2-hydroxy-2-methylpropionitrile

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

chronic toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Principles of method if other than guideline:

Method: Similar to OECD.

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-hydroxy-2-methylpropionitrile

EC Number:

200-909-4

EC Name:

2-hydroxy-2-methylpropionitrile

Cas Number:

75-86-5

Molecular formula:

C4H7NO

IUPAC Name:

2-hydroxy-2-methylpropanenitrile

Details on test material:

- Name of test material (as cited in study report): Acetone Cyanohydrin
- Physical state: liquid
- Analytical purity: 98.5 %
- Purity throughout the test: 98.13 to 97.79 %

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Laboratory, USA, Crl:CD(SD)BR
- Age at study initiation: males: 49 days and females: 54 days of age
- Weight at study initiation: males: 215 - 235 g and females 170 to 189 g
- Fasting period before study: no data
- Housing: individual, all wire, inhalation-type rat cages
- Diet: ad libitum except during the exposure periods
- Water: tap water, ad libitum except during the exposure periods
- Acclimation period: minimum 10 days

ENVIRONMENTAL CONDITIONS
- Temperature (°F): 72 ±2°F
- Humidity (%): 35 - 60 %
- Air changes (per hr): 8 to 15 chamber volumes per hour
- Photoperiod: 12 hrs dark/ 12 hrs light

Administration / exposure

Route of administration:

inhalation

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: 10 m³ Rochester-type stainless steel chambers with pyramidal tops and bottoms

Analytical verification of doses or concentrations:

yes

Details on analytical verification of doses or concentrations:

The infrared method was used to verify the test concentrations containing acetone cyanohydrin in air. Concentrations to be measured are over a range of 5 ppm to 70 ppm in air.

Duration of treatment / exposure:

98 days

Frequency of treatment:

6 hrs/day 5 days/week

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 10.1 (± 0.91 S.D.), 28.6 (± 1.8 S.D.), and 57.7 (± 2.9 S.D.) ppm
Basis:
analytical conc.

No. of animals per sex per dose:

15 male and 15 female rats per dose group

Control animals:

yes, concurrent no treatment

Results and discussion

Results of examinations

Clinical signs:

no effects observed

Mortality:

no mortality observed

Body weight and weight changes:

no effects observed

Description (incidence and severity):

no changes in mean body weights

Effect levels

Dose descriptor:

NOAEL

Effect level:

57.7 ppm

Based on:

test mat.

Sex:

male/female

Target system / organ toxicity

Critical effects observed:

not specified

Applicant's summary and conclusion

Conclusions:

Under the conditions tested in this study a NOEL for toxicity other than irritation and breathing difficulties was established to be 57.7ppm (highest
test concentration) for Acetone cyanohydrin.

Executive summary:

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.