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EC number: 605-296-0 | CAS number: 162627-17-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- adopted 21 January 2000
- Deviations:
- yes
- Remarks:
- The ratio of quartz sand to dry soil has been increased from 10 g/kg to 67 g/kg in order to ensure homogeneous distribution of the test item-sand mixture in the soil. This deviation had no impact on the outcome of the study.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.21 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- Commission Regulation (EC) No. 440/2008
- Deviations:
- yes
- Remarks:
- The ratio of quartz sand to dry soil has been increased from 10 g/kg to 67 g/kg in order to ensure homogeneous distribution of the test item-sand mixture in the soil. This deviation had no impact on the outcome of the study.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature, protected from light
- Stability under test conditions: not applicable (UVCB substance) - Analytical monitoring:
- not required
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: lucerne meal with a C:N ratio of approx. 15.8:1
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: Application solutions for the different test concentrations were prepared by dispersing the test material in dichloromethane. A volume of 20 mL of the respective application solution was applied dropwise on the surface of 50 g of quartz sand and mixed using a mortar. After evaporating the solvent overnight, the test material-quartz sand mixture was thoroughly mixed with 750 g dry soil. The ratio of quartz sand to dry soil has been increased from 10 g/kg to 67 g/kg in order to ensure homogeneous distribution of the test item-sand mixture in the soil. This deviation had no impact on the outcome of the study.
VEHICLE:
- Chemical name of vehicle: dichloromethane
- Evaporation of vehicle before use: yes - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 19.5 – 19.9 °C (mean 19.6 °C)
- Moisture:
- 42% of the maximum water holding capacity (MWHC)
- Details on test conditions:
- TEST SYSTEM
- Testing facility: climate cabinet
- Test container (type, material, size):1L glass incubation flasks stoppered with cotton wool plugs
- Amount of soil: 160.75 g dry soil per flask (incl. 0.75 g lucerne meal and 10 g quartz sand)
- No. of replicates per concentration: 1
- No. of replicates per control: 1
- No. of replicates per vehicle control: 1
SOIL INCUBATION
- Method: bulk
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site: Landwirtschaftliche Untersuchungs- und Forschungsanstalt (LUFA), Speyer/Germany.
- History of site
- Sampling date: 04 October 2016
- Arrival at testing site: 10 October 2016
- Vegetation cover: no cultivation between 2012 and 2016
- Treatments with pesticides or fertilizers: no pesticides between 2012 and 2016; no fertilizers between 2015 and 2016
- Depth of sampling: approx. 20 cm
- Soil texture
- % sand: 59.6 ± 1.4
- % silt: 33.6 ± 0.5
- % clay: 6.8 ± 1.6
- Soil taxonomic classification: sandy loam
- Soil classification system: USDA
- pH (in water): 6.7
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight):
control: 41.3 mg NO3-/kg dry soil
solvent control: 43.1 mg NO3-/kg dry soil
treated soil: 48.9 – 51.8 mg NO3-/kg dry soil
- Maximum water holding capacity (in % dry weight): 35.6%
- Cation exchange capacity: 7.6 ± 0.8 meq/100 g
- Pretreatment of soil: sieved to 2 mm;
- Storage (condition, duration):
After arrival of the soil at the testing laboratory, the soil was stored in the dark under aerobic conditions at approx. 4°C for three days.
- Initial microbial biomass as % of total organic C: 4.5 %
DETAILS OF PREINCUBATION OF SOIL (if any): the soil was acclimatised to the test conditions (dark, approx. 20°C) for 27 days until test start on (DAT 0)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : nitrate formation after 28 days of exposure
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY: not performed - Nominal and measured concentrations:
- nominal: 62.5, 125, 250, 500, and 1000 mg/kg dry soil
- Reference substance (positive control):
- yes
- Remarks:
- 6.7 mg nitrapyrin/kg dry soil
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: nitrate concentration at Day 28
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: nitrate concentration at Day 28
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: nitrate concentration at Day 28
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: nitrate formation rate inhibited by 166% versus control - Reported statistics and error estimates:
- Differences in nitrate concentrations were tested
- between the control and the solvent control using Student t-tests (two-sided at DAT 0 and DAT 28, α = 0.05);
- between the test item treatments and the solvent control using Dunnett t-tests (two-sided at DAT 0 and DAT 28, α = 0.05);
- between the solvent control and the reference item treatment using Student t-tests (two-sided at DAT 0, one-sided smaller at DAT 28, α = 0.05).
NOEC and LOEC were determined according to the results of the statistics.
Calculations of ECx of nitrate concentration at day 28 were performed using probit analysis and linear maximum likelihood regression). - Validity criteria fulfilled:
- yes
- Remarks:
- The variation between replicate control samples was less than ± 15%.
Reference
No nitrites were detectable in the soil solutions (since nitrites may impact the colorimetric analysis of nitrates, nitrite analysis was performed beforehand).
Description of key information
Toxicity to soil microorganisms: not toxic to soil microorganisms; EC25 for nitrate formation: 1000 mg/kg (OECD 216)
Key value for chemical safety assessment
Additional information
As the test material showed no adverse effects towards soil microorganisms at the highest recommended concentration (EC25 = 1000 mg/kg dry soil weight) in laboratory tests, no NOEC and thus no PNEC could be derived.
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