remarks on result:
- other:
- Remarks:
- Basis: mean. Time point: 1h. Max. score: 100.0. Reversibility: no data. (migrated information)
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
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