Pentasodium triphosphate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

The results of microbial mutation test for forty-three industrial chemicals

Author:

Shimizu H, Suzuki Y, Takemura N, Goto S and Matsushita H

Year:

1985

Bibliographic source:

Jpn. J. Ind. Health., Vol 27

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Ames test

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Pentasodium triphosphate

EC Number:

231-838-7

EC Name:

Pentasodium triphosphate

Cas Number:

7758-29-4

Molecular formula:

H5O10P3.5Na

IUPAC Name:

pentasodium bis(phosphonatooxy)phosphinate

Details on test material:

- Name of test material (as cited in study report): Pentasodium triphosphate
- Purity: 90%

Method

Species / strain

Species / strain / cell type:

other: S. typhimurium (TA100, TA98, TA1535, TA1537 and TA1538), E.coli (WP2uvrA)

Metabolic activation:

with and without

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

5, 10, 50, 100, 500, 1000 and 5000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water

Controls

Untreated negative controls:

yes

Remarks:

water

Negative solvent / vehicle controls:

yes

Remarks:

DMSO and acetone

Positive controls:

yes

Remarks:

AF-2, ENNG, 9AC, 4NQO B(a)P, 2AA

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation


DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not applicable

Test results 2

Species / strain:

S. typhimurium TA 1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Test results 3

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not applicable

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Table 1: Revertant colonies/plates (without S9 -mix)

Revertant colonies/plates (Mean ± S.D) Without S9-mix
µg/plate	TA100	TA 1535	WP2 uvr A	TA98	TA1537	TA1538
H20 (control)	149 ± 17.1	28 ± 6.9	32 ± 7.3	29 ± 6.2	16 ± 6.4	21 ± 5.5
DMSO (control)	150 ± 16.8	30 ± 5.6	30 ± 9.7	32 ± 7.7	18 ± 8.6	22 ± 7.3
Acetone (control)	179 ± 15.0	23 ± 1.5	30 ± 7.5	24 ± 3.0	10 ± 3.0	21 ± 0.5
AF-2 (0.01)	501 ± 84.7	-	-	-	-	-
AF-2 (0.05)	-	-	1082 ± 293.7	278 ± 64.8	-	-
ENNG	-	1101 ± 683.1	-	-	-	-
9AC	-	-	-	-	889 ± 275.7	-
4NQO	-	-	-	-	-	270 ± 66.2
B(a)P	-	-	-	-	-	-
2AA	-	-	-	-	-	-
5	175	29	22	30	11	21
10	166	26	25	30	20	26
50	167	29	24	37	16	27
100	170	21	30	23	19	25
500	153	26	30	26	21	27
1000	158	24	32	30	13	24
5000	171	21	29	33	12	25

Table 2: Revertant colonies/plate (with S9 mix)

Revertant colonies/plates (Mean ± S.D) With S9-mix
Dose µg/plate	TA100	TA 1535	WP2 uvr A	TA98	TA1537	TA1538
H20 (control)	161 ± 16.2	15 ± 3.6	33 ± 10.3	39 ± 8.6	21 ± 8.1	28 ± 7.0
DMSO (control)	154 ± 17.5	15 ± 7.1	34 ± 10.9	42 ± 10.1	22 ± 6.3	28 ± 5.7
Acetone (control)	136 ± 7.0	8 ± 1.5	26 ± 4.0	32 ± 1.0	18 ± 2.5	26 ± 1.5
AF-2 (0.01)	-	-	-	-	-	-
AF-2 (0.05)	-	-	-	-	-	-
ENNG	-	-	-	-	-	-
9AC	-	-	-	-	-	-
4NQO	-	-	-	-	-	-
B(a)P	1084 ± 236.3	-	-	809 ± 108.4	313 ± 41.6	354 ± 89.4
2AA	-	440 ± 198.6	359 ± 127.0	-	-	-
5	170	16	33	54	23	24
10	172	15	29	63	33	22
50	158	18	27	53	31	19
100	165	19	37	48	25	26
500	182	14	27	49	26	28
1000	175	14	24	53	17	32
5000	166	17	23	59	18	21

- not tested

Positive controls:

2- (2-Furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2), N-ethyl-N'-nitro-N-nitroguanidine (ENNG), 9-aminoacridine (9AC), 4-nitroquinoline-1-oxide (4NQO), benzo(a)pyrene (B(a)P), 2-aminoanthracene (2AA)

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative