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Diss Factsheets
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EC number: 201-286-1 | CAS number: 80-51-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- Stock solution was prepared by diluting test chemical in dimethylformamide (200,000 mg/L). Test solutions were prepared by adding the corresponding amounts of stock solution into 1,000 mL of OECD medium. The concentration of solvent was 100 µL/L.
OECD M4 medium was applied.
Test concentrations were measured 3 times during 21 days before and after renewal of test solutions - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Age: Juveniles within 24 hrs old
Supplier: Independent Administrative Institution National Institute of Environmental Studies, Japan
Daphnids were fed Chlorella vulgaris for 0.15 mg C /day/animal during the test periods. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- no
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20 ± 1 °C
- pH:
- 7.5 ~ 8.8
- Dissolved oxygen:
- 7.3 ~ 8.6 mg/L (over 60 % of the air saturation value)
- Nominal and measured concentrations:
- Nominal
Con.
(mg/L) Measured Con. (mg/L) TWM** (mg/L) % of Nominal
0 day (new) 1 day (old) 7 day (new) 8 day (old) 14 day (new) 15 day (old)
mg/L (%*) mg/L (%*) mg/L (%*) mg/L (%*) mg/L (%*)
Control <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) – –
Solvent Control <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) – –
0.400 0.370 (93) 0.155 (39) 0.335 (84) 0.143 (36) 0.327 (82) 0.129 (32) 0.229 57
0.850 0.779 (92) 0.332 (39) 0.706 (83) 0.298 (35) 0.760 (89) 0.288 (34) 0.495 58
1.80 1.59 (88) 0.684 (38) 1.68 (93) 0.633 (35) 1.60 (89) 0.674 (37) 1.07 59
3.80 3.32 (87) 1.33 (35) 3.28 (86) 1.32 (35) 3.09 (81) 1.29 (34) 2.13 56
8.00 7.05 (88) 2.74 (34) 6.94 (87) 2.69 (34) 6.53 (82) 2.72 (34) 4.47 56 - Details on test conditions:
- Light: 800 Lux, Light periodicity: 16/8 (light/dark)
- Reference substance (positive control):
- not specified
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.13 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 4.47 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 2.48 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 2.13 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- 21day-EC50 (95 % confidence limits) of OBSH to Daphnia magna was 2.48 mg/L (2.31 ~ 3.63 mg/L). At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.
- Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- The LC50 value and the 95 % confidence limits were calculated by Probit Analysis or Binominal or Moving average method. The EC50 value and the 95 % confidence limits were calculated by Logistic regression analysis method. The NOEC and the LOEC value were analysed by Bartlett test and 1-way ANOVA or Kruskal-Wallis test.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 21 d-NOEC (reproduction) = 2.13 mg/L (TWA)
- Executive summary:
The chronic toxicity of OBSH towards Daphnia magna was assessed applying the OECD Guidline No. 211.
The following concentrations were tested: Control, Solvent control, 0.400, 0.850, 1.80, 3.80 and 8.00 mg/L.
Based on the results, a 21day-NOEC (reproduction) of OBSH to Daphnia magna was determined to be 2.13 mg/L (TWA, semi-static). At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.
Reference
21day-EC50(95 % confidence limits) of OBSH toDaphnia magnawas 2.48 mg/L (2.31~3.63mg/L). At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.
Description of key information
A reliable long-term toxicity study (21 d, semi-static) to Daphnia magna was carried out according to OECD 211, resulting in a 21 d-NOEC (reproduction) of 2.13 mg/L (TWA).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 2.1 mg/L
Additional information
A 21 -d reproduction study on Dapnhia magna showed an NOEC of 2.1 mg/L (Guideline unknown).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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