Diethyl 4-oxopentane-1,2-dicarboxylate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well conducted GLP compliant study according to international test guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl 4-oxopentane-1,2-dicarboxylate

EC Number:

413-860-0

EC Name:

Diethyl 4-oxopentane-1,2-dicarboxylate

Cas Number:

1187-74-2

Molecular formula:

C11H18O5

IUPAC Name:

1,4-diethyl 2-(2-oxopropyl)butanedioate

Details on test material:

- Name of test material (as cited in study report): acetonyl succinic acid diethyl ester
- Physical state: Liquid
- Analytical purity: 96.1%
- Lot/batch No.: A548702
- Expiration date of the lot/batch: 1992-05-25
- Storage condition of test material: Room temperature, protected from light and humidity

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent from municipal sewage treatment plant: Waterschap de Aa, Schijndel, Netherlands
- Storage conditions: Aeration at 20±1°C
- Preparation of inoculum for exposure: Collected effluent filtered
- Pretreatment: None
- Concentration of sludge: 100µL/L final volume
- Initial cell/biomass concentration:
- Water filtered: yes
- Type and size of filter used, if any: Reverse osmosis followed by activated carbon and ion-exchange cartridges

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

1 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

3 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Solution containing potassium dihydrogen phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, ammonium chloride, magnesium sulphate, calcium chloride and ferric chloride dissolved in water in accordance with test guidelines
- Additional substrate: None
- Solubilising agent (type and concentration if used): None
- Test temperature:20.2 - 20.5 °C
- pH: 7.3 - 7.8
- pH adjusted: no
- Aeration of dilution water: Yes
- Suspended solids concentration: 100µL/L
- Continuous darkness: yes


TEST SYSTEM
- Culturing apparatus: oxygen bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration of medium with oil free air until saturation. Medium left for 67 hours at 20±2°C
- Measuring equipment: Oxygen electrode


SAMPLING
- Sampling frequency: Day 0 (test start), 5, 15 and 28


CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: Yes
- Other: Positive control (sodium acetate)


STATISTICAL METHODS: None

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

-34

Sampling time:

28 d

Remarks on result:

other: 1 mg/L substance

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

-9

Sampling time:

28 d

Remarks on result:

other: 3 mg/L substance

BOD5 / COD results

Results with reference substance:

Biodegradation >60% (72%) within 5 days and 85% within 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test susbtance is not readily biodegradable.
The test substance may be toxic to the bacteria in the inoculum

Executive summary:

The test susbtance is not readily biodegradable.

The test substance may be toxic to the bacteria in the inoculum