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EC number: 206-557-8 | CAS number: 354-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Pentafluoroethane
- EC Number:
- 206-557-8
- EC Name:
- Pentafluoroethane
- Cas Number:
- 354-33-6
- Molecular formula:
- C2HF5
- IUPAC Name:
- 1,1,1,2,2-pentafluoroethane
- Details on test material:
- - Name of test material (as cited in study report): HFC-125
- Substance type: organic
- Physical state: Liquefied gas
- Analytical purity: 99.63% w/w
- Impurities (identity and concentrations): Flon 115 (monochloro-pentafluoro-ethane), 0.369% w/w
- Isomers composition: n/a
- Purity test date: not reported
- Lot/batch No.: C-9931
- Expiration date of the lot/batch: not reported
- Storage condition of test material: Cool dark place
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Return sludge from the city sewage plant of Tsubuku (Japan).
- Storage conditions: inoculum kept under aerobic conditions at room temperature until used
- Storage length: less than 1 day
- Preparation of inoculum for exposure: Inoculum sample was filtered, the first 200ml were disregarded and the rest was used as the inoculum
- Pretreatment: no
- Concentration of sludge: not reported
- Initial cell/biomass concentration: 2.8 x 10exp8 bacteria/ml
- Water filtered: purified water was used. No details are available
- Type and size of filter used, if any: not available - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2.99 mg/L
- Based on:
- test mat.
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium with composition as indicated in the OECD GL 301D
- Additional substrate: no
- Solubilising agent (type and concentration if used): not used
- Test temperature: 20 +/-1 °C
- pH: 7.2
- pH adjusted: yes
- CEC (meq/100 g):
- Aeration of dilution water: not reported
- Suspended solids concentration: not calculated
- Continuous darkness: yes
- Other:
TEST SYSTEM
- Culturing apparatus: Incubator Sanyo Electric Co.
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: Not reported
- Method used to create anaerobic conditions: n/a
- Measuring equipment: titration equipment to perform the Winkler method
- Test performed in closed vessels due to significant volatility of test substance: Yes
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: not used
- Other:
SAMPLING
- Sampling frequency: Day 0, 5, 15 and 28 of the study
- Sampling method: for each sample 3 flask/timepoint were prepared
- Sterility check if applicable: n/a
- Sample storage before analysis:not reported
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: No
- Other: positive control, Sodium dodecylsulfate 3 mg/l
Reference substance
- Reference substance:
- laurylsulfonate
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 5
- Sampling time:
- 28 d
- Details on results:
- Degradation of test substance (in %):
= 2 after 5 day(s)
= 5 after 15 day(s)
= 5 after 28 day(s)
Degradation products: no
BOD5 / COD results
- Results with reference substance:
- degradation of reference substance (in %):
= 30 after 5 day(s)
= 92 after 28 day(s)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Not readily biodegradable
- Executive summary:
HFC-125 ready biodegradability was studied by means of a closed bottle test.
The active sludge was sampled from a city sewage plant and filtered. The activated sludge contained 2.8 x 10^8 bacteria/ml. One drop was added to one liter of final test solution. A stock solution of the test substance was prepared by adding gaseous HFC-125 to a vacuum tube containing water until saturation (ca. 0.5 g/l). 600 ul of the stock solution were added to 100 ml of mineral medium containing the inoculum. Sodium laurylsulfate was used as positive control
The test was run at a temperature of 20 ± 1 C.
Biodegradation of tested substance was measured by gas chromatography and biochemical oxygen demand methods.
Degradation of 2, 5 and 5% was calculated from the BOD measurements after 5, 15 and 28 days of incubation of the test substance with the inoculum, respectively. A biodegradation of 30, 57 and 92% was measured for the positive control after 5, 15 and 28 days, respectively.HFC-125 resulted as non readily biodegradable under aerobic conditions.
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