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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 203-049-8 | CAS number: 102-71-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Specific investigations: other studies
Administrative data
- Endpoint:
- mechanistic studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of the Potential of Triethanolamine to Alter Hepatic Choline Leveis in Female B6C3F1 Mice
- Author:
- Stott, W.T. et al
- Year:
- 2 004
- Bibliographic source:
- Toxicological sciences 79, 242-247
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The potential of TEA to cause choline deficiency in the liver of mice as a mode of tumorigenesis was investigated. Groups of female B6C3F1 mice were administered 0 (vehicle) or a maximum tolerated dosage (MTD) of 1000 mg/kg bw/day TEA (Trial I) and 0, 10, 100, 300, or 1000 mg/kg bw/day TEA (Trial II) in acetone vehicle via skin painting 5 days/week for 3 weeks. Female CDF® rats were also administered 0 or an MTD dosage of 250 mg/kg bw/day TEA (Trial II) in a similar manner.
- GLP compliance:
- not specified
- Type of method:
- in vivo
- Endpoint addressed:
- carcinogenicity
Test material
- Reference substance name:
- 2,2',2''-nitrilotriethanol
- EC Number:
- 203-049-8
- EC Name:
- 2,2',2''-nitrilotriethanol
- Cas Number:
- 102-71-6
- Molecular formula:
- C6H15NO3
- IUPAC Name:
- 2-[bis(2-hydroxyethyl)amino]ethan-1-ol
Constituent 1
Test animals
- Species:
- other: Mouse and rat
- Strain:
- other: B6C3F1 (mouse) and CDF (rat)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Female B6C3F1/CrIBR® (B6C3F1) mice and CDF® (Fischer 344/CrIBR) (CDF) rats were obtained from Charles River Laboratories Inc. (Portage. MI). Animals were housed one per cage in appropriate stainless steel cages in rooms designed to maintain adequate conditions (temperature, humidity, and photocycle). Animals ware provided LabDiet® Certified Rodent Diet #5002 (PMI Nutrition International, St. Louis, MO) in pelleted form in a hanging feeder und municipal water from a pressure-activated nipple-type watering system ad libitum. This diet contains approximately 1800 ppm choline. Following acclimation, animals (8-12 weeks of age) were stratified using preexposure body weights und ware randomly assigned to treatment groups.
Administration / exposure
- Route of administration:
- dermal
- Vehicle:
- acetone
- Details on exposure:
- An area on the back of each mouse or rat, between the scapulae and stretching posterior approximately halfway to the ileum, was clipped free of hair at least 24 h prior to initiation of dosing. This area was reclipped over the course of the dosing period, as needed, at least 24 hours prior to resumption of dosing. Care was taken to avoid abrasion or nicking of skin. Dosing was as described by NTP (1999a; 2003), by applying (“painting") solutions or vehicle (acetone) directly on the skin using a blunt syringe. Dose solutions in acetone vehicle were applied at a volume of 4 mL/kg (250 mg/mL) in the first mouse trail, 2 ml/kg (5-500 mg/mL) in the second mouse trial, and 0.5 ml/kg (500 mg/mL) in the rat trial. The exposure site was not occluded, nor was any restraining device employed to prevent grooming.
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 3 weeks
- Frequency of treatment:
- 5 days per week
- Post exposure period:
- None
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 mg/kg bw/day (nominal)
- Remarks:
- Trial I (mouse)
- Dose / conc.:
- 1 000 mg/kg bw/day (nominal)
- Remarks:
- Trial I (mouse)
- Dose / conc.:
- 0 mg/kg bw/day (nominal)
- Remarks:
- Trial II (mouse)
- Dose / conc.:
- 10 mg/kg bw/day (nominal)
- Remarks:
- Trial II (mouse)
- Dose / conc.:
- 100 mg/kg bw/day (nominal)
- Remarks:
- Trial II (mouse)
- Dose / conc.:
- 300 mg/kg bw/day (nominal)
- Remarks:
- Trial II (mouse)
- Dose / conc.:
- 1 000 mg/kg bw/day (nominal)
- Remarks:
- Trial II (mouse)
- Dose / conc.:
- 0 mg/kg bw/day (nominal)
- Remarks:
- Trial II (rat)
- Dose / conc.:
- 250 mg/kg bw/day (nominal)
- Remarks:
- Trial II (rat)
- No. of animals per sex per dose:
- Trial I: 10
Trial II: 8 (mouse and rat) - Control animals:
- yes, concurrent vehicle
Results and discussion
- Details on results:
- Clinical Observations and Body Weights
No effects of dosing upon the clinical appearance, body weights, or weight gains of mice or rats were noted. In addition, no evidence of dermal irritation of dosing solutions was noted during the dosing period.
In Vivo Choline and Choline-Related Metabolites
In the initial mouse trial (Trial I), a 1000 mg/kg bw/day TEA dosage caused statistically identified decreases in betaine (26%) and PCho (35%) levels relative to vehicle treated controls. A smaller decrease in hepatic choline concentration (13%) was also observed, which was not statistically identified. In a subsequent dose-response experiment (Trial II mice), all three measured parameters were statistically identified by Trend Test as changing over the dose range, despite a noticeable degree of variability in the data. PCho levels were decreased by 18-20% at 100-300 mg/kg bw/day and by 42% at 1000 mg/kg bw/day compared to controls. Hepatic betaine levels were also decreased across most dosages, with minimal levels observed at the high dosage (29% decrease), and choline levels of high-dose-group mice were depressed by 35% compared to controls. Pairwise statistically significant changes were limited to high-dose groups. Administration of 250 mg/kg bw/day TEA to male CDF rats failed to cause a significant change in any measured parameter.
In Vitro Choline Uptake
TEA caused a statistically identified decrease in the uptake of 3H-choline by growing CHO cells. A dose-related decrease in uptake occurred from 0.67 mM to 1.34 mM concentrations, reaching a maximal inhibition of approximately 60-70% of control at 1.34 to 3.4 mM over the 10-min dosing period. A more pronounced response to DEA was observed, with a dose-related decrease in 3H-choline uptake observed from 0.048 to 0.15 mM, reaching a maximal inhibition of approximately 75% of control at 0.19 to 1.9 mM.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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