[2-(methacryloyloxy)ethyl]trimethylammonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[2-(methacryloyloxy)ethyl]trimethylammonium chloride

EC Number:

225-733-5

EC Name:

[2-(methacryloyloxy)ethyl]trimethylammonium chloride

Cas Number:

5039-78-1

Molecular formula:

C9H18NO2.Cl

IUPAC Name:

Trimethyl((2-[(2-methylprop-2-enoyl)oxy]ethyl))azanium chloride

Details on test material:

- Name of test material (as cited in study report): MADQUAT MC 75
- Substance type: organic
- Physical state: liquid
- Analytical purity: 75%
- Impurities (identity and concentrations): Water (25%)
- Composition of test material, percentage of components: 100%
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: stable
- Storage condition of test material: at room temperature and away from the light

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: at 0 and 48 hours
- Sample storage conditions before analysis: no data

Test solutions

Vehicle:

yes

Details on test solutions:

Ultrapure water (ultrafiltration, activated carbon, ion exchange, 0.22 micron filter

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Strain: Clone 5 (Clone A)
- Source: In-house
- Age at study initiation: less than 24 hours
- Feeding during test: no
ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): no data
- Type and amount of food: microscopic algae
- Feeding frequency: no data
- Health during acclimation (any mortality observed): no data

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Not specified

Test temperature:

19.5-20.5°C

pH:

7.60 to 7.82

Dissolved oxygen:

8.1 to 8.5 mg O2/l

Nominal and measured concentrations:

Nominal (mg/l): 0, 100, 135, 182.8, 246, 332.1, 448.4
Measured (initial) (mg/l): 0, 104.3, 145.1, 197.6, 267.4, 362.3, 488.2
Measured (final) (mg/l): 0, 91.8, 127.3, 174.0, 237.9, 322.5, 435.6

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 ml beakers
- Aeration: non
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ultrapure
- Total organic carbon: not specified
- Particulate matter: not specified
- Metals: Ca and Mg = 2,5 mmol/l
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: not specified
- Ca/mg ratio: 4
- Conductivity: not specified
- Culture medium different from test medium: no
- Intervals of water quality measurement: not specified
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 24 hours darkness
- Light intensity: not applicable
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilization
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not specified
- Range finding study: yes
- Test concentrations: 0.1 to 100 mg/l
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

104.3 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 488.2 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

104.3 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 488.2 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50/24 h: 0.83 mg/l

Any other information on results incl. tables

Concentration				Immobilisation
Nominal (mg/l)	Measured
	Initial (mg/l)	Final (mg/l)	Final/initial	24 hours (%)	48 hours (%)
448.4	488.2	435.6	89.2	20	25
332.1	362.3	322.5	89.0	15	20
246.0	267.4	237.9	88.9	10	10
182.8	197.6	174.0	88.0	5	10
135.0	145.1	127.3	87.7	5	5
100.0	104.3	91.8	88.0	0	0
0	-	-	-	0	5

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

MADQUAT MC 48 has a low toxicity to Daphnia magna under the test conditions. After 48 hours, the NOEC was 104.3 mg/l and the EC50 was over 488.2 mg/l.