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EC number: 203-956-9 | CAS number: 112-30-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04-Apr-1990 to 27-Apr-1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The restrictions were that the range of strains differs from the current guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- (no TA102 or E. coli WP2 uvrA; 2-AA only positive control with S9)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Hexan-1-ol
- EC Number:
- 203-852-3
- EC Name:
- Hexan-1-ol
- Cas Number:
- 111-27-3
- Molecular formula:
- C6H14O
- IUPAC Name:
- hexan-1-ol
Constituent 1
Method
- Target gene:
- Histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S9 Aroclor 1254 induced
- Test concentrations with justification for top dose:
- 1st test: 8, 40, 200, 1000 and 5000 µg/plate
2nd test: 6.25, 25, 100, 400 and 1600 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: the test substance was suspended in sorbitan derivative (Tween 80)/water
- Justification for choice of solvent/vehicle: none stated
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA100, TA1535 without S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA1537 without S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylendiamine in TA98 and TA1538 without S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene in all strains with S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: none
- Exposure duration: 48 hours
NUMBER OF REPLICATES
- 3 - Evaluation criteria:
- Combination of: a) plate background of non-reverted bacteria not showing growth reduction relative to respective negative controls; b) spontaneous mutation rates within historical limits; c) at one or more doses tested the substance causes a 2-fold (TA 100) or 3-fold (other strains) increase in mutation rate above control levels.
- Statistics:
- None
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
None
COMPARISON WITH HISTORICAL CONTROL DATA:
The spontaneous mutation frequencies for untreated and solvent treated cultures were within the historical range for the laboratory for all strains in the absence and in the presence of S9.
Any other information on results incl. tables
No increase in the number of revertants relative to the solvent and negative controls was observed in any strain at any concentration in first and second experiments. The results of the first test are presented in tables 1 and 2 below. The results of the second test, conducted over a smaller range of concentrations, were very similar to the first. Table 1 Experiment 1 Plate incorporation: Number of revertants per plate (mean of 3 plates)
Strain | TA 98 | TA 100 | TA 1535 | ||||||
Concentration µg/plate | + MA | -MA | Toxicity | + MA | -MA | Toxicity | +MA | -MA | Toxicity |
Negative control | 44.3 | 28.7 | no | 59.3 | 78.7 | no | 5.7 | 5.7 | no |
Solvent control | 38.7 | 35.3 | no | 58.0 | 79.3 | no | 4.0 | 6.7 | no |
5000 | 7.7 | 0 | yes | 9.3 | 20.3 | yes | i pp | i pp | yes |
1000 | 20.3 | 5.0 | yes | 55.0 | 54.7 | no | 4.0 | 4.3 | no |
200 | 35.0 | 38.0 | no | 68.3 | 76.0 | no | 7.3 | 4.3 | no |
40 | 39.3 | 31.7 | no | 64.0 | 77.0 | no | 6.7 | 4.3 | no |
8 | 34.3 | 40.0 | no | 61.7 | 73.3 | no | 6.3 | 4.3 | no |
Positive control | 791.7 | 519.0 | no | 765.0 | 223.3 | no | 74.0 | 213.3 | no |
i pp: inhibition of background lawn and pin-point colonies
Table 2 Experiment 1 Plate incorporation: Number of revertants per plate (mean of 3 plates)
Strain | TA 1537 | TA 1538 | ||||
Concentration µg/plate | + MA | -MA | Toxicity | + MA | -MA | Toxicity |
Negative control | 4.0 | 3.7 | no | 15.0 | 10.0 | no |
Solvent control | 3.7 | 3.7 | no | 12.3 | 8.0 | no |
5000 | i pp | 4.3 | yes | 11.3 | i pp | no |
1000 | 3.3 | 3.3 | no | 10.3 | 5.7 | yes |
200 | 4.7 | 2.3 | no | 13.0 | 7.0 | no |
40 | 5.0 | 5.3 | no | 13.3 | 8.0 | no |
8 | 4.0 | 4.3 | no | 11.3 | 8.7 | no |
Positive control | 269.0 | 354.0 | no | 1134.3 | 884.0 | no |
Applicant's summary and conclusion
- Conclusions:
- In a reliable study, the C6 alcohol Lorol C6 98% did not increase the reverse mutation rate in histidine dependent bacterial strains of Salmonella typhimurium at concentrations of up to 5000 µg/plate in the presence or absence of metabolic activation (cytotoxicity observed at 5000 µg/plate).
The study was performed in compliance with GLP. Expected results were obtained with negative (buffer) solvent and positive controls. The repeat experiment gave the same results as the initial test.
It is concluded that the test substance is negative for mutagenicity in bacteria under the conditions of the study.
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