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EC number: 203-584-7 | CAS number: 108-45-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- Remarks:
- partical size distribution not monitored; temperature, humidity, and pressure in chambers not reported
- GLP compliance:
- not specified
- Test type:
- standard acute method
Test material
- Reference substance name:
- m-phenylenediamine
- EC Number:
- 203-584-7
- EC Name:
- m-phenylenediamine
- Cas Number:
- 108-45-2
- Molecular formula:
- C6H8N2
- IUPAC Name:
- m-phenylenediamine
- Details on test material:
- - Purity: 99.5%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
-Age at study initiation: 7-8 weeks
- Weight at study initiation: 236 - 278 grams
- Fasting period before study: not reported
- Housing: in pairs in 8"x8"x14" stainless steel cages
- Diet (e.g., ad libitum): ad libitum
- Water (e.g., ad libitum): ad libitum
- Acclimation period: 1 week
ENVIRONMENTAL CONDITIONS
-Temperature (ºC): not reported
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): not reported
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- other: Nitrogen
- Details on inhalation exposure:
- Animals were exposed nose-only for single 4-hour periods to atmospheres containing MPD.
Generation: The test material was heated (146-188 ºC) in a round bottom flask. Heated nitrogen (52-75 ºC) was blown through the flask, sweeping MPD vapors into a dilution air stream. Prior to chamber entry, oxygen was added to this mixture to assure a final chamber O2 concentration >20%. - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Test atmospheres were sampled by drawing chamber atmosphere through a fritted glass impinger containing methanol as the trapping solvent. Samples were analyzed on a Hewlett-Packard 5700A Gas Chromotograph equipped with a flame ionization detector.
- Duration of exposure:
- 4 h
- Concentrations:
- Mean: 0.72, 2.0, 3.2, 3.9 mg/L
- No. of animals per sex per dose:
- 10
- Control animals:
- not specified
- Details on study design:
- During exposure all rats were observed, clinical signs noted, and chamber oxygen content and temperature monitored. Following exposure, all surviving rats were weighed and observed for 14 days.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 3.2 mg/L air (analytical)
- 95% CL:
- > 2.6 - < 4.1
- Exp. duration:
- 4 h
- Sex:
- male
- Dose descriptor:
- other: LOAEL
- Effect level:
- 0.72 mg/L air
- Exp. duration:
- 4 h
- Remarks on result:
- other: At all concentrations, rats exhibited red ocular and nasal discharge.
- Mortality:
- Deaths occurred 1-7 days post exposure.
- Clinical signs:
- other: At all concentrations, rats exhibited red ocular and nasal discharge. Rats at all concentrations showed hair loss and wet perineum. Lung noise, brown stained fur, pallor, and tremors were observed at concentrations ≥ 2.0 mg/L. Labored breathing, dry re
- Body weight:
- At all concentrations, rats exhibited moderate to severe weight loss for 1-3 days followed by weight gain.
- Other findings:
- Upon contact with the cool dilution air, the test substance's vapors condensed into an aerosol. The resulting atmosphere was visible to the unaided eye. Chamber temperature was ≤ 31 ºC and oxygen content was ≥ 20% for all exposures.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- 4-hour LC50 = 3.2 mg/L
- Executive summary:
An inhalation Median Lethal Concentration for the test substance was determined in male Crl:CD®rats given single 4 -hour exposures. The calculated LC50 is 3.2 mg/L with 95% confidence limits of 2.6 and 4.1 mg/L.
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